Purine nucleoside phosphorylase
Inhibition and Structure of Toxoplasma gondii Purine Nucleoside Phosphorylase
... a purine auxotroph that relies on purine salvage for ...gondii purine nucleoside phosphorylase (TgPNP) stability and crystallized TgPNP with phosphate and immucillin-H, a ...
8
Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis
... Introduction Purine nucleoside phosphorylase (PNP) is involved in the purine- salvage ...of purine ribo- and deoxyribonucleosides and their ...the purine base and ...
5
Partial Purine Nucleoside Phosphorylase Deficiency: STUDIES OF LYMPHOCYTE FUNCTION
... J Clin Invest. 1978;61(4):1071-1080. https://doi.org/10.1172/JCI109006. Immune function in two brothers with a deficiency of purine nucleoside phosphorylase was evaluated in vivo and in vitro. Both ...
11
TAT mediated intracellular delivery of purine nucleoside phosphorylase corrects its deficiency in mice
... in purine nucleoside phosphorylase (PNP) enzyme activity result in abnormal nucleoside homeostasis, severe T cell immunodeficiency, neurological dysfunction, and early ...
11
Deoxyguanosine Triphosphate as a Possible Toxic Metabolite in the Immunodeficiency Associated with Purine Nucleoside Phosphorylase Deficiency
... https://doi.org/10.1172/JCI109058. Purine nucleoside phosphorylase (PNP) deficiency is associated with a severe defect in thymus-derived (T)-lymphocyte function combined with normal bone ...
6
Erythrocyte metabolism in purine nucleoside phosphorylase deficiency after enzyme replacement therapy by infusion of erythrocytes
... https://doi.org/10.1172/JCI109639. Purine nucleoside phosphorylase deficiency is associated with a severely defective T-cell ...with purine nucleoside phosphorylase deficiency ...
7
Proposed explanation for S adenosylhomocysteine hydrolase deficiency in purine nucleoside phosphorylase and hypoxanthine guanine phosphoribosyltransferase deficient patients
... with purine nucleoside phosphorylase deficiency had only 7% of control AdoHcyase activity, conforming the original ...the purine nucleosides known to accumulate in nucleoside ...
7
Propolis exerts an anti-inflammatory effect on PMA-differentiated THP-1 cells via inhibition of purine nucleoside phosphorylase
... Roifman, C.M.; Cohen, A. Mitochondrial Basis for Immune Deficiency: Evidence from Purine Nucleoside Phosphorylase–deficient Mice. J. Exp. Med. 2000, 191, 2197–2208. [CrossRef] 62. Herken, H.; Gurel, ...
22
Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT
... human purine nucleoside phosphorylase that is capable of utiliz- ing adenosine-based prodrugs as substrate ...human purine nucleoside phosphorylase (hPNP) cleaves 6-oxo purines ...
12
An unusual presentation of purine nucleoside phosphorylase deficiency mimicking systemic juvenile idiopathic arthritis complicated by macrophage activation syndrome
... Findings: We report a patient with a clinical presentation highly suggestive for systemic juvenile idiopathic arthritis (sJIA) onset complicated by MAS, and was later diagnosed with purine nucleoside ...
6
Targeted enzyme prodrug therapy for metastatic prostate cancer – a comparative study of L-methioninase, purine nucleoside phosphorylase, and cytosine deaminase
... Background: Enzyme prodrug therapy shows promise for the treatment of solid tumors, but current approaches lack effective/safe delivery strategies. To address this, we previously developed three enzyme-containing fusion ...
8
PURINE NUCLEOSIDE PHOSPHORYLASE ACTIVITY OF BLOOD I ERYTHROCYTES
... It is the purpose of this paper (a) to demon- strate the presence of PNP activity in erythro- cytes; (b) to indicate the comparative activity of PNP in the red blood cells of man, dog, r[r] ...
8
Fermentative production of ribavirin by overexpressing purine nucleoside phosphorylase in Bacillus
... product purine nucleoside was successfully converted to ...typical purine nucleoside-producing strains, guanosine-producing strain derivate was screened out as the optimal ribavirin-producing ...
8
Purine Nucleoside Phosphorylase Deficiency: EVIDENCE FOR MOLECULAR HETEROGENEITY IN TWO FAMILIES WITH ENZYME DEFICIENT MEMBERS
... The mother of family B displays a normal starch gel iso- zyme pattern of reduced activity, and a variant protein concentration of about 11% of her total NP, suggesting. an unstable varia[r] ...
7
Propolis exerts an anti-inflammatory effect on PMA-differentiated THP-1 cells via inhibition of purine nucleoside phosphorylase
... Further study using a metabolic approach identified metabolic alterations that may contribute to the subsequent anti-inflammatory events. Both LPS and propolis changed the levels of metabolites in several different ...
21
Decreased methionine synthesis in purine nucleoside treated T and B lymphoblasts and reversal by homocysteine
... https://doi.org/10.1172/JCI111536. Purine nucleosides, which accumulate in adenosine deaminase and purine nucleoside phosphorylase deficiency, are toxic to lymphoid ...
8
Diverse models for anti-HIV activity of purine nucleoside analogs
... Conclusions Diverse techniques such as DT, RF, SVM, and MAA were successfully used to develop models for anti-HIV purine nucleoside analogs. Models based on DT, RF, and SVM statistical approaches show an ...
10
Lethal Mutagenesis of Picornaviruses with N-6-Modified Purine Nucleoside Analogues
... with these analogues was able to increase the frequency of transition mutations by more than 50-fold over a single pas- sage, as measured by a phenotypic assay, suggesting a role of lethal mutagenesis in the observed ...
10
Purine nucleoside metabolism in the erythrocytes of patients with adenosine deaminase deficiency and severe combined immunodeficiency
... undetectable in affected patients (homozygotes), and approximately one-half normal levels are found in individuals heterozygous for ADA deficiency. The metabolism of purine nucleosides was studied in erythrocytes ...
12
Structure of E. coli 5′-methylthioadenosine/S-adenosylhomocysteine Nucleosidase Reveals Similarity to the Purine Nucleoside Phosphorylases
... the inosine substrate and the O4 phosphate, favors the since the enzyme is found in many microbes but does migration of electrons toward the adenine base and the not exist in mammalian cells. Selective inhibition of the ...
13