RAW 264.7
5 Amino Salicylic Acid and Sodium Salicylate Inhibit Activation of IκB Kinase and P38 γ MAP Kinase in LPS Induced RAW 264 7 cell
... potential to regulate the levels of the aforementioned cytokines in LPS stimulated RAW 264.7 cells. LPS induced cells have significantly increased the levels of cytokines compared to the vehicle control (15.86 ± ...
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Elucidation of anti inflammatory constituents in Hypericum perforatum extracts and delineation of mechanisms of anti inflammatory activity in RAW 264 7 mouse macrophages
... bone marrow-derived macrophages treated with LPS for 0-21 hours. LPS-inducible genes had obvious functions such as endocytosis and phagocytosis, along with 18% of the differentially expressed genes involved in cell ...
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Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF kappaB signaling pathway in lipopolysaccharide stimulated RAW 264 7 macrophages
... plemented with 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin in the presence or absence of EEPC. The cell viability was measured using a MTT assay. Briefly, RAW 264.7 cells (5 × 10 5 cells/ml) were ...
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STANDARDIZED SUPERCRITICAL CO2 EXTRACT OF ACANTHUS ILICIFOLIUS (LINN ) LEAVES INHIBITS THE PRO INFLAMMATORY CYTOKINE TUMOR NECROSIS FACTOR Α IN LIPOPOLYSACCHARIDE ACTIVATED MURINE RAW 264 7 MACROPHAGE CELLS
... viability assays. Different incubation period did not have any effect on cell viability/cytotoxicity, so 24 h of incubation was selected for TNF-α assay. RAW 264.7 macrophages were cultured in 24-well plates with ...
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Inhibition of lipopolysaccharide induced nitric oxide and prostaglandin E2 production by chloroform fraction of Cudrania tricuspidata in RAW 264 7 macrophages
... Figure 2 Effect of CTM on LPS-induced NO levels in RAW 264.7 cells. (A) Cells were treated with different concentrations of CTM and CTC (62, 125, 250, 500 μ g/ml) for 1 h, then with LPS (10 μ g/ml) for 24 h. ...
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Anti Inflammatory Effect of 3 Methylcarbazoles on RAW 264 7 Cells Stimulated with LPS, Polyinosinic Polycytidylic Acid and Pam3CSK
... RAW 264.7 murine macrophage cell line was obtained from the Korea Cell Line Bank (Seoul, Korea). These cells were grown at 37˚C in DMEM medium supplement with 10% FBS, penicillin (100 units/ml), and streptomy- cin ...
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Inhibition of lipopolysaccharide (LPS) induced neuroinflammatory response by polysaccharide fractions of Khaya grandifoliola (C D C ) stem bark, Cryptolepis sanguinolenta (Lindl ) Schltr and Cymbopogon citratus Stapf leaves in raw 264 7 macrophages and U87 glioblastoma cells
... oxide synthase (iNOS). Cytokines, such as interleukin (IL)- 1, IL-6, and tumor necrosis factor (TNF), have been known to play important roles in pro-inflammatory response [32, 33]; and iNOS is up-regulated by secretion ...
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An ethyl acetate fraction derived from Houttuynia cordata extract inhibits the production of inflammatory markers by suppressing NF кB and MAPK activation in lipopolysaccharide stimulated RAW 264 7 macrophages
... Activation of NF-κB, an essential transcription factor in the inflammatory response, occurs following the phos- phorylation, ubiquitination, and proteolytic degradation of IκBα. Thus, p65 levels in cytoplasmic and ...
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Pleurotus giganteus (Berk Karun & Hyde), the giant oyster mushroom inhibits NO production in LPS/H2O2 stimulated RAW 264 7 cells via STAT 3 and COX 2 pathways
... maximum dose (100 μg/ml) tested, showed a significant (p < 0.05) decrease in cell viability. The cell viability for this particular concentration was only 39.9% as compared to the control. This could be due to the ...
