pyruvate kinase M2
Pyruvate Kinase M2 Regulates Hif 1alfa Activity and IL 1ß Induction and Is a Critical Determinant of the Warburg Effect in LPS Activated Macrophages
... Macrophages activated by the TLR4 agonist LPS un- dergo dramatic changes in their metabolic activity. We here show that LPS induces expression of the key metabolic regulator Pyruvate Kinase M2 ...
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Original Article Expression of pyruvate kinase M2 in human colorectal cancer and its prognostic value
... little pyruvate is dis- patched to mitochondria for oxidative phos- phorylation even in the presence of sufficient ...oxygen. Pyruvate kinase M2 (PKM2), a key ...
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Small ubiquitin-like modifier 1 modification of pyruvate kinase M2 promotes aerobic glycolysis and cell proliferation in A549 human lung cancer cells
... 0.73±0.11, P=0.032). (D) A549 cells were transfected with HA-tagged PKM2-WT or K336R. After 24 h of transfection, the cells were replated into appropriate plates for analysis of basal oxygen consumption rate by a ...
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Original Article Both high expression of pyruvate kinase M2 and vascular endothelial growth factor-C predicts poorer prognosis in human breast cancer
... Abstract: Pyruvate kinase M2 (PKM2) and vascular endothelial growth factor-C (VEGF-C) have been known to play an important role in tumorigenesis and tumor progression in breast ...
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Original Article Aconitate 2 (ACO2) and pyruvate kinase M2 (PKM2) are good predictors of human sperm freezability
... and pyruvate kinase M2 (PKM2) are closely related to glucose metabo- lism and our previous study showed these two proteins had marked differences between the fresh and freeze-thawed sperm samples ...
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<p>Phosphoribosyl Pyrophosphate Amidotransferase Promotes the Progression of Thyroid Cancer via Regulating Pyruvate Kinase M2</p>
... activating pyruvate kinase (PK) and can function as a biomarker for aggressive lung ...duce pyruvate and release ...them, pyruvate kinase M2 (PKM2) is reported to be a key ...
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Targeting stromal-induced pyruvate kinase M2 nuclear translocation impairs OXPHOS and prostate cancer metastatic spread
... Cancer associated fibroblasts (CAFs) are key determinants of cancer progression. In prostate carcinoma (PCa), CAFs induce epithelial-mesenchymal transition (EMT) and metabolic reprogramming of PCa cells towards oxidative ...
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Pyruvate kinase M2 prevents apoptosis via modulating Bim stability and associates with poor outcome in hepatocellular carcinoma
... Pyruvate kinase M2 (PKM2) contributes to the Warburg effect, a hallmark of cancer. We showed that PKM2 levels were correlated with overall survival (hazard ration = 1.675, 95% confidence interval: ...
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Leptin promotes epithelial-mesenchymal transition of breast cancer via the upregulation of pyruvate kinase M2
... that leptin could promote EMT in breast cancer cells [20], and IL-8 was one of the key molecules which affected leptin-mediated EMT. To explore other key regulatory molecules that are involved in leptin-induced EMT of ...
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Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to the development of pulmonary arterial hypertension
... ATP. Pyruvate kinase catalyzes the final step in glycolysis by transferring the phosphate from phosphoenolpyruvate (PEP) to ADP, thereby generating pyruvate and ATP ...The M2 isoform of ...
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Expression of pyruvate kinase M2 in human bladder cancer and its correlation with clinical parameters and prognosis
... PKM2, pyruvate kinase M2; UcB, urothelial carcinoma of the bladder; h&e, hematoxylin and eosin; ihc, immunohistochemistry; Pcr, polymerase chain reaction; RQ, relative quantification; c, bladder ...
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Pyruvate kinase M2 is requisite for Th1 and Th17 differentiation
... Co-IP. After Flag-tagged CaMK4/6×His-tagged PKM2 cotransfection, cells were harvested in 1% NP40 lysis buffer. Co-IP was performed with the Dynabeads Protein G Immunoprecipitation Kit (Life Technol- ogies) according to ...
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Pyruvate kinase M2 regulates glucose metabolism by functioning as a coactivator for hypoxia-inducible factor 1 in cancer cells
... xenograft growth in nude mice [3]. The binding of tyrosine- phosphorylated peptides to PKM2 at lysine-433 was found to inhibit PKM2 enzymatic activity through release of the allosteric activator fructose-1,6-bisphosphate ...
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Endothelial pyruvate kinase M2 maintains vascular integrity
... Our data uncover novel dual roles for PKM2 in proliferating and quiescent ECs. Proliferating ECs, though primary cells, display a number of features of cancer cells, including capacity for rapid pro- liferation, ...
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A systematic review and meta analysis of the diagnostic accuracy of pyruvate kinase M2 isoenzymatic assay in diagnosing colorectal cancer
... Stool-based pyruvate kinase isoenzyme M2 (M2-PK) is one such biomarker under ...of M2-PK as a screening tool in colorectal ...for M2-PK to be 79% (CI 73% – 83%) and 80% (CI 73% – ...
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<p>Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells</p>
... Protein expression levels were examined by Western blotting. 22 Total protein was separated from cells or tissues using RIPA lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China) supplemented with ...
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Pyruvate kinase M2 and the mitochondrial ATPase Inhibitory Factor 1 provide novel biomarkers of dermatomyositis: a metabolic link to oncogenesis
... 27]. Pyruvate kinase (PK) is the enzyme that catalyzes the final step in glycolysis, converting phos- phoenolpyruvate (PEP) to pyruvate ...of pyruvate kinase exist: type-R and type-L ...
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Pyruvate kinase M2 overexpression and poor prognosis in solid tumors of digestive system: evidence from 16 cohort studies
... The effect of PKM2 expression on the clinicopathological features in different digestive tumors was further analyzed and listed in Table 2. When focusing on GC and biliary cancer, a s[r] ...
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Nonmetabolic functions of metabolic enzymes in cancer development
... as pyruvate kinase M2 (PKM2), phosphoglycerate kinase 1 (PGK1), ketohexokinase-A (KHK-A), hexokinases (HK), nucleoside diphosphate kinase (NDPK or NDK), and ...
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The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation
... Our interactome analysis has revealed that TG2 interacts with various proteins belonging to different functional categories such as chaperones, mitochondria, metabolism and cytoskeleton (Figure S2). Particularly, out of ...
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