Proteinase K
Comparative Studies on the Interaction of Proteinase-K with Fe2O3, Fe3O4 and SiO2 Nanoparticles
... using UV–vis spectroscopy. Proteinase K EC (3.4.21.14) is a member of serine protease family, which is produced from fungus Tritirachum album Limber. The effects of nanoparticles on proteinase ...
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Comparative Studies on the Interaction of Proteinase-K with Nano-CuO and Copper Ions
... and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics ...on proteinase K stability and ...
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Distinct Proteinase K Resistant Prion Protein Fragment in Goats with No Signs of Disease in a Classical Scrapie Outbreak
... First, the degrees of tissue autolysis were similar for samples of classical-scrapie-affected goats and those with the ⬃12-kDa PrPres; second, the truncated fragment was also identified [r] ...
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Serological cross-reactions between Coxiella burnetii and Legionella micdadei.
... with proteinase K indicated that cross-reactions were probably due to both protein and lipopolysaccharide antigens, because removal of protein antigens prior to immunoblotting removed all bands but ...
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Identification of Acinetobacter Isolates from Species Belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex with Monoclonal Antibodies Specific for O Antigens of Their Lipopolysaccharides
... with proteinase K-treated lysates from a large collection of Acinetobacter isolates (n ⴝ 631) to determine whether the antibodies could be used for the reliable identification of strains from this ...
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Receptor-Induced Conformational Changes of Murine Coronavirus Spike Protein
... teinase K digestion was examined as described ...to proteinase K digestion without binding to ...to proteinase K digestion as described for ...
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Evidence for antimicrobial activity associated with common house spider silk
... Our study of T. domestica silk shows that the anti- microbial activity against B. subtilis is reduced or negated after digestion with Proteinase K. The reduction of antimicrobial properties after treatment ...
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Appropriate coating methods and other conditions for enzyme-linked immunosorbent assay of smooth, rough, and neutral lipopolysaccharides of Pseudomonas aeruginosa.
... However, for smooth and neutral LPS prepared by the proteinase K-SDS method, the direct dehydration method was shown to be superior to the carbonate method (Fig. aeruginosa contains pred[r] ...
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Specificity of Rabbit Antisera against Lipopolysaccharide of Acinetobacter
... of proteinase K-digested whole-cell lysates were subjected to SDS-polyacrylamide gel electro- phoresis (PAGE) with a 5% stacking gel and a 10 or a 15% separating gel in the Laemmli ...
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Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay
... and proteinase K which were used to extract alpha 1 (II)-CB11B from the denatured (alpha-chymotrypsin soluble) and residual native (proteinase K soluble) collagen alpha-chains, respectively, ...
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Optimisation of DNA extraction from the crustacean Daphnia
... Three methods of homogenisation and lysis were evaluated: (i) a 2010 Geno/Grinder bead-based tissue homogeniser (SPEX SamplePrep, Stanmore, UK; 1.4 mm ceramic beads, 1,750 strokes/minute for 40 s); homogenisation was ...
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Probing the applicability of autotransporter based surface display with the EstA autotransporter of Pseudomonas stutzeri A15
... ase K assays and subsequent analysis of the samples with SDS-PAGE and Western blot using α-E-tag antibodies or α-EstA serum (Figure 2A, B and Additional file 4: Figure ...
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Chimeric hepatitis B virus core particles with parts or copies of the hepatitis C virus core protein.
... Susceptibility to digestion with proteinase K of HCV core determinants on chimeric core particlesa Conditions Undigested Digested 10 min 60 min A492 of antigenic determinants of: HCV cor[r] ...
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Monoclonal antibodies to surface-exposes proteins of Mycoplasma mycoides subsp. mycoides (small-colony strain), which causes contagious bovine pleuropneumonia.
... All of the MAbs made to disrupted whole M. mycoides subsp. mycoides organisms were IgM isotype and reacted with periodate-sensitive and proteinase K-insensitive epitopes, and seven caused in vitro growth ...
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Preparation of injectable hydrogel with near-infrared light response and photo-controlled drug release
... Photo-controlled release hydrogel provides a new strategy for treating tumours. Under the stimulation of external light sources, the ability to release the entrapped drug on time and space on demand has outstanding ...
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The URNA Genes of Herpesvirus Saimiri (Strain C488) Are Dispensable for Transformation of Human T Cells In Vitro
... In this study, all HSURs and the interspersed DHFR were precisely deleted from the strain C488 genome by a cosmid- based approach. First, a 6.6-kb fragment from a partial BamHI-NdeI digestion from the left terminal ...
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Bacteriophage phi29 terminal protein: its association with the 5' termini of the phi29 genome.
... For 429 DNA preparation, the disrupted phages were treated with proteinase K 200 jg/ml and extracted with phenol as de- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest [r] ...
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Analysis of the DNAs from seven varicella-zoster virus isolates.
... The VZV DNA was deproteinized by treatment with Proteinase K 100 Lg/ml for 2 h at 37°C, extracted with phenol saturated with TE buffer, and then extracted with 812 Downloaded from http:/[r] ...
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Synthesis, Characterization and Functionalization of Iron Oxide Magnetic Nanoparticles for Diagnostics and Therapy of Tumors
... Figure 7.1: Uptake measurement of MNPs by liver tissue calculated using different methods (“oil bath”, “oven”, with proteinase K and trypsin) based on ferrozine pro- tocol. Sample are: M[r] ...
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Simultaneous DNA and RNA isolation from brain punches for epigenetics
... SDS/Proteinase K method with a total yield of 850 ng per punch followed by the CTAB method (550 ng) and the DNeasy and Puregene isolation Kits (both 360 ...
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