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Water extract of Cnidii Rhizoma suppresses RANKL induced osteoclastogenesis in RAW 264 7 cell by inhibiting NFATc1/c Fos signaling and prevents ovariectomized bone loss in SD rat
... For differentiation, RAW 264.7 cells were treated with RANKL (100 ng/ml) and CR for 5 days. For the TRAP staining, mature osteoclasts were washed with DPBS (Gibco, Gaithersburg, MD, USA), the differentiated ...
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Sargassum horneri (Turner) C Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO 1 signaling in RAW 264 7 cells
... Environmental studies, Ibaraki, Japan. 3-(4,5-dimethylthia- zol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All analytical grade organic solvents were purchased from ...
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Mesuaferrin A: Bio Active Flavonoid Inhibits LPS Stimulated Inflammatory Response via Down Regulation of NF κB and MAPK Signaling Pathways in RAW 264 7 Cell Lines
... Inhibitory effects of Mesuaferrin - A on NF-κB P 65 nuclear translocation in LPS stimulated RAW 264.7 cells. These results indicated that the anti-inflammatory effect of Mesuaferrin – A is mediated, through ...
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Differences in anti inflammatory effect of immature and mature of Rubus coreanus fruits on LPS induced RAW 264 7 macrophages via NF κB signal pathways
... present study, IRF contained a higher quantity of EA than MRF did, indicating that the EA component in IRF inhib- ited the production of pro-inflammatory cytokines and inflammation-related factors in LPS-stimulated ...
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Gyeji tang water extract exerts anti inflammatory activity through inhibition of ERK and NF κB pathways in lipopolysaccharide stimulated RAW 264 7 cells
... To examine whether GJT can influence secretion of pro- inflammatory cytokines TNF-α and IL-6, GJT extracts were added into LPS-stimulated RAW 264.7 cells. LPS significantly enhanced the amounts of secreted TNF-α ...
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Herbal formula SC E1 suppresses lipopolysaccharide stimulated inflammatory responses through activation of Nrf2/HO 1 signaling pathway in RAW 264 7 macrophages
... Because Nrf2 translocation is correlated with the expression of anti-oxidant proteins protecting against inflammation-induced oxidative damage, this study ex- amined whether SC-E1 regulates HO-1, a target gene of Nrf2, ...
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Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264 7 cells
... Intracellular reactive oxygen species scavenging activity RAW 264.7 cells were plated at 1 × 10 6 cells/well. After 4 h, the cells were treated with 10, 50, and 100 μg/mL C. officianalis and LPS for 24 h. After ...
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Molecular basis of the anti inflammatory potential of a diarylheptanoid in murine macrophage RAW 264 7 cells
... RAW 264.7 cells were incubated with or without LPS (1 μg/mL) and with the optimized dose of AOHE and DAH incubated for 24 h. Cells were homogenized using TRI- zol® reagent and RNA was isolated by phenol-chloro- ...
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Synergistic interaction between C5a and NOD2 signaling in the regulation of chemokine expression in RAW 264 7 macrophages
... well studied. Complement signaling via C5aR can both activate and inhibit TLR4 signaling leading to the ex- pression of inflammatory cytokines; this response is de- pendent on the cellular/physiological context ...
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Citrus unshiu flower inhibits LPS-induced iNOS and COX-2 via MAPKs in RAW 264.7 macrophage cells
... In this study, CUF-EA inhibited the phosphorylation of ERK1/2, JNK1/2, and p38 in LPS- stimulated RAW 264.7 cells. In conclusion, the results of the present study provide the first evidence that CUF-EA inhibits ...
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Differential gene expression mediated by 15 hydroxyeicosatetraenoic acid in LPS stimulated RAW 264 7 cells
... LPS-stimulated macrophage-like RAW 264.7 cells were treated for 24 h with 40 μM 15(S)-HETE based on the esti- mate that trophozoites and Hz contained 33–39 μmol 15- HETE/L RBC [5]. Statistically significant (p ≤ ...
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