Quantitative polymerase chain reaction (PCR)
Single-base resolution of mouse offspring brain methylome reveals epigenome modifications caused by gestational folic acid
... genome from the HMFA group (Additional file 2: Table S2, Additional file 3: Table S3, Additional file 9: Table S4, Additional file 4: Table S5, and Additional file 5: Table S6). For example, zinc finger proteins Zfp608 ...
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Seven-microRNA panel for lung adenocarcinoma early diagnosis in patients presenting with ground-glass nodules
... Methods: To identify potential miRNAs of interest, we analyzed the miRNA expression profile of tumor and adjacent non-para-tumor tissue in three participants by next-generation sequencing (NGS). We then assessed the ...
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Circulating miRNAs reflect early myocardial injury and recovery after heart transplantation
... cTnI: Cardiac troponin I; miRNAs: microRNAs; mRNAs: messenger RNAs; AMI: Acute myocardial infarction; qRT-PCR: quantitative reverse transcription polymerase chain reaction; SD: Standard [r] ...
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Original Article Serum microRNA-21 is a potential diagnostic marker for earlier lung squamous cell carcinoma detection
... LSCC. Quantitative reverse transcription-polymerase chain reaction (QRT-PCR) was used to measure the expression of miR-21 in serum of 50 patents with early-stage LSCC, 50 patients with ...
7
The expression and prognostic value of protein tyrosine kinase 6 in early-stage cervical squamous cell cancer
... Ten fresh cervical squamous cell cancer specimens (from 6 patients with FIGO stage IB1 tumor and 4 patients with FIGO stage IIA1 tumor; 2, 3, and 5 of the 10 patients had grade 3, 2, and 1 tumors, respectively) and their ...
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Molecular biology techniques and applications for ocean sensing
... the polymerase chain reaction (PCR) and reverse- transcriptase PCR, quantitative PCR, whole assemblage “fin- gerprinting” approaches (based on nucleic acid sequence or ...
13
Clinicopathological and prognostic significance of long noncoding RNA MALAT1 in human cancers: a review and meta-analysis
... qRT-PCR: quantitative real-time polymerase chain reaction; ISH: in situ hybridization; HWE: hardy–weinberg equilibrium; HR: hazard ratio; OS: overall survival; DFS: disease-free ...
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miR-486-5p suppresses prostate cancer metastasis by targeting Snail and regulating epithelial–mesenchymal transition
... C4-2. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) results demon- strated that the metastatic C-4 cell line showed significantly decreased expression ...
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Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... USA), PCR primer from Nanjing Plant Industry Company, ultracentrifuge (BIOSOURCE, USA), gel imaging system (Shanghai Shida Biotechnological Company), Ficoll-Hypaque solution (Sigma-Aldrich), Eppendorf tubes ...
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The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer
... Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were performed to determine the messenger RNA and protein expression levels of Vimentin, ...
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Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion
... digital polymerase chain reaction (ddPCR) has been shown to be advantageous compared to real-time quantitative PCR due to its in- creased accuracy, reproducibility, and sensitivity ...
14
PubMedCentral-PMC4931989.pdf
... performed quantitative real-time polymerase chain reaction (PCR) with these upregulated miRNAs from the miRNA microarray, and 8 miRNAs showed significantly increased expression in A2780 ...
19
Upregulation of inflammatory genes in the nasal mucosa of patients undergoing endonasal dacryocystorhinostomy
... controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed for the inflammatory genes interleukin (IL)-6, IL-1β, and CCL2, and because of a clear ...
7
Analysis of striatal transcriptome in mice overexpressing human wild-type alpha-synuclein supports synaptic dysfunction and suggests mechanisms of neuroprotection for striatal neurons
... 0.2 mM NaVa, 10 U/ml aprotinine, 20 U/ml DNAse I, 2 ug/ml RNAse, 1% mercaptoethanol) and centrifuged for 2 min, at 12,000 × g, at 4°C; and the soluble fraction was collected and frozen until used. The insoluble pellet ...
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GCN5 inhibits XBP-1S-mediated transcription by antagonizing PCAF action
... HEK293, 293T, and MCF7 cells were obtained from American Type Culture Collection. EBV- infected NPC-TW01 cells were described in our previous study [35]. The GCN5 siRNAs (siGCN5-3: 5’-CTCCATTTGAGAAACCTAATA-3’ and ...
17
Identification and characterization of a novel efficient resistance response to the furoviruses SBWMV and SBCMV in barley
... Previous studies suggested that P. graminis ribotype I may preferentially infect barley and transmit bymoviruses such as Barley yellow mosaic virus and Barley mild mosaic virus, whereas P. graminis ribotype II may ...
13
Transcriptional targeting of sphingosine-1- phosphate receptor S1P2 by epigallocatechin- 3-gallate prevents sphingosine-1-phosphate- mediated signaling in macrophage-differentiated HL-60 promyelomonocytic leukemia cells
... Notes: serum-starved hl-60 cells were treated for 18 hours with 3 µM PMa to induce hl-60 cell differentiation and adhesion. Total rna was then isolated from adherent cells, transcribed into cDna, and qrT-Pcr was ...
11
Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR
... Many recent studies have aimed to improve the methods of detection and finding new markers for CTCs. Multi- marker RT-PCR assays have become widely used for detecting both lymph node involvement and CTCs in breast ...
11
5′ Nuclease PCR Assay To DetectYersinia pestis
... Our TaqMan 59 nuclease PCR assay for detection of Y. pestis exhibited satisfactory levels of sensitivity (in the picogram range for both purified bacterial genomic DNA and cloned target sequences) and specificity ...
5
Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection
... Five serum pools were created (TB group; control group; three other microbial infection groups (BP, VZV and EV) by combining 20 samples (20 μl per sample) and mixing by inversion and 400 μl of each of these pools was ...
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