Recombinase polymerase amplification assay
Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood
... Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood Eoin Clancy1,2*, Owen Higgins1,2, Matthew S.. Forrest3, Tec[r] ...
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Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay
... RPA assay detected equivalent to one LD genomic DNA. The assay was performed at constant temperature (42 °C) in 15 ...RPA assay also detected other Leishmania species ...
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Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C gattii in cerebral spinal fluid
... LF-RPA assay from start of template pre- paration to detection on strips is about 35 ...LF-RPA assay presented here is a relatively ideal method for rapid and visual detec- tion of Cryptococcus ...
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Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China
... LFD-RPA assay which combine recombin- ase polymerase amplification and lateral flow dipstick was also ...LFD-RPA assay requires additional steps to transfer the amplified product to another ...
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Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids
... PKD-RPA assay was 10-times more sensi- tive than that of a diagnostic PCR developed by Kent et ...PCR assay developed by Fontes et ...PKD-RPA assay can be performed much faster (25 min) than the ...
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Field Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp capripneumoniae
... The assay detected the pathogen Mycoplasma capricolum ...diagnostic assay. We achieved assay specificity by engineering the RPA to reduce off-target binding and cross- reactivity with other closely ...
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Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis
... Plasmid DNA standard and real-time PCR. The tul4 gene was ampli- fied using the primers FTUL UP/DP (Table 2) and the genomic DNA of F. tularensis strain DuBa-1 using the following PCR protocol. The reaction mixture (50 ...
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Development of a fluorescent probe based recombinase polymerase amplification assay for rapid detection of Orf virus
... specific assay for detection and quantification of ORFV in clinical samples and is able to differentiate from related ...qPCR assay relies on specialized and expensive thermocycling machines, ...
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Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients
... Isothermal amplification techniques such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) for diagnosing Buruli ulcer, a necrotic skin ...
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Development of real time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus
... RPA assay was characterized with high sensitivity, good diagnostic specificity and rapidity, and represents a powerful po- tential to be a pen-side assay for rapid detection of ...The amplification ...
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Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection
... programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but requires infrastructure, financial resources and skilled personnel, often not available in endemic ...areas. ...
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Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis
... RPA assay that amplifies the ITS region of ...this assay to be more sensitive at detecting ...successful amplification or visual detection of Perkinsus beihaiensis, ...RPA, recombinase ...
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Rapid Detection of HIV 1 Proviral DNA for Early Infant Diagnosis Using Recombinase Polymerase Amplification
... efficacy. Recombinase polymerase amplification (RPA) is a novel technology that is ideal for an HIV-1 diagnostic, as it amplifies target DNA in <20 min at a constant temperature, without the need ...
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Early Detection of Dengue Virus by Use of Reverse Transcription Recombinase Polymerase Amplification
... RT-RPA assay. A DENV-specific TwistAmp RT exo lyophilized kit was supplied as strips of eight reactions in vacuum-sealed pouches (TwistDx, Ltd., Cambridge, United Kingdom). The RT-RPA was per- formed in a final ...
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Operational utility of the reverse transcription recombinase polymerase amplification for detection of dengue virus
... acid amplification test (NAAT) [1–4] or detection of dengue virus (DENV) nonstructural protein 1 (NS1) antigen especially that configured into the rapid de- tection test (RDT) format ...NS1 assay ...
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Development of real time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus
... number = (ng × 6.02 × 10 23 × 10 −9 )/ (Fragment length (bp) × 660). The DNA standard was then aliquoted and stored at −80 °C until used. To prepare CaPV- spiked tissue lysates, CaPV-free tissue samples of liver, lung, ...
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A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses
... LF-RPA assay demonstrated a higher sensitivity than both qPCR (2-fold increased sensitivity; ...PCR assay, which was also ~ 5-fold higher than that of LF-RPA developed in the present ...
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Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of Schistosoma haematobium
... RPA assay was successfully developed to amplify the Dra1 DNA repeat region of ...diagnostic assay, that has a detection limit of 10 fg of ...Dra1 assay (LF-ShDra1-RPA) [31], that had a detec- tion ...
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Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis
... as polymerase chain reaction (PCR), which amplify a specific nucleic acid sequence, have been developed over the past ...the assay and the still not-far-enough advanced port- able devices for point-of-care ...
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Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
... There is a real need in diagnostic laboratories for tech- nology that provides rapid and affordable diagnosis of vi- ruses and has the flexibility for downstream applications or applied disease management. This is ...
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