Pyruvate Dehydrogenase Complex
Engineering Acetyl Coenzyme A Supply: Functional Expression of a Bacterial Pyruvate Dehydrogenase Complex in the Cytosol of Saccharomyces cerevisiae
... Efficient growth and metabolism of Acs ⴚ S. cerevisiae through cytosolic expression of E. faecalis PDH. Replacement of the native route to cytosolic acetyl-CoA might have substantial effects on cellular physiology, by ...
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Pyruvate Dehydrogenase Complex Activity in Normal and Deficient Fibroblasts
... Pyruvate dehydrogenase complex (PDC) activity in human skin fibroblasts appears to be regulated by a phosphorylation-dephosphorylation mechanism, as is the case with other animal ...of ...
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Defects in the E2 lipoyl transacetylase and the X lipoyl containing component of the pyruvate dehydrogenase complex in patients with lactic acidemia
... missing X component while the other had two distinct bands where X should be on Western blotting of fibroblast proteins. All three patients appeared to have severe clinical sequelae resulting from these defects. This is ...
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Carbohydrate Metabolism in the Toxoplasma gondii Apicoplast: Localization of Three Glycolytic Isoenzymes, the Single Pyruvate Dehydrogenase Complex, and a Plastid Phosphate Translocator
... to pyruvate kinase, are present in the ...and pyruvate kinase were found to localize to the ...single pyruvate dehydrogenase complex, which was located in the apicoplast and absent in ...
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Central Hypoventilation Syndrome in Pyruvate Dehydrogenase Complex Deficiency
... 74:1034-1040; central hypoventilation syndrome, doxap- ram, almitrine, lactic acidosis, pyruvate dehydrogenase complex.. In recent years, more than 50 patients have been reported with va[r] ...
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Deficiency of the pyruvate dehydrogenase component in pyruvate dehydrogenase complex deficient human fibroblasts Immunological identification
... of pyruvate dehydrogenase complex were analyzed using an immunoblot technique and specific ...the pyruvate dehydrogenase complex activity was due to a reduction in the amount of ...
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Defect in the X-Lipoyl-Containing Component of the Pyruvate Dehydrogenase Complex in a Patient With a Neonatal Lactic Acidemia
... Immunoreactive pro- teins were visualized by electrogenerated chemiluminescence de- tection reagents: panel A, (lane 1) purified pig heart PDH complex (2.5 g protein); (lane 2) control c[r] ...
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Molecular mimicry in primary biliary cirrhosis Evidence for biliary epithelial expression of a molecule cross reactive with pyruvate dehydrogenase complex E2
... * Antibodies to pyruvate decarboxylase stain specimens only in a typical mitochondrial pattern without evidence of staining at the luminal surface; * number of positive/total; § typical [r] ...
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Engineering of Serine-Deamination pathway, Entner-Doudoroff pathway and pyruvate dehydrogenase complex to improve poly(3-hydroxybutyrate) production in Escherichia coli
... It is reasonable that efficient conversion of L-serine to pyruvate requires sufficient availability of L-serine. To enhance the biosynthesis of L-serine, we overexpressed the genes of de novo L-serine biosynthetic ...
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Pyruvate dehydrogenase complex stimulation promotes immunometabolic homeostasis and sepsis survival
... Over 15 million deaths worldwide are due sepsis (31), with 90-day mortality rates for patients in septic shock approaching 50% (32). Sepsis incurs the greatest in-hospital cost of any illness and is responsible for most ...
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Pyruvate Dehydrogenase Complex Deficiency as a Cause of Subacute Necrotizing Encephalopathy (Leigh Disease)
... These findings, considered together with the aggregate data in the liter- ature, suggest strongly that pyruvate dehydrogenase corn- plex deficiency is the basic defect in a subgroup of p[r] ...
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The pivotal role of pyruvate dehydrogenase kinases in metabolic flexibility
... the pyruvate dehydrogenase complex ...by pyruvate dehydrogenase (PDH) kinase (PDK) is crucial to maintain energy homeostasis under some extreme nutritional conditions in ...
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Rearrangement of mitochondrial pyruvate dehydrogenase subunit dihydrolipoamide dehydrogenase protein–protein interactions by the MDM2 ligand nutlin-3
... Dihydrolipoamide dehydrogenase, the E3 subunit of the mitochondrial pyruvate dehydrogenase complex, was one of two Nutlin-3 perturbed proteins identified fours hour posttreatment at two cell ...
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Phosphorylation of the PDH complex precedes HIF-1-mediated effects and PDK1 upregulation during the first hours of hypoxic treatment in HCC cells
... The pyruvate dehydrogenase complex (PDC) is a large ...multienzyme complex that is localized in the mitochondrial ...of pyruvate, the end product of glycolysis, to acetyl coenzyme A ...
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Metabolic plasticity maintains proliferation in pyruvate dehydrogenase deficient cells
... The pyruvate dehydrogenase complex (PDH) occupies a crucial node in glucose metabolism, as it oxidatively decarboxylates pyruvate generated from glycolysis or other pathways to generate ...
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Biochemical and ultrastructural study of Blastocystis hominis
... Despite the presence of large numbers of mitochondria, activities of the mitochondrial enzymes pyruvate dehydrogenase complex, a-ketoglutarate dehydrogenase complex, isocitrate dehydroge[r] ...
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Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance
... the pyruvate dehydrogenase complex such as LPD1, indicating that the pyruvate supply for isobutanol biosynthesis is competing with acetyl-CoA biosynthesis in ...as pyruvate carboxylase, ...
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Molecular docking studies of (4Z, 12Z) cyclopentadeca 4, 12 dienone from Grewia hirsuta with some targets related to type 2 diabetes
... [17]. Pyruvate dehydrogenase kinase isoforms (PDKs 1 - 4) (PDB ID 4MP2) negatively regulate activity of the mitochondrial pyruvate dehydrogenase complex (PDC) by reversible ...
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The compartmentation of nitrogenous assimilates in suspension cultured cells of carrot
... properties of the pea chloroplast pyruvate dehydrogenase complex.. Hydrolysis of Intracellular proteins in vacuoles isolated from Acer pseudoplatanus cells. Partitioning of nitroge[r] ...
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Effects of insulin infusion on human skeletal muscle pyruvate dehydrogenase, phosphofructokinase, and glycogen synthase Evidence for their role in oxidative and nonoxidative glucose metabolism
... or pyruvate dehydrogenase (PDH) in skeletal muscle regulates intracellular glucose metabolism, subjects were studied basally and during euglycemic insulin infusions of 12, 30, and 240 mU/m2 X ...
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