Recombinant tumor necrosis factor
Effects of recombinant tumor necrosis factor on proliferation and differentiation of leukemic and normal hemopoietic cells in vitro Relationship to cell surface receptor
... The clonogenic growth of myeloid leukemia cell lines was inhibited by recombinant tumor necrosis factor (rTNF) at 1-15 pM concentration. However, wild type (promyelocytic) HL-60 cells were ...
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Development of partial tolerance to the gastrointestinal effects of high doses of recombinant tumor necrosis factor alpha in rodents
... gastrointestinal inflammation. Within 30 min gastric emptying was blocked and tissue edema occurred in the small and large intestine. In the cecum hemorrhage occurred after 4 h at doses greater than or equal to 250 ...
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Recombinant tumor necrosis factor/cachectin and interleukin 1 pretreatment decreases lung oxidized glutathione accumulation, lung injury, and mortality in rats exposed to hyperoxia
... factor/cachectin (TNF/C) and interleukin-1 (IL-1) prolonged the survival of rats (144 +/- 9 h) in continuous hyperoxia (greater than 99% O2 at 1 atm) when compared with rats injected with boiled TNF/C and boiled ...
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Interleukin-2 and alpha/beta interferon down-regulate hepatitis B virus gene expression in vivo by tumor necrosis factor-dependent and -independent pathways.
... 1265 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest We have recently reported that administration of recombinant tumor necrosis factor alpha TNF-a to hepatitis B virus [r] ...
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Tumor necrosis factor and immune interferon synergistically induce cytochrome b 245 heavy chain gene expression and nicotinamide adenine dinucleotide phosphate hydrogenase oxidase in human leukemic myeloid cells
... Recombinant tumor necrosis factor (rTNF) and rIFN-gamma induce in the human leukemia cell lines HL-60, ML3, and U937 the accumulation of transcripts of the X chromosome- linked chronic ...
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Treatment with recombinant human tumor necrosis factor alpha protects rats against the lethality, hypotension, and hypothermia of gram negative sepsis
... of recombinant human TNF-alpha (100 micrograms/kg) to rats undergoing CLP 1 d after the treatment period resulted in a significant reduction in mortality compared to control rats previously unexposed to rTNF (P ...
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Role of Eosinophils and Tumor Necrosis Factor Alpha in Interleukin 25 Mediated Protection from Amebic Colitis
... Recombinant IL-25 treatment. Mice were injected intraperitoneally with 0.5 g recombinant IL-25 (R&D Systems) or 100 l PBS each day for 4 days before and through 4 days after E. histolytica ...
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High levels of circulating soluble receptors for tumor necrosis factor in hairy cell leukemia and type B chronic lymphocytic leukemia
... immunoassays using nonblocking anti-receptor antibodies and 125I-recombinant human TNF alpha. As compared to the titers observed in 25 healthy controls, TNFBP types A and B titers were found to be elevated in ...
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Decreased progranulin levels in patients and rats with subarachnoid hemorrhage: a potential role in inhibiting inflammation by suppressing neutrophil recruitment
... and tumor necrosis factor- α (TNF- α ) were detected in the cerebrospinal fluid (CSF) from SAH patients by enzyme-linked immunosorbent assay ...(IHC). Recombinant human PGRN (r-PGRN) or an ...
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Effect of recombinant human tumor necrosis factor alpha on cerebral oxygen uptake, cerebrospinal fluid lactate, and cerebral blood flow in the rabbit: role of nitric oxide
... of tumor necrosis factor-alpha in the central nervous system in a rabbit ...of recombinant human TNF into the cisterna magna in the rabbit led to an acute reduction in cerebral oxygen uptake ...
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Tumor necrosis factor can induce fever in rats without activating protein breakdown in muscle or lipolysis in adipose tissue
... Tumor necrosis factor (TNF, cachectin) is a macrophage product that has been suggested to signal the loss of body weight, the decrease in adipose tissue and muscle mass, and anorexia during ...
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Recombinant human tumor necrosis factor alpha Regulation of N formylmethionylleucylphenylalanine receptor affinity and function on human neutrophils
... Abstract Preincubation of neutrophils with recombinant human tumor necrosis factor-a rH TNF-a enhanced the subsequent release of superoxide anion in response to various concentrations of[r] ...
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Recombinant human tumor necrosis factor alpha suppresses autoimmune diabetes in nonobese diabetic mice
... Among recombinant mouse IFN gamma, human IL1 alpha, human IL2, mouse granulocyte-macrophage colony-stimulating factor and human TNF alpha, only human TNF alpha suppressed insulitis and significantly (P less ...
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Regulation of human lung fibroblast glycosaminoglycan production by recombinant interferons, tumor necrosis factor, and lymphotoxin
... of recombinant (r) tumor necrosis factor (TNF), lymphotoxin (LT), and gamma, alpha, and beta 1 interferons (INF-gamma, -alpha and -beta 1), alone and in combination, on GAG production by ...
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Development of a Recombinant Xenogeneic Tumor Necrosis Factor Alpha Protein Vaccine To Protect Mice from Experimental Colitis
... the recombinant proteins were charac- terized by Western blotting assay with rabbit monoclonal anti-TNF- ␣ antibodies (CST, Danvers, MA, USA), and peptide mass fingerprinting were determined by matrix-assisted ...
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Cachectin/Tumor Necrosis Factor
... Clinical trials of recombinant human CachectinTrNF are underway. Phase-I studies confirmed many of the observations initially made in animals; profound systemic and organ specific toxicity has been observed, ...
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Expression of recombinant feline tumor necrosis factor is toxic to Escherichia coli.
... Mutations resulting in failure to fold or target the protein appropriately could lead to an accumulation of the fusion protein within the bacteria (29). Protein accumulation inter- feres with normal cell function and may ...
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Effect of Tumor Necrosis Factor on Body Systems
... the factor that may differ also in their biological ...%). Recombinant human proteins with deletions of 27 amino acids from the N-terminus appear to be biologically active in several ...
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The 55 kD tumor necrosis factor receptor on human keratinocytes is regulated by tumor necrosis factor alpha and by ultraviolet B radiation
... expression, indicating that TNF responsiveness of human keratinocytes critically depended on regulation of 55-kD TNFR expression. Human keratinocyte 55-kD TNFR surface and mRNA expression was found to be regulated in ...
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Effects of human interleukin 1 and human tumor necrosis factor on human T lymphocyte colony formation
... and tumor necrosis factor ...formation. Recombinant IL-1 and recombinant TNF were both shown to stimulate T cell colony growth in a dose-dependent manner, and this stimulation could be ...
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