Real-time RT-PCR assay
Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... duplex real-time RT- PCR assay was developed to identify H5N6 subtype ...duplexed assay are as follows: low cost, low risk of carryover contamination, and re- duced hands-on ...
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A one step real time RT PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)
... biological assay (virus inoculation and symptom expression in diagnostic plant species), ELISA (enzyme- linked immunosorbent assay) [15], immunoelectron mi- croscopy, RT-PCR (reverse ...
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REAL TIME RT PCR ASSAY OF GENE FUMONISIN FUM5 RESPONSIBLE FOR MYCOTOXIN PRODUCTION ON RICE
... detection assay was reported by Karthikeyan et ...the Real-Time RT-PCR assay to quantify the expression of fum1 and fum19 genes from the Fumonisin-producing Fusarium ...its ...
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Validation and utilization of an internally controlled multiplex Real time RT PCR assay for simultaneous detection of enteroviruses and enterovirus A71 associated with hand foot and mouth disease
... obtained PCR amplicon [13], 2) it is a WHO recommended assay for diagnostics of HFMD pa- tients [19], and 3) it has been widely implemented in diagnostic laboratories [20, ...the assay described here ...
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Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... the real-time RT-PCR assay, since this strain is representative of H5 clade 3 viruses, which have not been detected since 1997, and can easily be distinguished genetically from H5 ...
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Improved Safety for Molecular Diagnosis of Classical Rabies Viruses by Use of a TaqMan Real Time Reverse Transcription PCR “Double Check” Strategy
... TaqMan real-time ...probe RT-PCR kit (Qiagen, Hilden, Germany) or the RNA UltraSense one-step quantitative RT-PCR sys- tem (Invitrogen, Carlsbad, ...The real-time ...
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Quantification of Hepatitis Delta Virus RNA in Serum by Consensus Real Time PCR Indicates Different Patterns of Virological Response to Interferon Therapy in Chronically Infected Patients
... the PCR efficiency was repeatedly over 93%. The quantitative real-time RT-PCR assay that was developed in our laboratory aimed to replace the qualitative assay as a ...
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Diagnosis of Human Metapneumovirus Infection in Immunosuppressed Lung Transplant Recipients and Children Evaluated for Pertussis
... effect. Real-time RT-PCR has been described previously for rapid detection of hMPV RNA (5, ...a real-time RT-PCR assay that is designed to detect the N gene ...
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Real Time Reverse Transcription PCR Assay for Comprehensive Detection of Human Rhinoviruses
... a real-time reverse transcription-PCR (RT-PCR) assay targeting the viral 5 ⴕ noncoding region defined by sequences obtained from all 100 currently recognized HRV prototype ...
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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS- CoV) to investigate the potential of a hot start RT for improved ...
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... The real-time RT-PCR assay was performed using the real- time one step RT-PCR master mix (Applied Biosystems International, Foster City, ...The ...
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External Quality Assessment for Enterovirus 71 and Coxsackievirus A16 Detection by Reverse Transcription PCR Using Armored RNA as a Virus Surrogate
... commercial real-time RT-PCR kits (PCR assays A to E), one triplex commercial real-time RT- PCR assay (PCR assay F), and one in-house ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... including RT-PCR and mPCR/ RLB, do not depend on the presence of viable or grow- ing bacteria, and thus are suitable to the detection of pathogens that cannot be cultured readily by routine methods, or that ...
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Classification and risk stratification of invasive breast carcinomas using a real time quantitative RT PCR assay
... correlation. Real-time qRT-PCR is attractive for clinical use because it is fast, reproducible, tissue-sparing, quantitative, automatable, and can be performed from archived (formalin-fixed, ...
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Concurrent Genotyping and Quantitation of Cytomegalovirus gB Genotypes in Solid Organ Transplant Recipients by Use of a Real Time PCR Assay
... the RT-GQ-PCR assay were specific for each corresponding target sequence, and no cross-reactions were ...between RT-GQ-PCR and RFA was 100% with 24 samples. However, ...
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Evaluation and Validation of Real Time Reverse Transcription PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus
... and real-time LC RT-PCR is the absence of a gold ...conventional RT-PCR with our primers but positive by LC-RT-PCR with the same set of ...LC RT-PCR ...
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Preclinical Evaluation of Two Real Time, Reverse Transcription PCR Assays for Detection of the Severe Acute Respiratory Syndrome Coronavirus
... We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR RT-PCR assay targeting the open reading frame ORF 1b region of the se[r] ...
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Evaluation of a Multiplex Real Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001 2002 Influenza Season in Israel
... multiplex real-time reverse transcriptase PCR (RT-PCR) assay (which uses TaqMan chemistry) and evaluated it for its ability to detect and concomitantly differentiate influenza ...
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Simultaneous Detection, Subgrouping, and Quantitation of Respiratory Syncytial Virus A and B by Real Time PCR
... TaqMan RT-PCR assay was evaluated by testing the method against viral RNAs de- rived from various ...TaqMan PCR, the results showed that all of the RSV strains and none of the other viruses ...
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Original Article Purpurin inhibits the adhesion of Candida albicans biofilms
... reduction assay and crystal violet assay as well as real time RT-PCR were used to detective the expression of genes ALS1, EFG1 and ...
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