Recombinant protein production
Pichia pastoris: a workhorse for recombinant protein production
... for recombinant protein production in ...the recombinant proteins are produced under the control of the AOX1 promoter in Mut + or Mut S ...
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Protein Body Biogenesis and Utility in Recombinant Protein Production in Nicotiana benthamiana
... and recombinant protein accumulation levels in the process of PB formation, and to investigate the potential utility of PBs as a tool to facilitate recombinant protein production in ...
153
Whole synthetic pathway engineering of recombinant protein production
... optimal, protein-specific ratios of biosynthetic process rates and/or polypeptide chain expression ...and/or recombinant protein production per cell (Allen et ...maximizing recombinant ...
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Side effects of chaperone gene co-expression in recombinant protein production
... proper protein folding in recombinant protein ...folded, recombinant proteins in bacterial cell ...folding-reluctant protein species might instead trigger undesired proteolytic ...
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Side effects of chaperone gene co-expression in recombinant protein production
... the recombinant protein ...target recombinant proteins [43-45] and solubility has been often enhanced at expenses of protein yield ...the recombinant protein ...target ...
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A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli
... the recombinant protein downstream of a strongly-regulated T7 ...in recombinant protein production settings based on available ...repressor protein. The TetR protein [22] ...
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Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production
... heterologous protein expression and to understand diploid strain sta- bility in high-productivity ...target protein, we demonstrate that recombinant protein production in both wild-type ...
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Stable overexpression of miR-17 enhances recombinant protein production of CHO cells
... In the past decade miRNA research has gained much attention in biology and found broad applications including diagnostics, thera- peutics and cell engineering ( Barron et al., 2011b; Bratkovic et al., 2012; Hackl et al., ...
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Smart sustainable bottle (SSB) system for E. coli based recombinant protein production
... Protein production in the SSB system (2-L) using the de- fined autoinduction medium (S-DAB (HNC)) leads to equal results concerning final product yields and solubil- ity as reached in conventional ...
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A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris
... for recombinant protein pro- duction, which is usually regulated either by a constitutive promoter ...regulating recombinant expression of one or more genes were ...tunable recombinant ...
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Enhancing recombinant protein production in human cell lines with a constitutive transport element and mRNA export proteins.
... transient recombinant protein production in human cell lines, precise comparisons could not be made because the overexpression of Tap-p15 per se enhanced gene expression in general, including that of ...
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High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor
... for recombinant protein production in rocking-motion-type ...successful production of many proteins, the challenge was to develop a strategy which is robust enough so that it also can be used ...
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Thermal adaptability of Kluyveromyces marxianus in recombinant protein production
... folded recombinant proteins ...target protein in a soluble state consists in evaluating different growth tem- ...the production rate, allowing the newly synthesized chain to fold properly ...
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Comparing the Recombinant Protein Production Potential of Planktonic and Biofilm Cells
... Keywords: green fluorescent protein; Escherichia coli; biofilm; planktonic; flow cell reactor 22.. 23.[r] ...
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Identification and characterization of cysteine protease genes in tobacco for use in recombinant protein production
... 32 Chapter 3: Results 3.1 Tobacco genome contains at least 60 putative CysPs To identify CysP genes in tobacco, an in silico search was done using the DFCI tobacco gene index (TGI) database. The TGI consists of 324,058 ...
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Development of tools and methods for genetic engineering of the recombinant protein production host Pichia pastoris
... on protein secretion, whereas the beneficial effect of rim101∆ and sgt2∆ depends on the secreted ...encoded protein was responsible for the observed secretion enhancement, we decided to express the ...
226
Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli
... expression levels [3,4]. The presence of rare codons, which are correlated with low levels of their cognate tRNAs species in the cell, can reduce the translation rate and induce translation errors with a significant ...
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Trends in recombinant protein use in animal production
... and protein engineering techniques have led to a significant progress in animal produc- tion and it is starting to have a commercial impact in this ...functional protein [181]. On the other hand, this ...
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Production of recombinant protein by a novel oxygen-induced system in Escherichia coli
... for recombinant protein production in ...or recombinant protein recovery and purification processes ...induce recombinant gene expression by in- creasing the molecular oxygen ...
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Recombinant IgM expression in mammalian cells: A target protein challenging biotechnological production
... 3. RECOMBINANT EXPRESSION OF IgM ...for recombinant protein production are Chi- nese hamster ovary (CHO) ...antibody production that typically have specific produc- tivities in the ...
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