rat islets
The Roles of Intracellular and Extracellular Ca++ in Glucose Stimulated Biphasic Insulin Release by Rat Islets
... into islets of Langerhans, has been used to study the roles of intra- and extracellular calcium in the two phases of glucose- induced insulin ...in rat islets after maintenance in tissue culture for ...
9
Heterogeneity and compartmental properties of insulin storage and secretion in rat islets
... isolated rat islets were used for pulse-labeling experiments, after which proinsulin and insulin were purified ...in islets; it was secreted at a higher fractional rate than immunoreactive insulin, ...
11
Regulatory effects of glucose on the catalytic activity and cellular content of glucokinase in the pancreatic beta cell Study using cultured rat islets
... Glucose regulates the cellular content of glucokinase in the pancreatic beta cell by altering the level of the enzyme. We investigated the existence of a second regulatory pathway, an alteration in the catalytic ...
6
Long-term graft function of cryostored alginate encapsulated rat islets
... cd rat islets 2, 12 and 56 weeks after ...encapsulated rat islets can reverse diabetes for a long period of time similarly to non-cryopreserved encapsulated rat islets without ...
5
Role of insulin secretagogues in the regulation of somatostatin binding by isolated rat islets
... in islets in the presence of various substances known to promote secretion vesicle migration and fusion with the plasma membrane and insulin ...release. Rat islets were incubated with glucose, 30 and ...
6
Dependency of cyclic AMP induced insulin release on intra and extracellular calcium in rat islets of Langerhans
... monophasic release. Decreasing extracellular Ca++ to 0.1 mM during stimulation reduced the glucose effect by 80% but did not alter IBMX-induced release. Both glucose and IBMX stimulated 45Ca++ uptake (5 min). 45Ca++ ...
10
Evidence for presence of insulin receptors in rat islets of Langerhans
... saturable with respect to time and insulin concentration and depended on the number of incubated islets. Furthermore, bound insulin was displaced by native insulin in a dose- dependent manner. Bound [125I]insulin ...
9
Synthesis and release of proinsulin and insulin by isolated rat islets of Langerhans
... Release of newly synthesized proinsulin and insulin occurred under the following conditions: a incubation for greater than 60 min; b glucose concentrations above 5.3 mmoles/liter; c incu[r] ...
12
Functional differences between rat islets of ventral and dorsal pancreatic origin
... in islets from the ventral (ventral islets) and dorsal (dorsal islets) areas of the rat ...Dorsal islets contained 10 times as much glucagon as ventral islets, whereas insulin ...
10
Glucose Stimulated 45Calcium Efflux from Isolated Rat Pancreatic Islets
... Kinetics of 45 Ca efflux and insulin release were studied in collagenase-isolated rat islets during 2-h perifusions with calcium-depleted (0.05 mM) bicarbonate-phosphate buffer containing 2.2 mM glucose. ...
8
Optimization of pancreatic islet isolation from rat and evaluation of islet protective potential of a saponin isolated from fruits of Momordica dioica
... pathway. Rat insulinoma RINm5F cells and isolated rat islets were treated with IL-1β and IFN-γ to induce cytotoxicity reported (Song et ...the islets obtained after handpicking were round with ...
11
Adenoviral-Induced Islet Cell Cytotoxicity Is Not Counteracted by Bcl-2 Overexpression
... from rat islets were infected with different con- centrations of the viruses (1, 10, 100, and 1000 ...Dispersed rat islet cells were used be- cause we observed low transduction efficiency when ...
9
Leptin rapidly suppresses insulin release from insulinoma cells, rat and human islets and, in vivo, in mice
... Obesity is associated with diabetes, and leptin is known to be elevated in obesity. To investigate whether leptin has a direct effect on insulin secretion, isolated rat and human is- lets and cultured insulinoma ...
9
Transduction of rat pancreatic islets with pseudotyped adeno associated virus vectors
... human islets have also been ...and rat to mouse trans- fer of islets provides a model ...of rat islets, versus murine islets, also makes the use of rat islets ...of ...
10
Glucagon release from rat pancreatic islets A combined morphological and functional approach
... The release of glucagon induced in isolated rat islets by arginine or by calcium deprivation has been subjected to combined functional and morphological quantifications. Arginine- stimulated glucagon ...
10
Biosynthesis and release of thyrotropin releasing hormone immunoreactivity in rat pancreatic islets in organ culture Effects of age, glucose, and streptozotocin
... from islets of both 5-d-old (newborn) and 30-d-old (adult) rats with a maximum on the second day of culture ...isolated islets from newborn rats (22.4 +/- 2.3 fmol/islet) than in adult rat ...
8
Immunohistochemical and morphometric study of the effect of maternal diabetes on rat foetal pancreatic islets
... The effect of maternal diabetes on the foetal endocrine pancreas has been the subject of extensive studies, but a detailed quantitative immunohistochemical investigation is not available. This study was therefore ...
6
Characterization of mixed syngeneic allogeneic and syngeneic xenogeneic islet graft rejections in mice Evidence of functional impairment of the remaining syngeneic islets in xenograft rejections
... mouse islets or xenogeneic rat islets, or fetal porcine islets were implanted under the renal capsule of C57BL/6 mice either alone or carefully mixed with syngeneic ...syngeneic islets, ...
8
Rapid and reversible secretion changes during uncoupling of rat insulin producing cells
... of rat pancreatic islets of Langerhans, primary dispersed islet cells, and cells of the RINm5F line, during short-term exposure to ...intact islets and abolished their normal secretory response to ...
11
STUDY OF ORAL HYPOGLYCEMIC ACTIVITY OF RHIZOMES OF HEMIDESMUS INDICUS R Br IN ALBINO WISTAR RATS
... ABSTRACT: The present study was carried out to evaluate oral hypoglycemic activity of rhizome of Hemidesmus indicus R.Br. in Albino wistar rats. Hemidesmus indicus (Family: Asclepiadaceae) is a commonly known Indian ...
6