Pyruvate decarboxylase
A potential energy profile of the catalytic cycle of pyruvate decarboxylase
... Pyruvate decarboxylase (PDC, EC 4.1.1.1) is a 240 kDa protein [1] that belongs to the lyase carbon-carbon class of enzymes. Its catalytic activity is essential for several metabolic pathways in both ...
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Starmerella bombicola influences the metabolism of Saccharomyces cerevisiae at pyruvate decarboxylase and alcohol dehydrogenase level during mixed wine fermentation
... Background: The use of a multistarter fermentation process with Saccharomyces cerevisiae and non-Saccharomyces wine yeasts has been proposed to simulate natural must fermentation and to confer greater complexity and ...
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Role of phosphate limitation and pyruvate decarboxylase in rewiring of the metabolic network for increasing flux towards isoprenoid pathway in a TATA binding protein mutant of Saccharomyces cerevisiae
... of pyruvate-acetaldehyde-acetate cycle in the cytoplasm due to high flux in the reaction caused by pyruvate decarboxylase ...and pyruvate decarboxylase 6 ...
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Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities
... industries. Pyruvate decarboxylase (Pdc)‑deficient Saccharomyces cerevisiae is an attractive host strain for pro‑ ducing 2,3‑BD because a large amount of pyruvate could be shunted to 2,3‑BD ...
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An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae
... of pyruvate decarboxylase, which catalyzes the first step in the conversion of pyruvate to ...encoding pyruvate decarboxylase (PDC1, PDC5 and PDC6 [27]) were deleted, did not produce ...
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Histone H1 Is Required for Proper Regulation of Pyruvate Decarboxylase Gene Expression in Neurospora crassa
... The slow linear growth phenotype of N. crassa hH1 mutants was dependent on the carbon source, suggesting that histone H1 may play a role in pathways associated with carbohydrate metabolism. Based on the linear growth of ...
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Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase
... An alternative pathway for ethanol production involves direct conversion of pyruvate to acetaldehyde by the enzyme pyruvate decarboxylase (PDC). This pathway is simpler than the PFOR pathway because ...
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Suppression of pdc2 Regulating Pyruvate Decarboxylase Synthesis in Yeast
... binds to the upstream region of the pyruvate decarboxylase struc- tural gene PDCl of Saccharomyces cerevisiae. FRAENKEI., 1982 Mutant studies of yeast phos- phofructokinase. FR[r] ...
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A defect in pyruvate decarboxylase in a child with an intermittent movement disorder
... decarboxylase in the father's sonicated fibroblasts were intermediate between those of the patient and those of controls. Values for the mother were at or just below the lower limits of the ranges in controls. ...
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Studies on pyruvate decarboxylase catalysed acyloin formation and the effects of surfactants on lipase catalysed hydrolysis of esters
... A Thesis Submitted for the Degree of PhD at the University of Warwick Permanent WRAP URL: http://wrap.warwick.ac.uk/110304 Copyright and reuse: This thesis is made available online and i[r] ...
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Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain
... Results: In this study, a Pdc negative strain was adaptively evolved for improved growth in glucose medium via serial transfer, resulting in three independently evolved strains, which[r] ...
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Manipulation of the levels of pyruvate decarboxylase and alcohol dehydrogenase for submergence tolerance in rice
... Similar levels of anoxia tolerance were found in mature plants of the line which over-produced ADH and WT whereas in the flooding tolerant variety, anoxia tolerance was much higher.. Thi[r] ...
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Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae
... encode pyruvate decarboxylase enzymes with a preference for linear-chain 2-oxo acids (pyruvate, 2-oxobutanoate and 2-oxopentanoate) ...for pyruvate [13] renders them unsuitable for ...
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Acidosis (Measured by Nuclear Magnetic Resonance) and Ethanol Production in Anoxic Goldfish Acclimated to 5 and 20°C
... This phenomenon could, in theory, be based on three different processes: 1 activation of the ethanol pathway, causing removal of lactate by lactate dehydrogenase, pyruvate decarboxylase [r] ...
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Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities
... Metabolic engineering strategies have been used to mod- ify microorganisms to convert all sugars arising from chemical-enzymatic hydrolysis of lignocellulose, such as xylose, arabinose, and glucose into ethanol. A wide ...
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Molecular mimicry in primary biliary cirrhosis Evidence for biliary epithelial expression of a molecule cross reactive with pyruvate dehydrogenase complex E2
... * Antibodies to pyruvate decarboxylase stain specimens only in a typical mitochondrial pattern without evidence of staining at the luminal surface; * number of positive/total; § typical [r] ...
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Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis
... through pyruvate carboxylase, the fermentative pathways and malic enzyme, a flux decrease through the TCA cycle, and a partial relocation of alanine biosynthesis from the mitochondrion to the ...of pyruvate ...
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Efficient production and secretion of oxaloacetate from Halomonas sp. KM-1 under aerobic conditions
... of pyruvate under aerobic ...and pyruvate secretion in the KM-1 strain and, unexpectedly, observed that oxaloacetate, an important intermediate chemical in the TCA cycle, glycogenesis, and aspartic acid ...
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Mitochondrial physiology and reactive oxygen species production are altered by hypoxia acclimation in killifish (Fundulus heteroclitus)
... mass:volume fatty-acid free bovine serum albumin; pH 7.4 when measured at 20°C). The tissue was gently homogenized on ice with six passes of a loose-fitting Potter-Elvehjem homogenizer at 30 r.p.m. Homogenate was ...
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Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance
... the mitochondria was activated by the overexpression of Ilv2p (Figure 4a). The isobutanol production in the con- trol strain BSW13 (23 ± 3 mg/L, YPH499/pATP426- kivd-ADH6/pATP425/pATP423) is lower than that of BSW4 (45 ± ...
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