REP-PCR
Differentiation of Enterotoxigenic and Enteropathogenic Escherichia coli Isolated from Humans and Calves by rep-PCR
... This study was conducted to achieve a good perception of the genetic relationships and evolutionary patterns within E. coli pathotypes isolated from humans and calves. The rep-PCR DNA fingerprint, which ...
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Validation of use of whole cell repetitive extragenic palindromic sequence based PCR (REP PCR) for typing strains belonging to the Acinetobacter calcoaceticus Acinetobacter baumannii complex and application of the method to the investigation of a hospital outbreak
... sequence-based PCR (REP-PCR) typing protocol for members of the Acinetobacter ...same REP-PCR typing pattern as isolates of ...same REP-PCR pattern was isolated from it 10 ...
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Rep-PCR-based typing as a tool for tracking of MRSA infection origin
... the rep-PCR results, the identical quantity of template DNA (200 ng DNA) was added to each rep-PCR ...the rep-PCR analysis. The PCR composition was as follows: 1 μm ...
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Genetic diversity of Fusarium semitectum isolates from rice, using RAPD and REP-PCR markers
... observed that both the methods were convenient to determine the intra-species genetic variation in the isolates of the same species. The work carried out by Leslie et al. (2007) supports the findings of the present ...
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Evaluation of a High Throughput Repetitive Sequence Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains
... Repetitive-sequence-based PCR (rep-PCR) is useful for generating DNA fingerprints of diverse bacterial and fungal ...species. Rep-PCR amplicon fingerprints represent genomic segments ...
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Molecular epidemiology of Ornithobacterium rhinotracheale
... distinct rep-PCR fingerprint profiles (pro- files A and B) that differed from each other by a single band ...the rep-PCR fingerprints of all the outbreak isolates from Minnesota farms were ...
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Comparison of an Automated Repetitive Sequence Based PCR Microbial Typing System with Pulsed Field Gel Electrophoresis for Molecular Typing of Vancomycin Resistant Enterococcus faecium
... for Rep-PCR ...different Rep-PCR types (Table ...three Rep-PCR types (types a, h, and ...to Rep-PCR type a and type ...and Rep-PCR (P ⬍ ...comparing ...
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Whole genome sequencing for the molecular characterization of carbapenem resistant Klebsiella pneumoniae strains isolated at the Italian ASST Fatebenefratelli Sacco Hospital, 2012–2014
... In healthcare settings, Klebsiella bacteria can spread as a result of person-to-person contact or, less fre- quently, environmental contamination. Unfortunately, the retrospective nature of this study means that it has ...
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Evaluation of PCR Based Methods for Discrimination ofFrancisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
... after PCR-based amplification that are more reproducible than those seen after amplification with shorter random primers (6, ...7). PCR amplification based on different combinations of six such random ...
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Efficacy of Pulsed Field Gel Electrophoresis and Repetitive Element Sequence Based PCR in Typing of Salmonella Isolates from Assam, India
... In the PFGE analysis, the banding patterns revealed a total of 30 distinct polymor- phic bands, which ranged from 22 to 1,135 bp in size (Fig. 1). The bands of 22 bp and 249 bp were shared by all the isolates, while ...
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Genomic fingerprinting of Bartonella species by repetitive element PCR for distinguishing species and isolates
... by REP-PCR and by ...from PCR products from whole-cell or- ganisms harvested at different ...the REP-PCR product of these isolates with seven different restriction enzymes, while ...
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Microbial DNA Typing by Automated Repetitive Sequence Based PCR
... Finally, rep-PCR-based molecular typing is considered to have favorable costs (par- ticularly startup and equipment costs) associated with this platform, compared to other methods (2, 10, 13, 27, ...tomated ...
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Phenotypic and Genotypic Identification of NSLAB from Raw Cow Milk
... to rep-PCR as described Gevers et ...The PCR products were loaded on a 2 % agarose gel, run at a constant voltage of 80 V for 110 min and stained with ethidium bromide in 1×TBE buffer for 30 min, ...
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Discrimination of Escherichia coli isolates recovered from mucosal contents of chicken intestines and different age by repetitive elements sequence-based PCR
... of rep-PCR and differentiation of isolates based on dendrograms or fingerprints which are not as accurate as using discriminatory power based on Simpson’s index of diversity statistical analysis ...
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Use of PCR Based Mycobacterium tuberculosis Genotyping To Prioritize Tuberculosis Outbreak Control Activities
... the PCR mixtures and cycling conditions described by Supply et ...respectively. Rep-PCR with the Bacterial Barcodes (Athens, GA) mycobacterial kit was performed with genomic DNA concentrations of 25 ...
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Amplified Fragment Length Polymorphism Analysis: the State of an Art
... quence-based PCR (rep-PCR), and protein profiling ...and rep-PCR, but unlike am- plified ribosomal DNA restriction analysis (ARDRA), AFLPs correspond to mutations that are dispersed ...
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Repetitive Sequence Based PCR Using the DiversiLab System for Identification of Aspergillus Species
... on rep-PCR clustering in the following situations: (i) the identi- fication differed from conventional testing, (ii) the fingerprint pattern of randomly selected isolates did not match that of any other ...
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Strategy to detect and identify Bartonella species in routine clinical laboratory yields Bartonella henselae from human immunodeficiency virus positive patient and unique Bartonella strain from his cat
... identification, REP-PCR, RapID ANA, and CFA analysis, for 18 strains of Bartonella ...henselae. REP-PCR was the most useful test, because we found that it distinguished all known species of ...
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Comparative Evaluation of an Automated Repetitive Sequence Based PCR Instrument versus Pulsed Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak
... the rep-PCR DiversiLab Microbial Typing System (bioMe ´rieux, Italy), which amplifies the regions be- tween the noncoding repetitive sequences in bacterial genomes ...
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Repetitive Sequence PCR Based DNA Fingerprinting Using the DiversiLab System for Identification of Commonly Encountered Dermatophytes
... performing rep-PCR were blinded to the results of con- ventional testing (except in the initial part of the feasibility phase as described ...the rep-PCR master mix with fungal specific prim- ...
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