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Repetitive sequence-based PCR

DNA fingerprinting of Candida rugosa via repetitive sequence based PCR

DNA fingerprinting of Candida rugosa via repetitive sequence based PCR

... A repetitive sequence-based PCR (rep-PCR) technique was developed to characterize the genotypic relat- edness among Candida rugosa ...Two repetitive sequences, ...of ...

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Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive Sequence Based PCR for Characterization of Staphylococcus aureus Strains

Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive Sequence Based PCR for Characterization of Staphylococcus aureus Strains

... The DiversiLab System, which includes microfluidics-based detection, reagent kits, and software for data processing and analysis, is an automated method using repetitive sequence-based ...

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Repetitive Sequence Based PCR Using the DiversiLab System for Identification of Aspergillus Species

Repetitive Sequence Based PCR Using the DiversiLab System for Identification of Aspergillus Species

... weeks, based on growth rate, colony morphology, and microscopic ...uses repetitive-sequence-based PCR (rep-PCR) to discriminate among bacterial and fungal isolates, accurately ...

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Evaluation of a High Throughput Repetitive Sequence Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains

Evaluation of a High Throughput Repetitive Sequence Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains

... Repetitive-sequence-based PCR (rep-PCR) is useful for generating DNA fingerprints of diverse bacterial and fungal ...between repetitive se- ...

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Comparative Evaluation of an Automated Repetitive Sequence Based PCR Instrument versus Pulsed Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak

Comparative Evaluation of an Automated Repetitive Sequence Based PCR Instrument versus Pulsed Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak

... semiautomated, repetitive-sequence-based PCR (rep-PCR) instrument (DiversiLab system) was evaluated in comparison with pulsed-field gel electrophoresis (PFGE) to investigate an outbreak ...

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Repetitive Sequence Based PCR versus Pulsed Field Gel Electrophoresis for Typing of Enterococcus  faecalis at the Subspecies Level

Repetitive Sequence Based PCR versus Pulsed Field Gel Electrophoresis for Typing of Enterococcus faecalis at the Subspecies Level

... Repetitive sequence-based PCR was compared to pulsed-field gel electrophoresis (PFGE) for the ability to discriminate Enterococcus faecalis isolates at the subspecies ...from repetitive ...

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Identification of Histoplasma capsulatum, Blastomyces dermatitidis, and Coccidioides Species by Repetitive Sequence Based PCR

Identification of Histoplasma capsulatum, Blastomyces dermatitidis, and Coccidioides Species by Repetitive Sequence Based PCR

... automated repetitive-sequence-based PCR (rep-PCR) using the Diversi- Lab system (Bacterial Barcodes, ...rep- PCR method uses primers that target and bind to multiple noncoding, ...

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Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive Sequence Based PCR

Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive Sequence Based PCR

... available repetitive-sequence-based PCR (rep-PCR) DNA fingerprinting assay adapted to an automated format, the DiversiLab system, enables rapid microbial identification and strain ...

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Microbial DNA Typing by Automated Repetitive Sequence Based PCR

Microbial DNA Typing by Automated Repetitive Sequence Based PCR

... Reproducibility of a genotyping method is critical for longi- tudinal epidemiological studies and for comparing archived fingerprint patterns. The data presented here (Fig. 2B) and in other studies (18, 45) indicate that ...

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Comparison of Five Repetitive Sequence Based PCR Typing Methods for Molecular Discrimination of Salmonella
enterica Isolates

Comparison of Five Repetitive Sequence Based PCR Typing Methods for Molecular Discrimination of Salmonella enterica Isolates

... within a serotype seems to be limited to the absence or pres- ence of mostly one and sometimes two bands for a primer or primer set or to differences in intensities of some bands. In a few restricted cases (e.g., MB1221 ...

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Discrimination of Escherichia coli isolates recovered from mucosal contents of chicken intestines and different age by repetitive elements sequence-based PCR

Discrimination of Escherichia coli isolates recovered from mucosal contents of chicken intestines and different age by repetitive elements sequence-based PCR

... rRNA PCR are only able to discriminate bacterial isolates up to species level [9, ...contrary, repetitive sequence-based PCR or rep-PCR is easy to perform, rapid and cheaper ...

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Suitability of Repetitive DNA Sequence Based PCR Fingerprinting for Characterizing Epidemic Isolates of Salmonella enterica Serovar Saintpaul

Suitability of Repetitive DNA Sequence Based PCR Fingerprinting for Characterizing Epidemic Isolates of Salmonella enterica Serovar Saintpaul

... methods based on the molecular analysis of the genome have become important for the epidemiological characterization of patho- ...primed PCR (AP-PCR) fin- gerprinting, and, lately, ...

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Repetitive Sequence PCR Based DNA Fingerprinting Using the DiversiLab System for Identification of Commonly Encountered Dermatophytes

Repetitive Sequence PCR Based DNA Fingerprinting Using the DiversiLab System for Identification of Commonly Encountered Dermatophytes

... of repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system for identification of dermatophytes commonly isolated in a clinical laboratory was assessed by comparing results ...

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Molecular epidemiology of Ornithobacterium rhinotracheale

Molecular epidemiology of Ornithobacterium rhinotracheale

... (MLEE), repetitive sequence based-PCR (rep-PCR), and 16S rRNA gene ...gene sequence and rep-PCR analyses confirmed the results obtained by MLEE and indicated limited ...

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Degenerate codon mixing for PCR based manipulation of highly repetitive sequences

Degenerate codon mixing for PCR based manipulation of highly repetitive sequences

... for PCR-based amplification of repetitive regions and aims to minimise the generation of non-specific PCR products and flawed repeats by exploiting the codon redundancy of the genetic code to ...

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Comparison of Different PCR Approaches for Typing of Francisella tularensis Strains

Comparison of Different PCR Approaches for Typing of Francisella tularensis Strains

... species. Repetitive element sequence-based PCR (rep-PCR) is a group of methods which generate DNA fingerprints that allow discrimination between bacterial ...of repetitive ...

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PCR Based Methods for Genotyping Viridans Group Streptococci

PCR Based Methods for Genotyping Viridans Group Streptococci

... any PCR products, so these primers were not tested further with any of the other ...is based on the sequence of a repetitive element identified in Streptococcus pneumoniae ...

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Molecular genotyping of methicillin resistant Staphylococcus aureus via fluorophore enhanced repetitive sequence PCR

Molecular genotyping of methicillin resistant Staphylococcus aureus via fluorophore enhanced repetitive sequence PCR

... exploits PCR am- plification from a DNA repeat sequence in MRSA is ...repeat sequence provides an ideal target for detecting DNA fragment patterns specific to individual MRSA ...Two PCR ...

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Genotypic diversity among multidrug resistant Pseudomonas aeruginosa and Acinetobacter species at Mulago Hospital in Kampala, Uganda

Genotypic diversity among multidrug resistant Pseudomonas aeruginosa and Acinetobacter species at Mulago Hospital in Kampala, Uganda

... the repetitive sequence elements on which Rep-PCR genotyping is based are highly conserved throughout the prokaryotic kingdom [30] and have been used extensively in the surveillance of ...

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