Quantitative PCR (qPCR)
Quantitative PCR (qPCR) Detection of Mucorales DNA in Bronchoalveolar Lavage Fluid To Diagnose Pulmonary Mucormycosis
... targeted PCR in clinical samples can provide early and accurate information about the etiological ...that quantitative PCR (qPCR) detection of Mucorales DNA in serum could anticipate the ...
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Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR based mutation scanning approach
... genus. PCR-based tools employing various nuclear ...a quantitative PCR (qPCR), combined with single-strand conformation polymorph- ism (SSCP)-based sequencing analysis, was used for the ...
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Development of quantitative PCR for the detection of Alkalilimnicola ehrlichii, Thioalkalivibrio sulfidiphilus and Thioalkalibacter halophilus in gas biodesulfurization processes
... developed quantitative PCR (qPCR) assays to quantify haloalkaliphi‑ lic sulfur‑oxidizing gammaproteobacterial species Alkalilimnicola ehrlichii, Thioalkalivibrio sulfidiphilus, and Thioalkali- bacter ...
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Quantitative PCR Assessments of Bacterial Species in Women with and without Bacterial Vaginosis
... broad-range PCR surveys, which show that there are scores of species and genera com- mon to the vaginal environment that had not been recognized, and many of these represent new, as-yet-uncultivated species (18, ...
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Comparison of Sputum and Nasopharyngeal Aspirate Samples and of the PCR Gene Targets lytA and Spn9802 for Quantitative PCR for Rapid Detection of Pneumococcal Pneumonia
... sputum qPCR results, NpA qPCR results showed lower DNA levels and more-scattered patterns of quanti- tative results in cases with pneumococcal etiology ...between quantitative results of sputum ...
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MUCOXIN ENHANCED TRANSCRIPTION AND PROTEIN EXPRESSION OF P53 IN BREAST CANCER CELL LINE T47D
... However, the molecular mechanisms of mucoxin in inducing apoptosis and inhibiting proliferation has not fully understood. One of the genes that play a role in the apoptosis process and prolife- ration is the p53 gene ...
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Detection and Differentiation of Leishmania spp in Clinical Specimens by Use of a SYBR Green Based Real Time PCR Assay
... conventional PCR method that amplifies the rRNA internal transcribed spacer 2 (ITS2) region (ITS2-PCR), followed by DNA sequencing analysis of the amplicons to identify the species ...real-time ...
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A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants
... transcriptase quantitative–PCR (RT–qPCR), we confirmed that the decrease in VENUS fluorescence after wounding was not due to an altered expression of the receptor COI1 and of the sensor Jas9-VENUS, as ...
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Quantitative Detection of Borrelia burgdorferi in 2 Millimeter Skin Samples of Erythema Migrans Lesions: Correlation of Results with Clinical and Laboratory Findings
... Lyme borreliosis, the most common vector-borne disease of the Northern Hemisphere, is caused by a group of spirochetes referred to as Borrelia burgdorferi sensu lato. Humans become infected with these organisms during ...
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Bacterial Lymphadenitis at a Major Referral Hospital in France from 2008 to 2012
... and quantitative PCR (qPCR) was significantly better than 16S rRNA gene PCR (rrs) for the detection of Bartonella henselae (P ⴝ ...rrs PCR does not exclude the diagnosis of infectious ...
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A qPCR method to quantify bioavailable phosphorus using indigenous aquatic species
... gradient PCR were performed before the quantitative ...the PCR products were cloned into PMD19-T vec- tors, and sequenced at Generay ...China). PCR product was analyzed on a 0.2% agarose gel. ...
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Phytase producing Bacillus sp inoculation increases phosphorus availability in cattle manure
... improve Po hydrolysis in manure with a concomitant increase in P availability for plants requires further evaluation. The primary concerns regarding the use of these bacteria as manure inoculants are the inoculum ...
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Metagenomic Approach for Identification of the Pathogens Associated with Diarrhea in Stool Specimens
... difficile PCR (Cepheid, Sunnyvale, ...performed quantitative PCR (qPCR) to evaluate the abundance of ...real-time PCR was performed using the 7500 Fast real-time PCR system ...
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Retroviral activation of the mir 106a microRNA cistron in T lymphoma
... used quantitative PCR (qPCR) to measure expression lev- els of the primary transcript (Kis2) and the mature miR- NAs (mmu-mir-106a and mmu-mir-363) in tumors containing mir-106a cistron integrations ...
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Absolute Quantification of Individual Biomass Concentrations in a Methanogenic Coculture
... a quantitative PCR (qPCR) approach for the direct determination of individual biomass concentrations in methanogenic microbial associations by correlating the native qPCR signal (cycle ...
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Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice
... Real-time quantitative PCR RT-qPCR analysis of myeloperoxidase MPO, a marker for activated neutrophils 38, confirmed the defective neutrophilic inflammation in Lxra–/–Lxrb–/– and Lxra–/–[r] ...
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Molecular Studies Neglect Apparently Gram Negative Populations in the Human Gut Microbiota
... We are confident in our results because flow cytometry is a rapid and reliable method to quantify bacteria in fecal samples (39, 40, 53), and Gram staining is a technique that is commonly used in clinical bacteriology to ...
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Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... real-time PCR (RT-qPCR) or the success of genotyping of MeV from samples stored on FTA cards compared to that for TS or oral fluid (OF) samples that were collected and transported using the reverse cold ...
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Aspergillus Specific Lateral Flow Device and Real Time PCR Testing of Bronchoalveolar Lavage Fluid: a Combination Biomarker Approach for Clinical Diagnosis of Invasive Pulmonary Aspergillosis
... The EORTC/MSG criteria (5, 6) are often used as a diagnostic reference standard because of the rarity of proven IPA cases. How- ever, misclassification by the use of these criteria can occur for many reasons; e.g., lung ...
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Unbiased Detection of Respiratory Viruses by Use of RNA Sequencing Based Metagenomics: a Systematic Comparison to a Commercial PCR Panel
... resistance or pathogenicity. In our study, molecular typing was possible for 84% of all viral strains. Relevant information derived from typing included the following: (i) almost two-thirds of rhi- noviruses belonged to ...
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