Real-time PCR test
Multiplexed Real Time PCR Assay for Discrimination of Plasmodium Species with Improved Sensitivity for Mixed Infections
... of real-time PCR for the diagnosis of malaria has been hampered by poor sensitivity for the detection of mixed ...nested PCR as the “gold standard.” This test has been implemented for ...
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Evaluation of a Commercial Real Time PCR Kit for Detection of Dengue Virus in Samples Collected during an Outbreak in Goiânia, Central Brazil, in 2005
... diagnostic test with high sensitivity that is applicable during the early symptomatic ...to test two distinct molecular methods on samples from suspected dengue cases during an outbreak in Central ...by ...
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Diagnostic performance of the loop mediated isothermal amplification (LAMP) based illumigene® malaria assay in a non endemic region
... (RDT, microscopy and PCR) (Table 1). No invalid LAMP results were observed. In a first set of 20 Plasmodium positive samples stored at − 80 °C for up to 1 year, all samples showed a positive result with the ...
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Performance of a methylation specific real-time PCR assay as a triage test for HPV-positive women
... algorithm. PCR is performed using oncgnostics custom-made real-time PCR mastermix containing a hot- start DNA ...plex real-time PCR using EvaGreen (Biotium, USA) as ...
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Correction: Key differences between 13 KRAS mutation detection technologies and their relevance for clinical practice
... real-time PCR assays (therascreen KRAS RGQ PCR Kit, Qiagen; cobas KRAS Mutation Test, Roche Diagnostics; Idylla, Biocartis, Mechelen, Belgium); matrix-assisted laser ...
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Real Time PCR Assay for Rapid and Accurate Detection of Point Mutations Conferring Resistance to Clarithromycin in Helicobacter pylori
... by real-time PCR. A real-time PCR-based PCR-hybridization assay was used directly on DNA obtained from gastric biopsies to detect point mutations conferring resis- tance ...
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Specific Detection of Rinderpest Virus by Real Time Reverse Transcription PCR in Preclinical and Clinical Samples from Experimentally Infected Cattle
... tic test capability for differentiation between RPV and PPRV would be very useful in the surveillance of suspected cases after ...detection test in place will be critical for disease ...
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Key differences between 13 KRAS mutation detection technologies and their relevance for clinical practice
... real-time PCR assays (therascreen KRAS RGQ PCR Kit, Qiagen; cobas KRAS Mutation Test, Roche Diagnostics; Idylla, Biocartis, Mechelen, Belgium); matrix-assisted laser ...
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Real Time PCR Method for Detection of Zygomycetes
... of real-time PCR in the diagnosis of zygomycete infection is the reduction of turn- around time for results from days to hours, a difference that can have a significant positive impact on ...
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Clinical Validation of a Point of Care Multiplexed In Vitro Immunoassay Using Monoclonal Antibodies (the MSD Influenza Test) in Four Hospitals in Vietnam
... the time to clinical decision making. An investiga- tional POC test based on a multiplexed immunoassay was developed by Meso Scale Diagnostics, LLC (MSD), with the objective to make a more sensitive rapid ...
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... The test strains originated from 17 different species, which underlines the ubiquitous nature and wide host range of M. avium subsp. avium. To our knowledge, isolation of M. avium subsp. silvaticum from wild ...
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Comparison of real time PCR and microscopy for malaria parasite detection in Malawian pregnant women
... pfldh PCR assay - was not associated with poor birth outcomes compared with aparasitaemic ...antigen test or PCR assays [20,21]. In contrast, real-time PCR positivity was ...
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Comparison of Leishmania OligoC TesT PCR with Conventional and Real Time PCR for Diagnosis of Canine Leishmania Infection
... kDNA PCR, despite having similar overall sensitivities, showed only moderate agreement, indicating a degree of ...the PCR-hybridization method could have resulted from a lower DNA extraction efficiency ...
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Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA
... seminested real-time PCR and, in parallel, the corresponding single-step real-time PCR ...in real-time PCR, are shown in Table ...seminested ...
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Real time pcr for genotyping of hepatitis c virus: evaluation and application
... qualitative PCR, except six serum samples showing negative HCV RNA test and were excluded from ...RT PCR and real time ...with real time PCR technique and only ...
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Real Time PCR Assay for Diagnosis of Entamoeba histolytica Infection
... of real-time-PCR, antigen detection, and tra- ditional-PCR assays on stool and liver abscess pus ...detection test, traditional nested PCR, and real-time PCR ...
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Multiplex Real Time PCR shortTUB Assay for Detection of the Mycobacterium tuberculosis Complex in Smear Negative Clinical Samples with Low Mycobacterial Loads
... Finally, eight of the positive culture samples tested negative for both targets in the assay. In two of these cases, only the solid culture (Coletsos medium; bioMérieux Clinical Diagnostics, Spain) was positive, after ...
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Exon duplications in the ATP7A gene: Frequency and Transcriptional Behaviour
... for PCR amplification with ATP7A-specific primer pairs flanking the duplica- tion of ...2 PCR Kit (Clon- tech) was used for the ...the PCR amplification pri- mers. For two patients, we performed ...
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External Quality Assessment for Enterovirus 71 and Coxsackievirus A16 Detection by Reverse Transcription PCR Using Armored RNA as a Virus Surrogate
... VLPs), was distributed to 54 laboratories that perform molecular diagnosis of hand, foot, and mouth disease (HFMD) virus infections. A total of 41 data sets from 41 participants were returned; 5 (12.2%) were generated ...
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Activation of Toll-Like Receptors 2 by High-Mobility Group Box 1 in Monocytes from Patients with Ischemic Stroke
... thromboplastin time upper limit of normal, current use of anticoagulant with protrombin time >15 seconds and blood glucose concentration <50 mg/dL ...
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