recombinase polymerase amplification
Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
... Results: Recombinase polymerase amplification (RPA) assays for the detection of Bean golden yellow mosaic virus, Tomato mottle virus and Tomato yellow leaf curl virus (TYLCV) were specific, only ...
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Field Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp capripneumoniae
... Contagious caprine pleuropneumonia (CCPP) is a highly contagious disease caused by Mycoplasma capricolum subsp. capri- pneumoniae that affects goats in Africa and Asia. Current available methods for the diagnosis of ...
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Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids
... Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology that does not require any thermal cycling, as the amplifi- cation reaction is performed ...
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Development of real time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus
... transcription polymerase chain reactions (RT-PCRs) are widely used for detection of PPRV genomic ma- terial [14, ...thermal amplification (LAMP) provides an isothermal method to amplify viral RNA without ...
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Early Detection of Dengue Virus by Use of Reverse Transcription Recombinase Polymerase Amplification
... tion-recombinase polymerase amplification (RT-RPA) assay was ...isothermal amplification (RT-LAMP) assay, quantitative RT-PCR (qRT-PCR), and IgM- and IgG- capture enzyme-linked immunosorbent ...
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Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection
... programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but requires infrastructure, financial resources and skilled personnel, often not available in endemic ...areas. ...
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A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses
... desirable. Recombinase polymerase amplification (RPA), combined with lateral flow (LF) strips, is a novel approach to rapidly amplify and visualize ...
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Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents
... Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase ...
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Development of a fluorescent probe based recombinase polymerase amplification assay for rapid detection of Orf virus
... termed recombinase polymerase amplification(RPA), has been developed as an alternative to PCR ...a recombinase, a single-stranded DNA-binding protein (SSB) and a strand-displacing ...
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Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay
... The recombinase polymerase amplification (RPA) assay is an isothermal amplification system ...The amplification of the DNA in the RPA relies on enzymes and proteins to replace the ...
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Operational utility of the reverse transcription recombinase polymerase amplification for detection of dengue virus
... Background: A method for rapid detection of dengue virus using the reverse-transcription recombinase polymerase amplification (RT-RPA) was recently developed, evaluated and made ready for deployment. ...
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Rapid Detection of HIV 1 Proviral DNA for Early Infant Diagnosis Using Recombinase Polymerase Amplification
... dependent amplification (HDA) (40, 41), loop-mediated amplifi- cation (LAMP) (37, 41–43), nucleic acid sequence-based amplifi- cation (NASBA) (44, 45), and recombinase polymerase amplification ...
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Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis
... successful amplification or visual detection of Perkinsus beihaiensis, ...RPA, recombinase polymerase amplification; T, test line; VH, Vibrio harveyi ...
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Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via Recombinase Polymerase Amplification
... transcription-recombinase polymerase amplification (RT-RPA), magnetically-isolated, and detected through horseradish peroxidase (HRP)-catalyzed colorimetric ...
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Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C gattii in cerebral spinal fluid
... with recombinase polymerase amplification (LF-RPA) assay was constructed to detect the specific DNA sequences of ...and amplification temperature were also ...As amplification template ...
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Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood
... Culture based methods are the gold standard for diag- nosing invasive pneumococcal disease (IPD). However culture based methods typically require at least several hours to a day or more incubation before an organism is ...
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Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of Schistosoma haematobium
... [15]. Polymerase chain reaction (PCR) and quan- titative PCR (qPCR) methods, that target and amplify schistosome DNA from urine and stool samples, have been shown to be sensitive ...isothermal amplification ...
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Development of real time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus
... three hundred copies within 20 min. The sequence alignment shows these two methods would be also cap- able of detecting LSDV, which causes lumpy skin disease in cattle. However, their effectiveness for detecting LSDV in ...
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Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China
... called recombinase polymerase ampli- fication (RPA) for molecular diagnosis ...other amplification methods, RPA does not require thermal denaturation of template but utilizes recombinase ...
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Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis
... Plasmid DNA standard and real-time PCR. The tul4 gene was ampli- fied using the primers FTUL UP/DP (Table 2) and the genomic DNA of F. tularensis strain DuBa-1 using the following PCR protocol. The reaction mixture (50 ...
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