Recombinant baculoviruses
Use of recombinant baculoviruses in synthesis of morphologically distinct viruslike particles of flock house virus, a nodavirus.
... Here, we show that Spodoptera frugiperda cells infected with recombinant baculoviruses containing the gene for wild-type protein alpha synthesized high yields of viruslike particles with[r] ...
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Insecticidal activity of two proteases against Spodoptera frugiperda larvae infected with recombinant baculoviruses
... Recombinant baculoviruses have also been constructed with enhancin genes from other ...and baculoviruses themselves also pro- duce chitinases to liquefy the host body after their death by viral ...
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Herpes simplex virus capsids assembled in insect cells infected with recombinant baculoviruses: structural authenticity and localization of VP26.
... Recently, recombinant baculoviruses have been used to show that expression of six herpes simplex virus type 1 genes results in the formation of capsid-like ...
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In vivo and in vitro analyses of recombinant baculoviruses lacking a functional cg30 gene.
... Furthermore, restriction endonuclease digestion of AcMNPV DNA with SfiI which inactivates cg3O and another AcMNPV gene, pl43, does not affect transient expression from the etl, vp39, or [r] ...
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Expression and secretion of the bovine coronavirus hemagglutinin-esterase glycoprotein by insect cells infected with recombinant baculoviruses.
... However, murine hepatitis virus MHV strain A59 contains an open reading frame with sequence similarity to both the HE gene of BCV and the HE gene of type C influenza viruses 17, and a va[r] ...
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Expression of the major capsid protein VP6 of group C rotavirus and synthesis of chimeric single-shelled particles by using recombinant baculoviruses.
... 10 0022-538X/92/105825-07$02.00/0 Copyright © 1992, American Society for Microbiology Expression of the Major Capsid Protein VP6 of Group C Rotavirus and Synthesis of Chimeric Single-She[r] ...
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Assembly of herpes simplex virus (HSV) intermediate capsids in insect cells infected with recombinant baculoviruses expressing HSV capsid proteins.
... These and other observations indicate that i the products of the UL18, UL19, UL35, and UL38 genes self-assemble into structures that form the outer surface icosahedral shell of the capsi[r] ...
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Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10.
... To demonstrate that BTV-10 NS1 protein was synthesized in recombinant baculovirus-infected cells, protein extracts were prepared from insect cells infected with the recombinant virus AcB[r] ...
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The bovine herpesvirus 1 maturational proteinase and scaffold proteins can substitute for the homologous herpes simplex virus type 1 proteins in the formation of hybrid type B capsids.
... a recombinant baculovirus expressing the BHV-1 ...with recombinant baculoviruses expressing the BHV-1 UL26- and ...with recombinant baculoviruses expressing the BHV-1 UL26-encoded ...
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Identifying Epitopes Responsible for Neutralizing Antibody and DC-SIGN Binding on the Spike Glycoprotein of the Severe Acute Respiratory Syndrome Coronavirus
... a recombinant baculovirus, vAtEpGS763, containing partial SARS-CoV S protein (aa residues 17 to 763) was used to study its interaction with ...recombinant baculoviruses. (A) Direct infection assay. A ...
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Functional Analysis of the Triplex Proteins (VP19C and VP23) of Herpes Simplex Virus Type 1
... The capsid proteins were expressed in recombinant baculoviruses using the BAC-to-BAC system (Invitrogen) (13). The transfer vector pFastBacHta (pFBHta) was used to express VP5, VP19C, and UL80HSVCT (a ...
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Glycoprotein H of herpes simplex virus type 1 requires glycoprotein L for transport to the surfaces of insect cells.
... Three recombinant baculoviruses, expressing gL1, gH1, and truncated gH1 (gH1t), which lacks the transmembrane region, were ...that recombinant gH1/gL1 and gH1t/gL1 heterooligomers were produced in ...
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The Interaction between the Major Capsid Protein and the Smallest Capsid Protein of Human Cytomegalovirus Is Dependent on Two Linear Sequences in the Smallest Capsid Protein
... recombinant baculoviruses encoding the ...the recombinant baculovirus en- coding the MCP was loaded directly on the gel and after the lysate was transferred to membranes ...a recombinant ...
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Mutational Analysis of the N-Linked Glycans onAutographa californica Nucleopolyhedrovirus gp64
... FIG. 6. Carbohydrate compositions of individual N-linked glycans on AcM- NPV gp64. Sf9 cells were infected at a multiplicity of about 0.01 PFU per cell with wild-type AcMNPV (WT) or recombinant ...
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Expression of rotavirus VP2 produces empty corelike particles.
... Cotransfection of Spodoptera frugiperda 9 cells with wild-type baculovirus DNA and transfer vector DNA led to the formation of recombinant baculoviruses which contain bovine rotavirus ge[r] ...
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In vitro resolution of adeno-associated virus DNA hairpin termini by wild-type Rep protein is inhibited by a dominant-negative mutant of rep.
... We expressed both wild-type Rep78 and mutant Rep78NTP proteins in two helper-free expression systems consisting of either recombinant baculoviruses in insect cells or the human immunodef[r] ...
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A 96 well format for a high throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
... is determined by detecting morphological changes in infected cells using laborious and time-consuming plaque formation and/or end-point dilution assays [7]. Several improved protocols for virus titering have been ...
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Synthesis and characterization of chimeric particles between epizootic hemorrhagic disease virus and bluetongue virus: functional domains are conserved on the VP3 protein.
... Electron micrographs of double-shelled EHDV chimeric VLPs synthesized in insect cells by three recombinant baculoviruses: one expressing the EHDV-1 VP3 core protein, one expressing the B[r] ...
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The Full Length Hepatitis C Virus Polyprotein and Interactions with the Interferon-Beta Signalling Pathways in vitro
... Fig. 1. The schematic of structures of pBac H77 (HDV) Tet and pBac J4 (HDV) Tet. (A), The schematic of a pBacMam-2 ampicillin resistance vector containing the whole genome of HCV1a (H77) donated by Dr. Christopher ...
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Antibody recognition of the glycoprotein g of viral haemorrhagic septicemia virus (VHSV) purified in large amounts from insect larvae
... The recombinant G21-507 gene under the control of the polyhedrin promoter, using an insect signal sequence derived from honey bee melitin and adding a carboxy terminal poly histidine (polyHis) tag for affinity ...
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