Prostaglandin H synthase
Hypercalcemia stimulates expression of intrarenal phospholipase A2 and prostaglandin H synthase 2 in rats Role of angiotensin II AT1 receptors
... (cPLA2), prostaglandin H synthase-1 (PGHS-1), and prostaglandin H synthase-2 (PGHS-2), enzymes important in prostaglandin ...
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Secretory leukocyte protease inhibitor suppresses the production of monocyte prostaglandin H synthase 2, prostaglandin E2, and matrix metalloproteinases
... of prostaglandin H synthase/cyclooxy- genase (EC ...Two prostaglandin synthase isoforms have been identified, referred to as PGHS-1 and PGHS-2, and are found in many cell types (see ...
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Enhanced prostacyclin synthesis in endothelial cells by retrovirus mediated transfer of prostaglandin H synthase cDNA
... A retroviral vector (BAG) was used to transfer human prostaglandin H synthase (PGHS-1) gene into a human endothelial cell line for enhancement of PGI2 synthesis. Cells infected with BAG containing ...
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Lipopolysaccharide induces prostaglandin H synthase 2 protein and mRNA in human alveolar macrophages and blood monocytes
... We and others have previously demonstrated that human alveolar macrophages produce more PGE2 in response to lipopolysaccharide (LPS) than do blood monocytes. We hypothesized that this observation was due to a greater ...
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Differential impact of prostaglandin H synthase 1 knockdown on platelets and parturition
... PGF2α at 8% WT levels was sufficient to induce coordinated temporal oxytocin receptor OTR expression in uterus and normal ovarian luteolysis in PGHS1Neo/Neo mice at late gestation, while[r] ...
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Allergic lung responses are increased in prostaglandin H synthase–deficient mice
... volume were calculated for each breath cycle. Compli- ance and resistance were calculated using the constant- flow inflation method single-compartment model (24). All values were averaged over 6-second intervals and ...
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Human immunodeficiency virus type 1 envelope protein does not stimulate either prostaglandin formation or the expression of prostaglandin H synthase in THP-1 human monocytes/macrophages.
... ity to form prostaglandins. As seen in Fig. 3, THP-1 monocytes did not significantly metabolize exogenous arachidonic acid. Preincubation of the THP-1 cells with LPS, a known stimulator of PGHS-2 expression in ...
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Influence of natural and recombinant interleukin 2 on endothelial cell arachidonate metabolism Induction of de novo synthesis of prostaglandin H synthase
... HUVECS without affecting thymidine incorporation in lymphocytes. HPLC analysis of arachidonate metabolites detected predominantly 6 keto-PGF1 alpha (6KPGF1 alpha) peak. Treatment of cultured endothelial cells with ...
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Role of intestinal epithelial cells in the host secretory response to infection by invasive bacteria Bacterial entry induces epithelial prostaglandin h synthase 2 expression and prostaglandin E2 and F2alpha production
... and prostaglandin production could pro- tect against mucosal injury is suggested by the mitogenic and antiapoptotic activity of PGHS-2 expressed in epithelial cells (30, ...
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Corticotropin releasing hormone interacts with interleukin 1 to regulate prostaglandin H synthase 2 expression in human myometrium during pregnancy and labor
... Total RNA was extracted from myometrial cell cultures by an RNeasy total RNA kit and reverse transcribed to synthesize cDNA by using RNase H Reverse Transcriptase (Life Technol- ogies). Quantitative PCR was used ...
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sn 1,2 Diacylglycerols and phorbol diesters stimulate thromboxane synthesis by de novo synthesis of prostaglandin H synthase in human promyelocytic leukemia cells
... of prostaglandin H (PGH) synthase because: microsomal PGH synthase activity showed large increases in Vmax values, and mass measurements of PGH synthase revealed the presence of PGH ...
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A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity
... to prostaglandin H 2 (PGH 2 ), the pre- cursor of all ...endoperoxide, prostaglandin G 2 (PGG 2 ), and a haem with peroxidase activity that reduces PGG 2 to PGH 2 (For review see ...
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Protection of rabbit lungs from endotoxin injury by in vivo hyperexpression of the prostaglandin G/H synthase gene
... eicosanoids. In an in situ, perfused preparation of PGH synthase transfected rabbit lungs, the pressor response to endotoxin was markedly attenuated. In addition, pulmonary edema and release of thromboxane B2 into ...
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A Computational Approach to Investigate the Biochemical Properties of Paracetamol and Its Metabolites
... All the metabolites of PCT are thermally and configurationally more stable than PCT (paracetamol). Most of them have higher softness values, which means higher chemical reactivity with lower HOMO-LUMO gap. From the ...
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Concentrations of prostaglandin endoperoxide synthase and prostaglandin I2 synthase in the endothelium and smooth muscle of bovine aorta
... PGI2 synthase and prostaglandin endoperoxide (PGH) synthase in bovine aorta and obtained results quite different from those described in this earlier ...PGI2 synthase and PGH synthase ...
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Transcriptional induction of prostaglandin G/H synthase 2 by basic fibroblast growth factor
... elevations at 48 and 96 h that were inhibited by indomethacin. In both MC3T3-E1 cells and neonatal calvariae, bFGF produced smaller and slower increases in PGHS-1 mRNA levels than for PGHS-2. bFGF stimulated bone ...
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Cyclooxygenases, microsomal prostaglandin E synthase 1, and cardiovascular function
... (PGHS-2; known colloquially as COX-2) increase the incidence of myocardial infarction and stroke. These inhibitors are believed to exert both their beneficial and their adverse effects by suppression of PGHS-2–derived ...
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THE RECENT DEVELOPMENT ON PROSTAGLANDIS AND PROSTANOIDS
... from prostaglandin A2, has been accomplished, as well as an efficient regioselective hydroxylation of the upper chain of the prostanoid ...the prostaglandin was modified in the upper chain, the antimitotic ...
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Exploration of candidate serum biomarkers potentially related to the chronic pain condition in Medication–overuse headache
... lipocalin-type prostaglandin D2 synthase ...of prostaglandin D2 (PGD2), the most abundant prostanoid produced in the central nervous system (CNS) ...entire prostaglandin system has a central ...
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Experimental and bioinformatic analysis of cultured Bovine Endometrial Cells (BEND) responding to interferon tau (IFNT)
... for prostaglandin-endoperoxide synthase 2 (PTGS2), phospholipase A2, group IVA (PLA2G4A), estrogen receptor 1 (ESR1) and progesterone receptor (PR) in response to IFNT in BEND cells and searched for ...
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