Rat cardiomyocytes
TSH inhibits SERCA2a and the PKA/PLN pathway in rat cardiomyocytes
... Caraccio et al. described how long-term increases in serum TSH concentration contribute to the development of sHT [9]; TSH alone negatively impacted cardiac function, independent of HT. Moreover, exposure to slightly ...
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Protective effects of acacetin isolated from Ziziphora clinopodioides Lam. (Xintahua) on neonatal rat cardiomyocytes
... Effects of acacetin on neonatal rat cardiomyocytes The MDA content was significantly increased from 0.14 ± 21.34 to 30.72 ± 1.40 nmol/Lafter myocardial cell injury (Table 4). Compared with Group II (30.72 ± ...
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Evidence for deactivation of both ectosolic and cytosolic 5' nucleotidase by adenosine A1 receptor activation in the rat cardiomyocytes
... cyclohexyladenosine, an adenosine A1 receptor agonist. Neither CGS21680C, and A2 receptor agonist, nor cycloheximide deactivated ectosolic and cytosolic 5'-nucleotidase. Thus, we conclude that activation of adenosine A1 ...
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Role of dopamine D2 receptors in ischemia/reperfusion induced apoptosis of cultured neonatal rat cardiomyocytes
... Primary cultures of neonatal rat cardiomyocytes were performed as previously described [8]. Newborn Wistar rats (1-3 days) were used for this study. The rats were handled in accordance with the Guide for ...
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Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia-hypercapnia
... We studied the influence of hypoxic-hypercapnic environment under the effect of hypothermia (arti - ficial hibernation) on fatty acids spectrum of inner mitochondrial membrane (iMM) lipids of rat cardiomyo - cytes ...
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The regulatory mechanisms of myogenin expression in doxorubicin-treated rat cardiomyocytes
... properties of skeletal muscle [16]. H9c2 cells cultured in lower serum conditions and/or treated with atRA (retinoic acid) differentiate from myoblasts into myotubes [17]. Here, we first induced H9C2 cell differentiation ...
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Cardioprotection Via Modulation of Calcium Homeostasis by Thiopental in Hypoxia Reoxygenated Neonatal Rat Cardiomyocytes
... pretreated with various concentrations of thiopental. Then, cardiomyocytes were subjected to a hypoxic chamber for 1 hour following reoxygenation for 5 hours. Cell viability was determined by ...
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Microcystin-LR regulates circadian clock and antioxidant gene expression in cultured rat cardiomyocytes
... Next, we induced rhythmic expression of circadian clock and antioxidant genes in H9C2 cardiomyocytes by serum shock. To determine whether MC-LR would affect the expression of the clock and antioxidant genes, a 10 ...
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IGF-1 Expression in Infarcted Myocardium and MGF E Peptide Actions in Rat Cardiomyocytes in Vitro
... in rat myocardium from 1 h up to 8 wks after myocardial infarction induced by left anterior descending coronary artery ...H9C2 rat myocardial ...in rat myocardial cells, as suggested by our in vitro ...
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Original Article Catechin protects rat cardiomyocytes from hypoxia-induced injury by regulating microRNA-92a
... Appropriate oxygen supply is critical for main- taining function of cardiomyocytes [26]. Hypoxic stimulation will disrupt the balance of the intra- cellular microenvironment, leading to irrepara- ble functional ...
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Regulation of PUMA induced by mechanical stress in rat cardiomyocytes
... Cultured cardiomyocytes thus obtained were > 95% pure as revealed by observation of contractile characteristics with a light microscope and stained with anti-desmin antibody (Dako Cytomation, Glostrup, ...
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Glucocorticoid protects rodent hearts from ischemia/reperfusion injury by activating lipocalin type prostaglandin D synthase–derived PGD2 biosynthesis
... (PD) and then subjected to glucose-free anoxia followed by reoxygenation. (A) Dead cells (red nuclei) and viable cells (green) were determined as described in Methods. Scale bars: 100 μm. (B) Viable cells were quantified ...
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Original Article Delayed cardioprotection by sevoflurane preconditioning: a novel mechanism via inhibiting Beclin 1-mediated autophagic cell death in cardiac myocytes exposed to hypoxia/reoxygenation injury
... H9c2 rat cardio- myocytes to explore the role of autophagy dur- ing H/R ...H9c2 rat cardiomyocytes with GFP-LC3 and compared the abundance of autophagic vacuoles in cells subjected to H/R to sevoflu- ...
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Specific induction of the 70 kD heat stress proteins by the tyrosine kinase inhibitor herbimycin A protects rat neonatal cardiomyocytes A new pharmacological route to stress protein expression?
... We have therefore attempted to ascertain the ability of herbimycin-A to induce hsp’s in primary cultured neonatal rat cardiomyocytes, using genistein, another tyrosine kinase inhib- itor, for comparison. ...
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Original Article miR-124 regulates myocardial ischemia reperfusion injury
... The rat model of myocardial ischemia-reperfusion was estab- ...the rat cardiomyocytes were isolated, extracted and cultured; then miR-124 was transfected into the cells, the test group cells were ...
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Original Article Zinc sulphate preconditioning alleviates rat myocardial hypoxia reoxygenation injury in vitro: activation of the Nrf2/ARE signaling pathway
... Sprague-Dawley rat cardiomyocytes into the following four groups: Con group ...of cardiomyocytes, but luteolin canceled such protective ...alleviate rat myocardial hypoxia reoxygenation injury ...
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MiR-499-5p protects cardiomyocytes against ischaemic injury via anti-apoptosis by targeting PDCD4
... neonatal rat cardiomyocytes induced by ...cultured cardiomyocytes in ...the rat AMI models with agomir reduced the myocardial infarct size through decreasing the cardiomyocytes ...
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Microarray analysis of NSAIDs-treated cardiomyocytes to search for genes involved in COX-2 inhibitor cardiotoxicity
... We found that celecoxib and diclofenac induced wide-scale changes in the gene expression of neonatal rat cardiomyocytes. Pathway analysis of the microarray data showed changes in critical cell processes, ...
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Disappearance of cyclin A correlates with permanent withdrawal of cardiomyocytes from the cell cycle in human and rat hearts
... Because cardiomyocytes in adult mammals withdraw permanently from the cell cycle and thus cannot regenerate after injury, we examined cyclin expression during development by comparing cyclin A-E mRNA levels in ...
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The cardioprotective effect of danshen and gegen decoction on rat hearts and cardiomyocytes with post ischemia reperfusion injury
... Calcium ion stunning also plays a critical role in the initiation of apoptosis in cardiomyocyte in an I/R injury [50]. Our present results revealed that DG treatment greatly inhibited a H/R-induced elevation of cytosolic ...
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