recombinant antigens
Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies
... on recombinant GRA6 than for the Euroimmun avidity test (Euroimmun, Lübeck, Germany) for the exclusion of recent in- fection, occurring less 4 months previously, in pregnant ...of recombinant proteins, ...
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Seroreactivity to and Avidity for Recombinant Antigens in Toxoplasmosis
... applied at 1:100 dilutions and incubated together with the nitrocellulose test strips for 1 h at room temperature. Following three washing steps of 5 min each, a second incubation of 45 min with peroxidase-labeled ...
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Use of Recombinant Antigens for Early Postnatal Diagnosis of Congenital Toxoplasmosis
... Next, we examined the immunoreactivity of recombinant antigens with immunoglobulin M of infant sera. To this aim, a double-sandwich immunoassay was employed (IgM Rec- ELISA). The GST-MIC2a, GST-MIC2b, ...
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Evaluation of Recombinant Antigens for Serodiagnosis of Chagas’ Disease in South and Central America
... years, recombinant DNA technology has been used to isolate and characterize ...cruzi antigens which are immunodominant in human infections (4, 6, 7, 10, 14, ...defined antigens has shown promising ...
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Usefulness of Four Different Echinococcus granulosus Recombinant Antigens for Serodiagnosis of Unilocular Hydatid Disease (UHD) and Postsurgical Follow-Up of Patients Treated for UHD
... Finally, 71 sera from 35 UHD patients subjected to and cured by surgical intervention were recovered to monitor the level of specific UHD antibodies for 11 years. These sera were classified into six groups as follows: ...
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Western Immunoblotting with Five Treponema pallidum Recombinant Antigens for Serologic Diagnosis of Syphilis
... the recombinant form have been used in enzyme immunoassay methods (9, 12, 19, 48, 50), only a few preparations of recombinant ...of recombinant antigens could avoid the difficulties in ...
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Protection of ewes against Teladorsagia circumcincta infection in the periparturient period by vaccination with recombinant antigens
... the antigens during grazing on contaminated pasture and again during the trickle infection period, but not to the extent of that observed in the vaccinates (Supplementary data ...S1D). Recombinant ...
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Recombinant antigens for immunodiagnosis of cystic echinococcosis
... (HCF) antigens are the usual source of antigenic material for immunodiagnostic assays ...with antigens from other parasites, notably other taeniid cestodes, is a major problem when using HCF antigens ...
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Large scale screening of human sera with cytomegalovirus recombinant antigens
... DISCUSSION To evaluate whether one or more HCMV recombinant proteins could be used to detect anti-HCMV antibodies in serological procedures, 10 different recombinant proteins containing [r] ...
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Serum IgE and IgG reactivity to Aspergillus recombinant antigens in patients with cystic fibrosis
... 32 Methodology: Serum IgE and IgG antibodies were measured in 12 CF patients with Allergic Bronchopulmonary 33 Aspergillosis ABPA, 12 with Aspergillus sensitization AS, and 12 with Asper[r] ...
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Multiplex Screening of Surface Proteins from Mycoplasma mycoides subsp. mycoides Small Colony for an Antigen Cocktail Enzyme-Linked Immunosorbent Assay
... A recombinant antigen cocktail enzyme-linked immunosorbent assay (ELISA) for diagnosis of contagious bovine pleuropneumonia (CBPP) was developed after careful selection of antigens among one-third of the ...
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Multi antigen print immunoassay (MAPIA) based evaluation of novel recombinant Leishmania infantum antigens for the serodiagnosis of canine visceral leishmaniasis
... these antigens are mainly detectable in cases with advanced stages of disease, while they are much less detectable in sera from asymptomatic cases ...the recombinant antigens evaluated were more ...
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EFFORTS TOWARDS THE DEVELOPMENT OF RECOMBINANT VACCINES AGAINST PASTEURELLA MULTOCIDA
... Hemorrhagic septicemia is caused by gram-negative bacterium of Pasteurella multocida (P. multocida) strains. Most of the current vaccines against P. multocida have shortcomings. Presently, there is increasing efforts ...
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Detection of cytomegalovirus antibodies by an enzyme linked immunosorbent assay using recombinant polypeptides of the large phosphorylated tegument protein pp150
... More detailed analysis using a combination of the recombinant antigens from ppl50 in ELISAs and evaluation of the results with other antibody test systems will show whether recombinants [r] ...
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COST 820 Vaccines against animal coccidioses (4th) Annual report 1998 EUR 19223 EN
... Under the umbrella title "Recombinant antigens and other tools for the development of molecularengineered vaccines against coccidial parasites" six papers from WG4 demonstrated the very [r] ...
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An evaluation of a recombinant multiepitope based antigen for detection of Toxoplasma gondii specific antibodies
... new antigens developed in this work was achieved at the maximum values of ...several recombinant antigens developed recently for the diagnosis of toxo- plasmosis [3, ...
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Serum Antibody Responses to 10 Mycobacterium tuberculosis Proteins, Purified Protein Derivative, and Old Tuberculin in Natural and Experimental Tuberculosis in Rhesus Monkeys
... purified recombinant antigens, PPD, and OT in rhesus monkeys experimentally infected with Mycobacterium tuberculosis and analyzed the time to antibody detection, antibody levels, and their association with ...
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Gao, Jinliang (2009): Molecular characterization of the fibronectin-binding protein BBK32 of Borrelia burgdorferi sensu lato. Dissertation, LMU München: Medizinische Fakultät
... the recombinant immunoblot this is achieved by selection of the respective specific recombinant ...Europe, recombinant immunoblots with quite high sensitivity and specificity have been developed by ...
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Serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens
... A sandwich enzyme-linked immunosorbent assay with monoclonal antibodies against EM10 and EG55 as capture reagents for the recombinant antigens was established and was evaluated with 74 s[r] ...
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Original Article Cloning and characterization of Clp protease proteolytic subunit 2 and its implication in clinical diagnosis of tuberculosis
... involve recombinant Mtb proteins produced under various growth conditions, which can be detected in vitro or in vivo at different stages of ...few antigens are available, and a systematic study on Mtb ...
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