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Protein and Immunological Analysis

Molecular and immunological analysis of a polymorphic periplasmic protein of Borrelia burgdorferi

Molecular and immunological analysis of a polymorphic periplasmic protein of Borrelia burgdorferi

... A higher concentration of anti- P22-A antibodies, however, was detected in sera collected at 21 days postinoculation from six mice inoculated with a similar number of P6 cells that had b[r] ...

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BALB/c myeloma retroviruses: peptide mapping and immunological analysis of the pp12 structural protein.

BALB/c myeloma retroviruses: peptide mapping and immunological analysis of the pp12 structural protein.

... the ppi2 proteins of the two myeloma viruses only shared partial antigenic identity with the ppl2 of this amphotropic virus. Thus, the im-[r] ...

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Cloning, Expression, and Immunological Characterization of the P30 Protein of Mycoplasma pneumoniae

Cloning, Expression, and Immunological Characterization of the P30 Protein of Mycoplasma pneumoniae

... P30 protein have mainly been fo- cused on understanding its role in cytadherence and gliding motility (9, 11, 16, ...detailed immunological analysis of the rMPN30–pMAL-p2x fusion ...fusion ...

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Immunological analysis of aerobic bioreactor bovine theileriosis vaccine.

Immunological analysis of aerobic bioreactor bovine theileriosis vaccine.

... DTH is a cell-mediated response that a small amount of protein extracted from the micro- organism, is injected into the skin. The test is positive when the case is infected now, or has been infected previously. ...

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Immunological analysis of drugs of abuse with reference to anhydroecgonine methyl ester

Immunological analysis of drugs of abuse with reference to anhydroecgonine methyl ester

... synthesis of the immunogen it was expected that the antibodies produced would show equal specificity to both AEME and AE. This was not the case, AE only showed 0.6% cross reactivity at lOOOng/mL. The dose response curve ...

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Mosquito salivary gland protein preservation in the field for immunological and biochemical analysis

Mosquito salivary gland protein preservation in the field for immunological and biochemical analysis

... Thus, to avoid the risk of mosquito settlement by bring- ing larvae outside their natural habitat in an area exempt of this species, collection of SG in the field appears more reasonable. However, SG dissection cur- ...

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Protein Structure Facilitates High-Resolution Immunological Mapping

Protein Structure Facilitates High-Resolution Immunological Mapping

... Chemically inactivated formalin toxoid vaccines continue to be used to neutralize diphtheria toxin and tetanus toxin. This strategy was used for botulinum toxoids, but long-term storage resulted in decreased potency and ...

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Functional and Immunological Relevance of Anaplasma marginale Major Surface Protein 1a Sequence and Structural Analysis

Functional and Immunological Relevance of Anaplasma marginale Major Surface Protein 1a Sequence and Structural Analysis

... Abstract Bovine anaplasmosis is caused by cattle infection with the tick-borne bacterium, Anaplasma marginale. The major surface protein 1a (MSP1a) has been used as a genetic marker for identifying A. marginale ...

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The effect of microfluidization on liposomal surface protein: characterization and immunological studies

The effect of microfluidization on liposomal surface protein: characterization and immunological studies

... PREPARATION OF LIPOSOMES Most methods of preparing liposomes basically involve three or four stages: drying down of lipids from organic solvents, dispersion of the lipids in aqueous media, isolation of the resultant ...

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Late nonstructural 100,000- and 33,000-dalton proteins of adenovirus type 2. II. Immunological and protein chemical analysis.

Late nonstructural 100,000- and 33,000-dalton proteins of adenovirus type 2. II. Immunological and protein chemical analysis.

... seems to be within the region encoding the 100K protein (17); (iii) Axelrod (2) analyzed the tryptic peptides of Ad2 lOOK and 33K proteins labeled.. with a '4C-amino acid mixture and fou[r] ...

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Expression and Immunological Characterization of the Carboxy Terminal Region of the P1 Adhesin Protein of Mycoplasma pneumoniae

Expression and Immunological Characterization of the Carboxy Terminal Region of the P1 Adhesin Protein of Mycoplasma pneumoniae

... Toward this end, we expressed two domains of P1 gene, one in the N-terminal region and another in the C-terminal region. Both the fragments were expressed in E. coli by using the pQE-30 expression system. In our study, ...

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Construction, Expression and Preliminary Immunological Evaluation of a DNA Plasmid Encoding the GRA2 Protein of Toxoplasma gondii

Construction, Expression and Preliminary Immunological Evaluation of a DNA Plasmid Encoding the GRA2 Protein of Toxoplasma gondii

... GRA2 protein by mammalian cells in ...immunoblot analysis, a pool of sera from 5 mice immunized with pcGRA2 reacted strongly with a protein band at the expected molecular mass of GRA2 (28 kDa) ...

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Immunological characterization of Plasmodium vivax Pv32, a novel predicted GPI anchored merozoite surface protein

Immunological characterization of Plasmodium vivax Pv32, a novel predicted GPI anchored merozoite surface protein

... surface protein that was frequently exposed to the host immune ...functional analysis of Pv32 remains to be investigated regarding whether the anti- Pv32 antibody could inhibit parasite invasion or even be ...

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Immunological characterization of recombinant Salmonella enterica serovar Typhi FliC protein expressed in Escherichia coli

Immunological characterization of recombinant Salmonella enterica serovar Typhi FliC protein expressed in Escherichia coli

... Though fliC gene was cloned and expressed in E. coli successfully, but further analysis revealed that most of the rFliC was present in inclusion bodies or insoluble fraction. Changing poly-histidine tag from ...

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Immunological markers of Plasmodium vivaxexposure and immunity: a systematic review and meta-analysis

Immunological markers of Plasmodium vivaxexposure and immunity: a systematic review and meta-analysis

... The included studies reported data from Brazil (n = 10), Papua New Guinea (n = 4), Indonesia (n = 2), Thailand (n = 2), and Turkey (n = 1) (Table 1). Sample sizes of the included studies ranged from 61 to 432 study par- ...

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Vaccination and immunological memory

Vaccination and immunological memory

... control protein mouse ...a protein (in this case an autologous protein) could itself induce activation of the OT-II ...Cytometry Analysis) and to quantify endogenous T cell responses against ...

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Immunological Markers of the R4 Protein of Streptococcus agalactiae

Immunological Markers of the R4 Protein of Streptococcus agalactiae

... on immunological markers of R4, an important Streptococcus group B (GBS) ...alpha-like protein 3 (Alp3) of GBS, was named R4/Alp3 common, and was expressed by GBS, which possessed the Alp3-encoding gene ...

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Immunological investigations into the properties and function of the phosphatidylinositol transfer protein

Immunological investigations into the properties and function of the phosphatidylinositol transfer protein

... length protein is accompanied by an increase in the am ount o f im m unoreactive material at approximately 100 kD a which is believed to represent a proteolytic fragment o f ...

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Immunological Studies of Y Protein  A MAJOR CYTOPLASMIC ORGANIC ANION BINDING PROTEIN IN RAT LIVER

Immunological Studies of Y Protein A MAJOR CYTOPLASMIC ORGANIC ANION BINDING PROTEIN IN RAT LIVER

... Y protein, the major cytoplasmic organic anion-binding protein in liver, was ...Y protein from liver supernatant fractions and specifically removed the organic anion-binding capacity from this ...

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Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus

Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus

... Further characterization was done with various sera against other animal CoVs, and no cross-reaction was detected be- tween the C fragment of S protein and other related CoV- infected animal serum samples. Thus, ...

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