rat liver microsomes
Liquid chromatography tandem mass spectrometry method for the quantification of vandetanib in human plasma and rat liver microsomes matrices: metabolic stability investigation
... Vandetanib (VNT) is a new oral tyrosine kinase inhibitor that acts mainly by inhibiting vascular endothelial growth factor receptor (VEGFR). Fast, specific, sensitive and validated LC–MS/MS was established for the ...
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Effects of Eleutheroside B and Eleutheroside E on activity of cytochrome P450 in rat liver microsomes
... Background: Chemicals of herbal products may cause unexpected toxicity or adverse effect by the potential for alteration of the activity of CYP450 when co-administered with other drugs. Eleutherococcus senticosus (ES), ...
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Effect of water miscible organic solvents on p-nitrophenol hydroxylase (CYP2E1) activity in rat liver microsomes
... solvent free incubation (control) to evaluate the effect of solvents was missing while in the latter case complete re-solubilization of substrate was assumed. In the present study, we chose to use a substrate ...
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Effect of water-miscible organic solvents on CYP450-mediated metoprolol and imipramine metabolism in rat liver microsomes
... solvent control was missing while in latter approach complete resolubilization of substrate was assumed. In the present study, we have studied the effect of ten water miscible organic solvents methanol (MeOH), ethanol ...
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Triptolide Induces hepatotoxicity via inhibition of CYP450s in Rat liver microsomes
... However, the underlying mechanisms of TP-induced toxicity mediated by the CYP450 family have not been well delineated. Since abnormal drug metabolism by CYP450s also plays an important role in the hepatotox- icity, the ...
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Corticosteroids increase superoxide anion production by rat liver microsomes
... Superoxide anion production by liver microsomes from intact, adrenalectomized, and cortisoltreated adrenalectomized rats has been determined. The amount formed was roughly proportionate to the amount of ...
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In vitro Metabolism of Risperidone on Isolated Microsomes of Rat Liver: Metabolite Identification and Profiling by RP-HPLC and LC-MS Techniques
... isolated microsomes of rat ...isolated microsomes derived from rat ...with rat liver microsomes resulted in the formation of 2 major hydroxy ...
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The synergistic effect of daidzein and α tocopherol or ascorbic acid on microsome and LDL oxidation
... in rat liver microsomes. Liver microsomes of rats were iso- lated as described previously (Cai et ...1951). Microsomes were suspended in ...(v/v). Microsomes were ...
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LC–MS/MS method for the quantification of masitinib in RLMs matrix and rat urine: application to metabolic stability and excretion rate
... Masitinib (MST) is a selective tyrosine kinase inhibitor. Validated liquid chromatography tandem mass spectrometric method (LC–MS/MS) was developed for the quantification of MST in rat liver ...
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In vitro and in vivo evaluation of CYP1a interaction potential of terminalia arjuna bark
... in rat and human liver ...in rat liver microsomes while ...human liver microsomes, respectively for ...both rat liver microsomes and human ...
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Construction of a novel quinoxaline as a new class of Nrf2 activator
... human liver microsomes; RLM: rat liver microsomes; MLM: mouse liver microsomes; DMEM: Dublecco’s modified eagle medium; FBS: fetal bovine serum; MTT: ...
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Inhibitory effect of celecoxib on agomelatine metabolism in vitro and in vivo
... The incubation mixture contained HLMs or rat liver microsomes, 1 M potassium phosphate buffer (pH 7.4), agomelatine (100 µΜ ; approximately the K m of human and rat liver ...
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Relationship between antioxidant and anxiolytic activity of standardized extracts of Melissa officinalis and Rosmarinus officinalis
... of rat liver microsomes, and prevented the oxidation of microsomal thiols indicating the presence of antioxidant compounds in the extracts (Figure ...
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Characterization of major metabolites of polymethoxylated flavonoids in Pericarpium Citri Reticulatae using liver microsomes immobilized on magnetic nanoparticles coupled with UPLC/MS–MS
... The peels of citrus fruits (Pericarpium Citri Reticulatae, PCR) have long been used in traditional Chinese medicines (TCMs). Polymethoxylated flavonoids (PMFs) were found to be the main components present in PCR ...
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Nitrosamines and oesophageal cancer. A study of factors influencing the distribution and metabolism of nitrosamines and the enzymes responsible for their activation
... f rat liver microsomes (Raunio, 1988), so the coumarin metabolism is carried out by a P450 which is not closely related to mouse ...2A5. Rat intestine and lung express P450 2A3, but this is ...
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Original Article Human UDP-glucuronosyltransferase 1A1, 1A7, 1A8, 1A9 and 1A10 are mainly responsible for icariside II-7- O-glucuronidation
... mice liver micro- somes (MLM) followed the Hill equation (Figure 5B), while the icariside II-7-O-glucuronidation by rat liver microsomes (RLM) (Figure 5A), monkey liver ...
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Competitive inhibition of bile acid synthesis by endogenous cholestanol and sitosterol in sitosterolemia with xanthomatosis Effect on cholesterol 7 alpha hydroxylase
... in rat and human hepatic microsomes. In untreated rat liver microsomes, the 7 alpha-hydroxylation of cholesterol was higher than that of cholestanol ...the microsomes by acetone ...
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Characteristics of intracellular Ca<sup>2+</sup> signals consisting of two successive peaks in hepatocytes during liver regeneration after 70% partial hepatectomy in rats
... by day 3 after 70% PH, sharply increased to the peak at 6 hours before day 4, and then returned to the control level at 3 hours within day 4. Thus, the expression profile of the VEGF gene suggests that induction of ...
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Glucuronidation of 6 alpha hydroxy bile acids by human liver microsomes
... The glucuronidation of 6-hydroxylated bile acids by human liver microsomes has been studied in vitro; for comparison, several major bile acids lacking a 6-hydroxyl group were also investigated. ...
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The Effect of Inducers and Inhibitors of Monooxygenase on the Activity Nitrergic System in the Microsomes in the Ischemic Liver
... the liver, and it plays the key role in this issue ...in microsomes isolated from hepatocytes in the development of the pathological liver ...
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