Recombinant antigen
Novel Recombinant Antigen Enzyme Immunoassay for Serological Diagnosis of Syphilis
... tal wards and clinics including genitourinary-medicine wards and antenatal clinics was comparable to that found by other recombinant-antigen EIAs evaluated with specimens from more restricted populations. ...
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Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis
... the recombinant antigen B8/1 (rAgB) in an enzyme-linked immunosorbent assay (ELISA) format; however, in remote parts of areas where the disease is endemic, the implementation of an ELISA is difficult, so a ...
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Recombinant Antigen Targets for Serodiagnosis of African Swine Fever
... of recombinant protein-based ELISA with poorly preserved ...new recombinant ELISAs to such sera, 39 field serum samples previously classified to be ASFV positive by OIE- approved tests were kept at 37°C for ...
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Use of MAG1 Recombinant Antigen for Diagnosis of Toxoplasma gondii Infection in Humans
... cyst and in the cyst wall surrounding the bradyzoites. We tested the reactivity of MAG1 using two different groups of human sera that were characteristic for presumed acute and chronic infections, respectively. Both ...
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Immediate-Early Expression of a Recombinant Antigen by Modified Vaccinia Virus Ankara Breaks the Immunodominance of Strong Vector-Specific B8R Antigen in Acute and Memory CD8 T-Cell Responses
... Homologous prime/boost immunizations using a single pox- viral vector have frequently been disfavored due to reports describing inefficient cytotoxic T-lymphocyte (CTL) induction under conditions of preexisting ...
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Serodiagnosis of Recently Acquired Toxoplasma gondii Infection with a Recombinant Antigen
... Construction of P35 fusion proteins. The DNA sequence of the gene encoding a 35-kDa antigen (P35) from T. gondii was obtained from the GenBank database (accession number A19564) and the Toxoplasma EST database ...
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Immunologic characterization of a 35 kilodalton recombinant antigen of Mycobacterium tuberculosis
... tuberculosis sonic extract C, and purified recombinant protein D compared by silver staining lanes 1 and Western blot immunoblot reacted with monoclonal antibody 2B2 lanes 2.. mw, Molecu[r] ...
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Enzyme-Linked Immunosorbent Assay for Detection of Plasmodium falciparum Histidine-Rich Protein 2 in Blood, Plasma, and Serum
... Assay procedure and linear range of the HRP2 ELISA. A commercial PfHRP2 ELISA kit (Malaria Ag CELISA; Cellabs Pty. Ltd., Brookvale, New South Wales, Australia) was used with modifications (H. G. Rajasekariah, personal ...
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Serological Method Using Recombinant S2 Protein To Differentiate Equine Infectious Anemia Virus (EIAV)-Infected and EIAV-Vaccinated Horses
... S2 antigen from E. coli. In parallel with our development of an S2 antigen expression system in mammalian COS7 cells as a basis for investigating S2 function in viral replication, we also examined the ...
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Simultaneous Detection of Antibodies to Mouse Hepatitis Virus Recombinant Structural Proteins by a Microsphere-Based Multiplex Fluorescence Immunoassay
... each antigen varies among individuals or strains of animals as well as virus strains, and consequently, multiplex analysis using polyvalent antigens is recommended in recombinant antigen-based ...
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Enzyme linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus
... The recombinant antigen sequence of rE2 of CHIKV (strain DRDE-06), comprising 1167 nucleotides (nt) in length, without the transmembrane region (Genbank ac- cession number MG945127), was cloned into a ...
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Recombinant Hepatitis A Virus Antigen: Improved Production and Utility in Diagnostic Immunoassays
... HAV antigen to detect HAV-specific anti- ...HAV antigen in tissue culture is time-consuming and ...in recombinant vaccinia viruses ...HAV antigen by standard tissue culture methods to the ...
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Expression of the Bovine Leukemia Virus Envelope Glycoprotein (gp51) by Recombinant Baculovirus and Its Use in an Enzyme-Linked Immunosorbent Assay
... ELISA with rgp51. The ELISA with rgp51 was performed as described previ- ously (7), with some modification. Briefly, ELISA plates (MaxiSorp; Nalgene Nunc International) were coated with a predetermined optimal quantity ...
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Cloning and Expression of a DNA Sequence Encoding a 41-Kilodalton Cryptosporidium parvum Oocyst Wall Protein
... Recombinant CP41 polyhistidine fusion protein was purified by denaturing Ni-nitrilotriacetic acid (NTA) affinity chromatography in accordance with the manufacturer’s (Invitrogen) directions. Eluates containing ...
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Original Article Construction, expression and purification of recombinant HBcAg-MAGE-A3 therapeutic cancer vaccine
... at the amino terminus of HBcAg form a spike tip, the major immunodominant region (MIR), foreign epitopes could be inserted into MIR to generate a fusion peptide. This region was pre- viously reported to be the major B ...
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<p>Development, optimization, and validation of an in-house Dot-ELISA rapid test based on SAG1 and GRA7 proteins for serological detection of <em>Toxoplasma gondii</em> infections</p>
... the recombinant antigen concentrations, dilution of serum samples and secondary antibody ...each antigen used in the test was assessed using four concentrations of antigens ...
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Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test.
... the recombinant antigen were 90% and 86%, respectively. This antigen showed no cross-reactivity with sera of patients infected with Hydatid cyst, HCV, Epstein barr virus, HBV, Helicobacter pylori, ...
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KBMA Listeria monocytogenes is an effective vector for DC mediated induction of antitumor immunity
... melanoma-associated antigen, delivered the antigen for presentation by MHC class I and class II molecules independent of the MHC haplotype of the DC ...deliver recombinant antigen to DCs for ...
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An Overview on Leishmania Diagnosis
... HIV/AIDS patients [108]. The diagnostic principles remain essentially the same as those for non-HIV-infected patients. Amastigotes may exist in buffy coat and sometimes may be found in unusual locations, such as pleural ...
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Study of the Functional Activities Concomitantly Retained by the 115,000 Mr Super T Antigen, an Evolutionary Variant of Simian Virus 40 Large T Antigen Expressed in Transformed Rat Cells
... Transformation of secondary rat kidney cell cultures by recombinant DNA containing the super T antigen gene SVE 5 Kb and pSVsT or the large T antigen gene pSV1 and pSV40' DNA tested Plas[r] ...
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