Recombinant protein: Cloning and expression
Gene cloning and recombinant protein expression of lipase from marine bacterium
... June 2014 Chairman : Adam Leow Thean Chor, PhD Faculty : Biotechnology and Biomolecular Sciences Nowadays salt tolerant enzymes have gained attention since they have advantages in food processing industries and ...
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Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata
... Smsp-4 expression varied seasonally and tended to be higher in summer and lower in winter, suggesting that Smsp-4 plays more important roles in summer than in ...MDa protein complex, comprising six sets of ...
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Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
... prolonged expression of GRA proteins make them one of the promising candidates for recombinant protein ...that recombinant production of sev- eral Toxoplasma antigens, such as dense granule ...
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Helicobacter pylori recombinant UreG protein: cloning, expression, and assessment of its seroreactivity
... Methods: The coding sequence of H. pylori UreG was cloned and the recombinant protein expressed and purified by affinity chromatography using nickel nitrilotriacetic acid (Ni-NTA) resin. The antigenicity of ...
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Cloning, Expression and Characterization of Recombinant Exotoxin A-Flagellin Fusion Protein as a New Vaccine Candidate against Pseudomonas aeruginosa Infections
... the recombinant fusion protein in 1:2000 ...recombinant protein. These results also indicate that this fusion protein may be used as a serodiagnostic antigen for rapid diagnosis of ...
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Cloning and Expression of a 48 KilodaltonBabesia caballi Merozoite Rhoptry Protein and Potential Use of the Recombinant Antigen in an Enzyme Linked Immunosorbent Assay
... cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of ...48-kDa protein of ...The recombinant ...
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Cloning, expression and characterization of gE protein of Duck plague virus
... denatured protein was done by gradient dialysis in ...purified recombinant protein ...purified recombinant protein ...purified recombinant protein ...
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Cloning and characterization of two recombinant Neospora protein fragments and their use in serodiagnosis of bovine neosporosis.
... as recombinant proteins for the development of an enzyme-linked immunosorbent assay ...pRSET expression vector. Antibodies to recombinant protein N54 recognized five major bands from a ...
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Cloning and expression of recombinant human granulocyte-macrophage colony stimulating factor (rhGMCSF) in Escherichia coli
... an expression host because of its special ...the protein structure, and increase production ...for protein expression in ...the expression of recombinant protein and ...
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Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein
... CSUI_005805 protein was cloned into the bacterial expression vector pQE-31 to express the recombinant protein rCSUI_005805 in a size similar to the predicted molecular mass that was present in ...
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Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... fusion protein. To further improve protein solubility the polar amino acids lysine and glutamine can also be used in the ...of protein solubility prediction online software, the solubilities of HspX ...
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Cloning and Expression of Recombinant Proteins
... Restriction-enzyme based cloning PCR DNA Sequencing Yeast genetics Eukaryotic protein expression systems Transfection of cell culture cells. Insect cell expression[r] ...
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Cloning and Expression of a Helicobacter bilis Immunoreactive Protein
... Thus, despite the logic of focusing on membrane proteins as potential antigens for serodiagnosis, other antigens may be superior in terms of antigenicity, species specificity, and possi- bly sensitivity. Comparison of ...
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Cloning, Expression, and Immunological Characterization of the P30 Protein of Mycoplasma pneumoniae
... Bacterial cells were collected by centrifugation and were further subjected to sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) to analyze the expression of the recombinant proteins. ...
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Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells
... attractive expression system for the manufacture of therapeutic ...folded recombinant proteins with post-translational ...for protein production in the pharmaceutical industry, we selected this cell ...
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Cloning and Expression of Coxsakievirus B3 Viral Protein-1 in E. Coli
... this protein facilitates the binding of antibody molecules to VP1, and hence VP1 can provoke the immune system of the body ...with recombinant VP1 were protected from the virus infection when ...the ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... The recombinant AcNPV-VP4 DNA was transfected by lipofection into the insect cell line, Spodoptera frugiperda (Sf9) ...cells. Expression of VP4 in the Sf9 cells was confirmed by the immunofluorescence test ...
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Cloning, Phylogenetic Analysis and Expression of Recombinant LipL41, Loa22 and LipL21 Proteins from Leptospira interrogans
... develop recombinant outer membrane protein based diagnostic ...For protein expression truncated lipL41 (1028 bp), loa22 (548bp) and lipL21 (472bp) genes were amplified, cloned and expressed in ...
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Cloning of Aspergillus Niger BglA and expression of recombinant β-glucosidase in methylotrophic yeast Pichia Pastoris
... allow protein engineering work to be performed to improve the properties of this enzyme, it is important to clone and sequence gene encoding this enzyme from different isolates and produce recombinant ...
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Cloning and Expression of Recombinant Plasmid Containing P36/LACK Gene of Leishmania infantum Iranian Strain.
... Accepted 12 Jan 2015 Abstract Background: There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular ...
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