RAT VENTRICULAR MYOCYTES
Ionic Mechanisms of Desflurane on Prolongation of Action Potential Duration in Rat Ventricular Myocytes
... in rat ventricular myocytes. Materials and Methods: Rat ventricular myocytes were enzymatically isolated and studied at room tempera- ...
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Hypoxia alters the subcellular distribution of protein kinase C isoforms in neonatal rat ventricular myocytes
... Cardiac myocytes coexpress multiple protein kinase C (PKC) isoforms which likely play distinct roles in signaling pathways leading to changes in contractility, hypertrophy, and ischemic ...neonatal rat ...
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Carbon Nanohorns Promote Maturation of Neonatal Rat Ventricular Myocytes and Inhibit Proliferation of Cardiac Fibroblasts: a Promising Scaffold for Cardiac Tissue Engineering
... Cardiac tissue engineering (CTE) has developed rapidly, but a great challenge remains in finding practical scaffold materials for the construction of engineered cardiac tissues. Carbon nanohorns (CNHs) may be a potential ...
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Cell cell signaling between adult rat ventricular myocytes and cardiac microvascular endothelial cells in heterotypic primary culture
... within ventricular myocardium, known to be important during cardiac development, remains physiologically relevant in the adult ...cardiac myocytes, are unknown, as their cells of origin within ...
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Rate-dependent Ca2+ signalling underlying the force-frequency response in rat ventricular myocytes: a coupled electromechanical modeling study
... cAMP may directly or indirectly activate various intracellular targets including ion chan- nels and exchangers. The indirect modulation involves activation of cAMP-dependent Protein kinase A (PKA) before modulation of ...
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The effects of tumor necrosis factor alpha on systolic and diastolic function in rat ventricular myocytes
... Impaired cardiac function is a well-recognized feature of sepsis and septic shock (Kumar et al. 2001a; Court et al. 2002). Manifestations of this dysfunction fall into two broad categories. (1) Systolic dysfunction ...
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Changes of SERCA activity have only modest effects on sarcoplasmic reticulum Ca2+ content in rat ventricular myocytes
... All procedures accord to the UK Animals (Scientific Procedures) Act 1986 and University of Manchester Ethical Review Process. Rats (male Wistar, 200–250 g) were killed by stunning and cervical dislocation and ...
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Endothelin enhances the contractile responsiveness of adult rat ventricular myocytes to calcium by a pertussis toxin sensitive pathway
... adult rat ventricular cells, endothelin's action was slow in onset but very long lasting with an EC50 of 50 pM that approximates the reported KD of the peptide for its receptor in rat ...
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Glycolytic inhibition: effects on diastolic relaxation and intracellular calcium handling in hypertrophied rat ventricular myocytes
... We tested the hypothesis that glycolytic inhibition by 2-deoxyglucose causes greater impairment of diastolic relaxation and intracellular calcium handling in well-oxygenated hypertrophied adult rat myocytes ...
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Hypertrophic stimuli induce transforming growth factor beta 1 expression in rat ventricular myocytes
... adult rat ventricular myocardium freshly dissociated into myocyte and nonmyocyte cellular fractions, the preponderance of TGF-beta 1 mRNA visualized by Northern hybridization was in the nonmyocyte ...caused ...
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<em>N</em>-n-butyl haloperidol iodide inhibits H<sub>2</sub>O<sub>2</sub>- induced Na<sup>+</sup>/Ca<sup>2+</sup>-exchanger activation via the Na<sup>+</sup>/H<sup>+</sup> exchanger in rat ventricular myocytes
... Our results show that acute exposure of cardiac myocytes to 100 µ M H 2 O 2 causes the I NCX to increase, along with a rapid activation of MEK and an increase in NHE activity. The H 2 O 2 - induced I NCX increase ...
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Modeling CICR in rat ventricular myocytes: voltage clamp studies
... each ventricular cell contains 10,000 of these dyadic Ca 2+ release units, and that they are associated with the fraction of the SL membrane that is coupled with the ...
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Systolic [Ca2+ ]i regulates diastolic levels in rat ventricular myocytes
... Care and use of animals were in accordance with The UK Animals (Scientific Procedures) Act, 1986 and Directive 2010/63/EU of the European Parliament. The experiments were approved by the University of Manchester Ethical ...
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Systolic [Ca2+ ]i regulates diastolic levels in rat ventricular myocytes
... Animals were cared for and used in accordance with The UK Animals (Scientific Procedures) Act, 1986 and Directive 2010/63/EU of the European Parliament. The experiments were approved by the University of Manchester ...
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Direct contact between sympathetic neurons and rat cardiac myocytes in vitro increases expression of functional calcium channels
... neonatal rat ventricular myocytes with and without sympathetic ...of myocytes were assessed by an optical-video ...innervated myocytes compared with control cells at ...and ...
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Highly efficient gene transfer into adult ventricular myocytes by recombinant adenovirus
... Molecular dissection of mechanisms that govern the differentiated cardiac phenotype has, for cogent technical reasons, largely been undertaken to date in neonatal ventricular myocytes. To circumvent ...
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Mechanical strain activates BNP gene transcription through a p38/NF κB–dependent mechanism
... We have documented previously that mechanical strain is a potent activator of hBNP gene transcription in neonatal rat ventricular myocytes (4, 39). The present work extends those findings in ...
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WIPI1 is a conserved mediator of right ventricular failure
... cardiac myocytes under conditions mimicking the neu- rohormonal activation of ...Neonatal rat ventricular myocytes (NRVMs) were transfected with scramble or Wipi1-specific siRNAs and then ...
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CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes
... Although readily detectable in the heart, nothing has been published about the function of CIP4 and other F-Bar proteins in the cardiac myocyte. In other cell types, CIP4 has been shown to regulate epidermal growth ...
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Significance of Na+ current in the excitability of atrial and ventricular myocardium of the fish heart
... recycled using a peristaltic pump. After enzymatic digestion, atrium and ventricle were excised, placed in fresh low-Na + solution in a Petri dish and cut into small pieces with scissors. Single cells were released by ...
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