Rat pancreatic islets
Biosynthesis and release of thyrotropin releasing hormone immunoreactivity in rat pancreatic islets in organ culture Effects of age, glucose, and streptozotocin
... isolated islets and in medium from rat pancreatic islets maintained in organ ...both islets and culture medium was eluted in the same position as synthetic TRH by ion-exchange and gel ...
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Adenosine Triphosphatases of Rat Pancreatic Islets: COMPARISON WITH THOSE OF RAT KIDNEY
... of rat pancreatic ...endocrine pancreatic function upon ATPases in homogenates of islets and kidney, the latter being a tissue which would not be expected to have a stimulus-secretion response ...
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Somatostatin and Epinephrine Induced Modifications of 45Ca++ Fluxes and Insulin Release in Rat Pancreatic Islets Maintained in Tissue Culture
... by rat pancreatic islets after 2 days in tissue culture and with regard to 45 Ca ++ efflux from islets loaded with the radio-isotope during the 2 days of ...
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Possible role for calmodulin in insulin release Studies with trifluoperazine in rat pancreatic islets
... The role of calmodulin in insulin secretion from rat pancreatic islets has been examined by the use of trifluoperazine, an inhibitor of calmodulin-Ca++-directed functions. It was found that 30 microM ...
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Phosphoinositide metabolism and insulin secretion from isolated rat pancreatic islets
... isolated rat pancreatic islet is accompanied by detectable alterations in the phosphatidylinositol metabolism of this ...that rat pancreatic islets incubated with tritiated myo-inositol ...
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Glucagon release from rat pancreatic islets A combined morphological and functional approach
... The release of glucagon induced in isolated rat islets by arginine or by calcium deprivation has been subjected to combined functional and morphological quantifications. Arginine- stimulated glucagon ...
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Transduction of rat pancreatic islets with pseudotyped adeno associated virus vectors
... remaining islets and the wash was also filtered through the wire ...The islets were washed several times with DMEM and resuspended in 10 ml of ...DMEM. Islets were hand picked for later ...
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Effects of a novel curcumin derivative on insulin synthesis and secretion in streptozotocin-treated rat pancreatic islets in vitro
... In this study, NCD increased the zinc levels in pancre- atic islets whether the treatment was performed before or after STZ exposure. Elevation of zinc levels augments insulin synthesis and release. Malhotra et ...
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Evidence for the Involvement of Na/Ca Exchange in Glucose induced Insulin Release from Rat Pancreatic Islets
... Since ouabain increases Nai+ 25, 26, the slow increase of 45Ca++ efflux is probably due to a continued gradual increase of cytosol Ca++ in turn due to the combined effects of inhibited N[r] ...
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Glucose Stimulated 45Calcium Efflux from Isolated Rat Pancreatic Islets
... unstimulated islets suggests that there are at least two, and probably three, different intracellular Ca compartments (not including the extracellular sucrose ...
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Role of Ca2+ in secretagogue stimulated breakdown of phosphatidylinositol in rat pancreatic islets
... an inhibitor of phosphodiesterase, which elevates [3H]PI, as a percentage of the 3H remaining in the cAMP levels, had no effect on 3H efflux despite stim- islet, was reduced significantl[r] ...
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Rapid and reversible secretion changes during uncoupling of rat insulin producing cells
... of rat pancreatic islets of Langerhans, primary dispersed islet cells, and cells of the RINm5F line, during short-term exposure to ...intact islets and abolished their normal secretory ...
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A Study of the Effects of Electromagnetic Field on Islets of Langerhans and Insulin Release in Rats
... of rat pancreatic islets accompanied with insulin release changes, the evidence for effects of EMF on insulin release and structure of islets remains controversial issue ...of ...
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Optimization of pancreatic islet isolation from rat and evaluation of islet protective potential of a saponin isolated from fruits of Momordica dioica
... of rat pancreatic islets, islet viability, potential, insulin secretion, and intra-islet contents was performed, and also, the insulin assay protective properties were ...the islets secreted ...
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The unsuccessful hunt for pancreatic cancer biomarkers – time to Search deeper in the proteome
... of pancreatic cancers with implications in cell growth, survival, apoptosis, or response to extracellular ...in pancreatic cancer as a viable option for biomarker searches in various stages of the disease ...
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Adaptations of alpha2 and beta cells of rat and mouse pancreatic islets to starvation, to refeeding after starvation, and to obesity
... To expand our knowledge on a2-cell function in diabetes-like states, we now studied the effect of "starvation-diabetes" and of diabetes in obesity on a2cell responsiveness to stimulation[r] ...
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Age dependent changes in the exocytotic efficacy in Kir6 2 ablated mouse pancreatic β cells
... Pancreatic slices were prepared as previously described [23]. Briefly, 2% gelling agarose (Seaplaque GTG agarose, BMA, Walkersville, Md., USA) melted in warm (37˚C) extracellular solution (ECS) was injected into ...
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T cells expressing chimeric antigen receptor promote immune tolerance
... This permitted efficient modular use of previously characterized mAbs conjugated with FITC that (a) bound mAbCAR, (b) activated T cell function in vitro and in vivo, and (c) promoted homing of T cells expressing mAbCAR ...
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A Metabolomic Approach (1H HRMAS NMR Spectroscopy) Supported by Histology to Study Early Post-transplantation Responses in Islet-transplanted Livers
... 34). The first experimental group was composed of 7 non-diabetic rats (ND). Diabetes was induced in 75 rats by intraperitoneal injection of streptozotocin (Sigma-Aldrich, St. Louis, MO) at 75 mg/kg freshly dissolved in ...
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Pancreatic islet transplantation
... of pancreatic islets is comprised of several ...the islets are maintained in the preservation solution until the begin- ning of the isolation ...the pancreatic duct and the pan- creas is cut ...
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