qPCR assay
Semi-Quantitative, Duplexed qPCR Assay for the Detection of Leishmania spp. Using Bisulphite Conversion Technology
... Probe-based qPCR can overcome these ...diagnostic assay, based on bisulphite conversion and real-time PCR technologies, a series of experiments were performed to assess the limit of detection and ...
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Development of a real time QPCR assay for the detection of RV2 lineage specific rhadinoviruses in macaques and baboons
... RV2 QPCR assay, DNA obtained from lymphocytes of baboon Pcy78404 was tested for the presence of a related RV2 rhadinovirus species under the standard assay ...
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The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling
... any assay interference was caused by the presence of (residual) ENMs in the genetic ...no assay verification was reported in this ...no assay verification was reported in this ...reported ...
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The EpiTect Methyl qPCR Assay as novel age estimation method in forensic biology
... Methylation changes at specific gene regulatory regions can provide insights to define contributor age for criminal[5]. Researchers have found that 60–70% of promoter regions of the human genome overlap with CpG islands ...
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Development of a Real-Time qPCR Assay for Quantification of Covert Baculovirus Infections in a Major African Crop Pest
... real-time qPCR technique allowing the assessment of covert baculovirus infections within both larval and adult forms of the host ...the qPCR assay was used to: (i) quantify the dynamics of covert ...
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Sample ready multiplex qPCR assay for detection of malaria
... interpret qPCR assays ...multiplex qPCR assay for detection of Plasmodium genus target, and species specific Plasmodium falciparum , Plasmodium vivax targets as well as human RNaseP gene as an ...
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Development of a fast and low-cost qPCR assay for diagnosis of acute gas pharyngitis
... by qPCR were treated with antibiotics effective against GAS, even though the health care providers responsi- ble for these patients were unaware of the qPCR ...the qPCR method versus the results of ...
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Development and application of an AllGlo probe based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis
... fluorophore at the 5’ end and a nonfluorescent quencher at the 3’ end, AllGlo probes have two identical reporter dyes that normally quench themselves. Upon hybridization with the target sequence, the labelled oligo ...
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SPUD qPCR Assay Confirms PREXCEL-Q Software's Ability to Avoid qPCR Inhibition
... In this study, we set out to examine the presence or absence of inhibition in sheep lung total RNA isolates subjected to one- step qPCR (using Invitrogen’s SuperScript™ III Platinum ® One-Step quantitative RT-PCR ...
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Group B Streptococcus detection in pregnant women: comparison of qPCR assay, culture, and the Xpert GBS rapid test
... The overall prevalence of GBS colonization varies de- pending on the studied population and the test used for screening. This variability may be related to various cli- matic, biological, sociocultural, geographic, and ...
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An eDNA qPCR assay to detect the presence of the parasite Schistocephalus solidus inside its threespine stickleback host
... Detecting the presence of a parasite within its host is crucial to the study of host – parasite interactions. The Schistocephalus solidus – threespine stickleback pair has been studied extensively to investigate host ...
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One-Step RT-qPCR assay for detection and quantification of equine infectious anemia virus in-vitro
... Increasingly, One-Step RT-qPCR assays have been standardized for the detection and early diagnosis of viral diseases, as in the study by Bao et al. (2008) with Pest des petits ruminants virus; their system was ...
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Comparison of qPCR and culture methods for group B Streptococcus colonization detection in pregnant women: evaluation of a new qPCR assay
... by qPCR, although the samples were not checked by another technique to resolve discrepan- ...two qPCR methods used in this study were based on different target genes, so non-specific amplification was ...
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Identification of human intestinal parasites affecting an asymptomatic peri urban Argentinian population using multi parallel quantitative real time polymerase chain reaction
... multiplex qPCR have the advantage of enabling detection of mul- tiple parasite species using a single stool sample as well as the ability to determine the intensity of infection of each species [12, ...parallel ...
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A real time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
... each assay. The limit of detection for both the rt-qPCR hydrolysis probe assay and SYBR Green rt- qPCR is, ...Green assay but not in the hydrolysis probe ...Green assay may ...
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Rapid Identification of Measles Virus Vaccine Genotype by Real Time PCR
... new assay was able to detect RNA from five currently used vaccine strains, AIK-C, CAM-70, Edmonston- Zagreb, Moraten, and ...RT-qPCR assay has been used suc- cessfully for measles surveillance in ...
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Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green Based Quantitative PCR Assay
... green qPCR performed well in comparison with the established culture method, indicating that it could be an effective rapid alternative to culture for the identification of ...green qPCR shares many ...
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A qPCR and multiplex pyrosequencing assay combined with automated data processing for rapid and unambiguous detection of ESBL-producers Enterobacteriaceae
... Another interesting case was R080 (K. pneumoniae) isolate. Though clearly displaying an ESBL-phenotype, this isolate was lacking the three antibiotic resistance determinant genes as targeted by our qPCR ...
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Detecting Genetic Mosaicism in Cultures of Human Pluripotent Stem Cells.
... the qPCR assay of around 10% for copy- number changes allows genetic variants to be detected before they overtake the cultures and mosaic cultures can be either discarded or ...by qPCR even when ...
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Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV like sequences
... TaqMan qPCR assay specific for XMRV pol sequences was able to detect viral DNA from 2 XMRV-infected cells (~ 10-12 pg DNA) in up to 5 μg of human genomic DNA, but yielded negative results in the test of 600 ...
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