Protein Translocation
Hsp70 at the membrane: driving protein translocation
... As described above, considerable progress has been made towards understanding the action of Hsp70s in protein translocation on both sides of membranes. Many ques- tions remain, however. On the cytosolic ...
11
Sec-facilitated Protein Translocation and Membrane Integration
... membrane protein topogenesis and TM stop-transfer ef- ...for protein integration, including a mechanism in which the nascent protein undergoes slow-timescale reorientation, or flip- ping, in the ...
219
Application of phiLOV2.1 as a fluorescent marker for visualization of Agrobacterium effector protein translocation
... nuclear marker, but only few phiLOV2.1-VirF translocation events could be found. After co-cultivation with LBA1010 lacking any phiLOV2.1 protein, as expected, no phiLOV2.1 fluorescence could be detected ...
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Dynamic action of the Sec machinery during initiation, protein translocation and termination
... the protein translocation rate has been undermined by problems inherent to ensemble analysis of unsynchronised reactions; wherein multiple steps are con- voluted into one overall measurable value; that is ...
26
ATP induced asymmetric pre protein folding as a driver of protein translocation through the Sec machinery
... The results described here allow us to extend our previously proposed ‘Brownian ratchet’ model for protein translocation (Allen et al., 2016), outlined in Figure 8. The initiation process involves ATP ...
25
Duplication and Suppression of Chloroplast Protein Translocation Genes in Maize
... membrane protein required for translocation of a subset of proteins across the thylakoid ...Sec-dependent protein translocation pathways provide evidence that they act ...
12
Characterization of Two Putative Protein Translocation Components in the Apicoplast of Plasmodium falciparum
... PfTic22 protein, which is con- sistent with retention of the native promoter during integration of the tag ...with protein amounts peaking at approximately 24 to 28 h, the early- to mid-tropho- zoite stage ...
9
Analysis of Polypeptide movement in the SecY channel during SecA mediated protein translocation
... SecA is a multidomain protein that contains two nucleotide binding folds (NBF1 and 2) with the nucleotide binding site in between them (10). ATP binding and hydrolysis are linked to conformational changes of the ...
8
EspZ of Enteropathogenic and Enterohemorrhagic Escherichia coli Regulates Type III Secretion System Protein Translocation
... EPEC and enterohemorrhagic E. coli (EHEC) are important human pathogens. While EPEC strains cause bacterial pediatric diarrhea and mortality in developing countries (8), EHEC strains, particularly those belonging to ...
12
The Relationships between Energy Balance Deviations and Adiposity in Children and Adolescents
... Sec-dependent translocation depicts the bacterial SecYEG complex as providing the essential, protein-conducting channel, with SecA acting as a peri- pheral component of the translocase hydrolyzing ATP (Chen ...
172
Membrane Heredity Composed by Symbiogenesis
... eukaryotic protein translocation machineries reveals the real ingenuity of symbiogenetic ...eukaryotic protein translocation molecular assemblies in eukaryotes: (i) the ER-associated ...
7
MBAR enhanced lattice Monte Carlo simulation of the effect of helices on membrane protein aggregation
... in the sense that the process is irreversible, there are cases where proteins are recruited only to form a functional aggregate, and then dissociated back into a monomeric state once that functional role is fulfilled. ...
34
Electrically facilitated translocation of protein through solid nanopore
... the protein is likely to enter the pore and pass through ...for protein translocation through ...Thus, protein translocation through nanopores crosses over from almost purely diffusive ...
10
Coarse-Grained Simulation Approaches for Protein Integration and Translocation via the Sec Translocon
... dual-topology protein EmrE [129], and the effect of sequence modifications on the integration efficiency of the multispanning protein TatC ...co-translational protein translocation and ...
135
How translocation affects the activity, home range and pair-bonds of great spotted kiwi (Apteryx haastii)
... post translocation, as is the case for Bella, Feisty and Lichen, or returned to their previously held territory by the end of the study, as in the case of ...pre translocation territory, where he remained ...
113
Direct interaction between p53 and Tid1 proteins affects p53 mitochondrial localization and apoptosis
... This translocation was a general phenomenon to wildtype p53 harboring malignant and non-malignant cells and occurred prior to mitochondrial membrane potential changes, the apoptotic release of cytochrome c, and ...
9
Methylation of RCAN1.4 mediated by DNMT1 and DNMT3b enhances hepatic stellate cell activation and liver fibrogenesis through Calcineurin/NFAT3 signaling
... a week. To investigate the functional role of RCAN1.4 in the progression of liver fibrosis, we specifically over-expressed RCAN1.4 in mice liver using rAAV8-packaged RCAN1.4 over-expression plasmid. Following the ...
16
Blocking of Interferon-Induced Jak-Stat Signaling by Japanese Encephalitis Virus NS5 through a Protein Tyrosine Phosphatase-Mediated Mechanism
... Stat2 protein expression (19, ...flavivirus protein (905 aa long in the case of JEV) and contains the enzymatic activ- ities of MTase (aa 54 to 228) and RdRP (aa 253 to 901) at its N-terminal and C-terminal ...
11
Second-Generation HSP90 Inhibitor Onalespib Blocks mRNA Splicing of Androgen Receptor Variant 7 in Prostate Cancer Cells.
... AR-V7 protein in onalespib- treated cells was the result of altered protein stability, we treated VCaP with the protein synthesis inhibitor cycloheximide (CHD) in the presence or absence of onalespib ...
23
The 2B subdomain of Rep helicase links translocation along DNA with protein displacement
... Rep2B is defective in accessory replicative helicase activity We tested the ability of wild type Rep and Rep2B to pro- mote replication of protein-bound DNA. EcoRI E111G binds to its cognate DNA sequence but has ...
10