PURIFICATION AND CHARACTERISATION
Purification and Characterisation of a Fungicidal Peptide from Bacillus amyloliquefaciens NCPSJ7
... By now, all reported antifungal peptides secreted by B. amyloliquefaciens were still under research in the laboratory. There is no available active antimi- crobial agent as a biopreservative for the pathogen infection. ...
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Purification and Characterisation of Lactate Dehydrogenase: An Undergraduate Biochemistry Laboratory Experiment
... the purification scheme starting from pig heart includes ammonium sulphate fractionation, desalting by size exclusion chromatography, anion exchange chromatography and pseudo-affinity ...different ...
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MUTATIONAL STUDY OF BACILLUS SPECIES FOR PRODUCTION, PURIFICATION AND CHARACTERISATION OF LIPASE
... complete purification percentage of yield was high for wild mutated as compare to ...wild. Characterisation of the lipase enzymes showed optimum pH ...
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Purification and characterisation of the bacteriocin produced by Lactobacillus plantarum, isolated from Chinese pickle
... Abstract Zhou F., Zhao H., Bai F., Piotr D., Liu Y., Zhang B. (2014): Purification and characterisation of the bacte- riocin produced by Lactobacillus plantarum, isolated from Chinese pickle. Czech J. Food ...
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The Purification and Characterisation of a Prolyl Oligopeptidase from the Cytosolic Fraction of Bovine Whole Brain
... Although an increase in slit widths would undoubtedly lead to an increase in sensitivity this would be accompanied by a parallel increase in inner filter effect interference. Although the quenching effect of bovine brain ...
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Purification and electrical characterisation of nanotube and nanowire polymer composites
... f purification and processability is to com bine nanotubes with a ...host, purification o f the as-produced nanotube m aterial is ...nanotube purification using complementary polymers along with ...
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Purification, characterisation and inhibition of methionine synthase from rat liver
... The main reason that the prokaryotic methionine synthase has been studied more extensively than the mammalian enzyme, even before it was cloned and expressed, is that there is significantly more methionine synthase per ...
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Purification and Characterisation of Xanthine Oxidoreductases from Local Bovids in Malta
... 4.2. Enzyme Activity The final product is a mixture of both XO and XDH. In the absence of a suitable reducing agent, the majority of XOR is normally converted to its oxidase form [20] [28]. In this study, DTT was ...
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Overexpression, extraction, purification and characterisation of DppA from Escherichia coli
... Fig. 5 (A) depicts the chromatographic profile of the purification of DppA utilizing ion exchange chromatography (IEX) procedure. IEX separates proteins on the basis of differences in their net surface charge (GE ...
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Isolation, Purification and Characterisation of Alpha amylase From Phaseolus Aconitifolius
... by Mg 2+ up to 101%, 1.5% substrate concentration (Starch solution) was found to be optimum, further increase in substrate concentration, the rate of reaction remains[r] ...
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Partial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
... (Matheis et al. 1984). PPO from different plant tissues shows different substrate specificities and degrees of inhibition. Therefore, characterisation of the enzyme could enable the development of more effective ...
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PARTIAL PURIFICATION AND CHARACTERISATION OF ACID PHOSPHATASES FROM DEVELOPING CHICKPEA SEEDS
... phosphatases, purification INTRODUCTION Phosphatases catalyse the hydrolysis of phosphate monoesters and are classified as acid and alkaline based solely upon their optimal activity at pH below or above 7 (Vincent ...
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Partial purification and characterisation of cytosolic Fructose-1, 6-bisphosphatase from Drymaria cordata
... Partial purification of the enzyme, fructose 1,6-bisphosphatase was carried out following the methods of homogenization, streptomycin sulphate precipitation, ammonium sulphate cut and molecular sieve ...
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Isolation and identification of a strain producing cold adapted β galactosidase, and purification and characterisation of the enzyme
... RESULTS Isolation and identification of cold-adapted β-galactosidase producing strain Isolate 14-1 was selected for further study because the cells grew at low temperatures and hydrolysed the chromogen X-gal which was ...
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Purification and characterisation of xylooligosaccharides (XOS) from wheat-based dried distillers grains with solubles
... ethanol (DDGS) which contains many other polysaccharides. First the starting materials should be water extracted. In order to remove the starch from the extract materials a treatment with α- amylase and amyloglucosidase ...
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STUDY ON PRODUCTION, PURIFICATION AND CHARACTERISATION OF L-ASPARAGINASE FROM ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA
... Water sample microbes were isolated and screened for the potential producers of L- asparaginase using modified M-9 medium plate method. The single discrete colonies which have exhibited clear pink zone surrounding ...
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Purification and Characterisation of Two Alpha-glucosidases From Termite Workers Macrotermes Bellicosus (Termitidae: Macrotermitinae)
... study, purification and characterization of two alpha-glucosidases from termite workers Macrotermes bellicosus (Termitidae: Macrotermitinae) were ...The purification procedure consisted of anion-exchange, ...
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Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.
... vestris (Morohoshi et al. 2012 ). The microflora associated with many plants of medicinal in- terest have been under-investigated, and this environment re- mains a rich and unexploited reservoir of microbes with biotech- ...
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PARTIAL PURIFICATION AND CHARACTERISATION OF COLD-ACTIVE METALLOPROTEASE BY BACILLUS SP. AP1 FROM APHARWAT PEAK, KASHMIR
... Effect of temperature on activity and stability of purified protease: Hydrolytic activity of purified enzyme was measured at varying temp- eratures to determine the ma[r] ...
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Isolation, screening, production, media optimization, partial purification and Characterisation of Cellulase producing Bacillus species isolated from Rhizosphere soil
... Bacillus species was isolated from rhizosphere soil and characterized by various staining procedures, biochemical analysis and partial purification. From my present study, the result showed that cellulase ...
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