recombinant adenoviruses
Recombinant adenoviruses with large deletions generated by Cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo.
... Currently, recombinant adenoviruses are one of the more efficient viral vehicles for transferring genes into nondividing cells in ...E1-deficient adenoviruses were used for gene therapy on the basis ...
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Influence of E1-Deleted Recombinant Adenoviruses on B7.1 and IL-2 Expression in C1498 Cells
... Background: Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1∆) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of ...
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Recombinant Adenoviruses Displaying Matrix 2 Ectodomain Epitopes on Their Fiber Proteins as Universal Influenza Vaccines
... recover recombinant adenoviruses, pAdC68-F3M2e(H1-H5-H7) or pAdC68-F3M2e(H1-H7-H5) clones were transfected into HEK293 cells with X-treme Gene HP DNA transfection reagent (Roche, Indianapolis, ...
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Cellular and humoral immune responses to viral antigens create barriers to lung-directed gene therapy with recombinant adenoviruses.
... first-generation recombinant adenoviruses is that deletion of E1 sequences is insufficient to completely ablate expression of other early and late viral genes or to prevent replication of viral ...
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Comparison of the protective efficacy between single and combination of recombinant adenoviruses expressing complete and truncated glycoprotein, and nucleoprotein of the pathogenic street rabies virus in mice
... fixed 2 days post-infection and subjected to antibody staining using RABV G and N protein-specific anti- bodies for IFA. The 293A cells infected with each re- combinant adenovirus produced specific fluorescence, while no ...
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Breast cancer selective gene expression and therapy mediated by recombinant adenoviruses containing the DF3/MUC1 promoter
... recombinant adenoviral vectors, containing the DF3 promoter (bp -725 to +31), can be used to express beta-galactosidase (Ad.DF3-betagal) and the herpes simplex virus thymidine kinase (HSV-tk) gene (Ad.Df3-tk) in ...
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Infection of eucaryotic cells by helper-independent recombinant adenoviruses: early region 1 is not obligatory for integration of viral DNA.
... Southern transfer experiments revealed that i the sites of integration in the host genome were not unique; ii in general, transformed CV1 cell lines contained single-copy, full-length vi[r] ...
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Fiberless Recombinant Adenoviruses: Virus Maturation and Infectivity in the Absence of Fiber
... Adenoviruses are characterized by a number of features that make them attractive tools for in vivo gene therapy (32). In particular, they are able to efficiently infect most quiescent and dividing human cells. ...
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Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro
... Infection with the Ad5-SVR4 virus was used to introduce the large T antigen encoding region of the SV40 virus into bovine and human corneal endothelial cells.. Expression of large T anti[r] ...
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Cell-specific expression of artificial microRNAs targeting essential genes exhibit potent antitumor effect on hepatocellular carcinoma cells
... a recombinant promoter comprised of human α-fetoprotein enhancer and basal ...three recombinant adenoviruses showed efficient blockage of cell cycle progression and significant suppression of HCC ...
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Overlap in signaling between Smoothened and the α subunit of the heterotrimeric G protein G₁₃
... We should not have been surprised at the poor efficiency of transduction for C3H10T1/2 cells: MOIs reported for this cell line are not uncommonly 100, and few studies report the per- cent of cells actually transduced ...
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Future of adenoviruses in the gene therapy of arthritis
... Recombinant adenoviruses are straightforward to produce at high titres, have a promiscuous host- range, and, because of their ability to infect nondividing cells, lend themselves to in vivo gene ...
5
Adenoviruses in Gene Therapy A Review
... To set off transcription of the induced transgene, this DNA sequence has to have a promoter gene in front (upstream) on the DNA strand. Very promising preclinical studies have concentrated on regulation of the promoter. ...
5
Recombinant Human Adenovirus: Targeting to the Human Transferrin Receptor Improves Gene Transfer to Brain Microcapillary Endothelium
... with recombinant viral vectors, with secretion of enzyme product basolaterally, could allow for wide- spread enzyme ...for recombinant adenovirus ...that adenoviruses can be modified in the HI loop ...
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Biophysical Comparison of Two Canine Adenoviruses
... The results of the trypsin digestion experiments Table 1, in which the rapidly sedimenting incomplete soluble hemagglutinin and the slowly sedimenting incomplete soluble hemagglutinin we[r] ...
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Replication selective adenoviruses as oncolytic agents
... A second strategy for optimizing tumor selectivity is to delete gene functions that are critical for efficient viral replication in normal cells but not in tumor cells. Many of the same critical regulatory proteins that ...
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Hybrid of Monkey and Human Adenoviruses
... The hybrid virions multiplied in green monkey kidney cells in the presence of human adenovirus types 1, 2, and 3, but not 3 and 7, and acquired the capsid of the helper adenovirus.. The [r] ...
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Adenoviruses with nonidentical terminal sequences are viable.
... In a second attempt to isolate pure plaques with AdS left and right termini or with Ad2 left termini and Ad5 right termini, the sample sub54-21 with a composite signal was plaque purifie[r] ...
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Neutralization of adenoviruses: kinetics, stoichiometry, and mechanisms.
... Neutralization of virions attached to HeLa cells % Neutralization when the following amt ,ul of antiserum was added to the virus-cell mixture' 25 50 250 500 Antiserum treatment .94 0 13 [r] ...
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Enteric adenoviruses: detection, replication, and significance
... In addition to the 42 stools with adenovirus particles, 15 other stool specimens from gastroenteritis patients that either had no virus detectable by EM or contained rotavirus or picorna[r] ...
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