Proteinase 3
Proteinase 3 antineutrophil cytoplasmic antibody positive necrotizing crescentic glomerulonephritis complicated by infectious endocarditis: a case report
... microscopy; ESR: Erythrocyte sedimentation rate; F: Female; FSGS: Focal segmental glomerulonephritis; GBM: Antiglomerular basement membrane antibody; GN: Glomerular nephritis; HbA1c: Hemoglobin A1c; HPF: High- power ...
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What you should know about PR3 ANCA: Structural aspects of antibodies to proteinase 3 (PR3)
... anti-proteinase 3 (PR3) antibody contained many negatively charged aspartic acid residues, perhaps contributing to its reactivity with positively charged PR3 ...
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Proteinase 3 and neutrophil elastase enhance inflammation in mice by inactivating antiinflammatory progranulin
... inflammation. Proteinase 3 (PR3) and neutrophil elastase (NE) are 2 abundant neutrophil serine proteases implicated in antimicrobial defense with overlapping and potentially redundant substrate ...
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Identification and Validation of Proteinase 3 and Latent Matrix-Metalloproteinase 9 as Potential Biomarkers for Chronic Lung Transplant Rejection
... The ELISA assay of proteinase 3 was constructed in- house following the supplier’s protocol for assay set-up. Highly purified human PR-3 antibody from the supplier was reconstituted in 2 ml distilled ...
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EXPRESSION OF HUMAN PROTEINASE 3 IN CHINESE HAMSTER OVARY CELLS (CHO-CELLS)
... Proteinase 3(PR3) is a human polymorphonuclear leukocyte serine pro teinase and is the main target antigen for antineutrophil cytoplasmic antibod ies (ANCA) found in Wegener's granulomatosis ...
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Anti proteinase 3 anti neutrophil cytoplasm autoantibodies recapitulate systemic vasculitis in mice with a humanized immune system
... Evidence is lacking for direct pathogenicity of human anti-proteinase-3 (PR3) antibodies in development of systemic vasculitis and granulomatosis with polyangiitis (GPA, Wegener’s granulomatosis). Progress ...
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Extraction and Purification of Anti-Proteinase 3 (PR3) Antibodies from Egg Yolk
... tests. Proteinase 3 (PR3), a constituent of azurophil granules of neutrophils, is the target antigen for most anti-neutrophil cytoplasmic antibodies (c-ANCA) in Wegener granulomatosis ...
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Activation of Proteinase 3 Contributes to Nonalcoholic Fatty Liver Disease and Insulin Resistance
... of proteinase 3 in diet-induced NAFLD and insulin resis- tance, we first examined proteinase 3 messenger RNA (mRNA) expression in liver tissues obtained from independent studies investigating ...
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Monitoring Proteinase 3 Antineutrophil Cytoplasmic Antibodies for Detection of Relapses in Small Vessel Vasculitis
... anti-proteinase 3 (anti-PR3) monoclonal antibody 4A3 has previously been proven to be superior to indirect immunofluorescence (IIF) and standard ELISA for the diagnosis of ...
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Proteinase 3 A distinct human polymorphonuclear leukocyte proteinase that produces emphysema in hamsters
... designated proteinase 3 (PR-3) was purified, characterized in vitro, and tested for its ability to cause ...serine proteinase with isoenzyme ...
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Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis
... inflammation and the presence of anti-neutrophil cytoplasmic antibodies (ANCAs) directed against proteinase 3 (PR3). We previously determined that PR3 on the surface of apoptotic neutrophils interferes with ...
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Proteinase 3–dependent caspase 3 cleavage modulates neutrophil death and inflammation
... physiopathological inflammatory responses; however, the underlying regulatory pathways remain ill defined. Here we determined that in aging neutrophils, the cleavage and activation of caspase-3 is independent of ...
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Proteinase 3 as the major autoantigen of c ANCA is strongly expressed in lung tissue of patients with Wegener's granulomatosis
... serine proteinase present in azurophil granules of human polymorphonuclear leukocytes and serves as the major target antigen of antineutrophil cytoplasmic antibodies with a cytoplasmic staining pattern (c-ANCA) in ...
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Proteinase 3; a potential target in chronic obstructive pulmonary disease and other chronic inflammatory diseases
... In addition, PR3 can adhere to neutrophil extracellular traps (NETs) contributing towards the destruction of bacterial virulence factors [80–82]. However, many respiratory-relevant bacteria, such as Streptococcus ...
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Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis
... For quantitative RT-PCR, leukocyte RNA was analyzed from 40 PR3-ANCA patients and 40 MPO-ANCA pa- tients (each group of 40: remission (BVAS = 0), n = 20; active (BVAS ≥ 3), n = 20), and 20 healthy controls. ...
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Hallmark trials in ANCA-associated vasculitis (AAV) for the pediatric rheumatologist
... Cyclophosphamide (CYC) is one of the most com- monly used and well-studied induction therapies. All CYC trials were conducted with one shared aim: to achieve disease remission and minimize medication tox- icity. CYCLOPS ...
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Anti-neutrophil cytoplasmic antibodies in rheumatoid arthritis: two case reports and review of literature
... Rheumatoid arthritis (RA) is a systemic inflammatory autoimmune disease characterized by chronic polyarthri- tis, eventually leading to joint erosion, and by the pre- sence of various autoantibodies. The autoantigens ...
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Index Catalog // Carolina Digital Repository
... ANCA glomerulonephritis is the most common form of aggressive glomerular disease and is associated with a systemic necrotizing vasculitis including microscopic polyangiitis (MPA) and granulomatosis with polyangiitis ...
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ANCA Associated Vasculitides—An Update
... tigen Proteinase-3 and neutrophilic respiratory burst can be stimulated by binding of ANCA, it is generally ac- cepted, that an initial priming step is required for their pathophysiological function in AAV ...
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Use of a Granulocyte Immunofluorescence Assay Designed for Humans for Detection of Antineutrophil Cytoplasmic Antibodies in Dogs with Chronic Enteropathies
... An assay b not previously reported in the veterinary literature for dogs was utilized for analysis of antibodies in the serum sam- ples. Granulocyte mosaic slides containing biochips with human granulocytes fixed in ...
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