Reporter Gene Assay
Enhanced reporter gene assay for the detection of osteogenic differentiation
... monitoring. Reporter gene assays employing tissue-specific promoters and suitable reporter genes fulfill these ...sensitive reporter gene ...a reporter gene assay ...
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Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay
... was observed at the ER binding assay. Interestingly, the weak estrogenic activity was also observed at high concen- tration (0.1 mM) of PES (in the dose response assay, the highest concentration of PES was ...
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Novel Replicon Based Reporter Gene Assay for Detection of Rubella Virus in Clinical Specimens
... Transfection and reporter gene expression. The replicon constructs RUBrep/ GFP, RUBrep/GFP-⌬NotI, RUBrep/CAT, and RUBrep/CAT-⌬NotI constructs were described previously (18, 19). The plasmids were purified ...
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PA Residues in the 2009 H1N1 Pandemic Influenza Virus Enhance Avian Influenza Virus Polymerase Activity in Mammalian Cells
... The 2009 pandemic influenza virus (pH1N1) is a swine-origin reassortant containing human, avian, and swine influenza genes. We have previously shown that the polymerase complex of the pH1N1 strain A/Cali- fornia/04/2009 ...
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Androgen receptor antagonism drives cytochrome P450 17A1 inhibitor efficacy in prostate cancer
... transcriptional activity in a cell-based reporter gene assay (Table 1). On the AR-T877A mutation, hydroxyflutamide activated, while enzalutamide and bicalutamide inhibited, transcription. Impor- ...
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Minor change, major difference: divergent functions of highly conserved cis regulatory elements subsequent to whole genome duplication events
... ancestral gene function was divided between ...PAX2/5/8 gene subfamily appears to support at least the first part of this ...PAX2/5/8 gene subfamily, PAX2 is associated with the highest number of ...
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A heptanucleotide sequence mediates ribosomal frameshifting in mammalian cells.
... The difference in frameshifting efficiency between our reporter gene assay system and the native HIV-1 sequence in vivo and in vitro may be explained by technical differences or by biolo[r] ...
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Identification of Hendra Virus G Glycoprotein Residues That Are Critical for Receptor Binding
... The reporter gene assay was conducted as described earlier for each mutant or WT effector cell population, and then the raw -Gal readings were normalized to compensate for the difference between ...
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Deleterious effects of estrogenic endocrine disruptors on marine organisms: Histological Observed Effects and Some Novel Useful Monitoring Bioassays
... To assess the extent of estrogenic disruptor chemicals in the marine environment we used an “in vitro” short term test such as the recombinant yeast reporter gene assay. Both in situ and in vivo ...
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Inhibition of E2 induced expression of BRCA1 by persistent organochlorines
... the reporter gene assay are also valid for genomic BRCA1 mRNA expression, northern blot analysis was carried out to measure the effect on the BRCA1 mRNA level with each compound at 10 µ M, except for ...
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Polymorphisms in the SULF1 gene are associated with early age of onset and survival of ovarian cancer
... luciferase reporter gene assay of rs2623047 G>A further showed that the G allele exhib- ited slightly higher promoter activity in SKOV-3 and HeLa cancer cell lines, which is consistent with one ...
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A46R and A52R from vaccinia virus are antagonists of host IL 1 and toll like receptor signaling
... NFB reporter gene activity was then ...NFB reporter gene activity was then ...a reporter gene ...a reporter gene ...
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LINC00152 promotes proliferation in hepatocellular carcinoma by targeting EpCAM via the mTOR signaling pathway
... (NCBI) Gene data- base (http://www.ncbi.nlm.nih.gov/gene) and found an mTOR-related gene, EpCAM, located near LINC00152 (Figure ...luciferase reporter gene assay showed that the ...
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Liesner, Raphael (2010): Mutational analysis of PhiC31 integrase to improve gene therapeutic applications. Dissertation, LMU München: Fakultät für Biologie
... analysed for the genetic content near the crossover site. The databases of bioinformatics websites were browsed for ORFs at or in the proximity of particular insertion sites. The human BLAST database or nucleotide BLAST ...
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Cloning and regulation of the rat bradykinin B1 receptor gene
... At the onset of these studies, the in vivo and in vitro characterisation of the BKBi receptors had been performed using pharmacological agents with no molecular confirmation of the up-regulation process. Moreover, ...
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Positron Emission Tomography Reporter Genes and Reporter Probes: Gene and Cell Therapy Applications
... Herpes simplex virus 1 thymidine kinase (HSV1-tk) and its mutant derivatives have thus far been the most extensively studied and applied PET IRGs. These PET IRGs encode enzymes that catalyze phosphorylation reactions ...
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A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
... AGT gene expression [2, 9], and the rs7079 polymorph- ism may affect AGT gene function, [19] it is possible that rs7079 may play a role in lead ...AGT gene would be associated with lead ...
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Original Article MiR-455-DNMT3A signaling is linked toacquisition of doxorubicinresistance in breast cancer
... luciferase reporter assay, MTT and gene silencing to investigate the role of miR-455 in the resistance of human MCF-7 breast adenocarcinoma cells to doxorubicin ...
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Developing tTA Transgenic Rats for Inducible and Reversible Gene Expression
... the gene is con- stitutively ...one gene of the JNK family causes com- pensatory changes in the other related genes ...the gene dis- rupted from germline suggest a negative regulatory role in cell ...
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Transcriptome wide effects of inverted SINEs on gene expression and their impact on RNA polymerase II activity
... affects gene expression or is only tolerated in genes with low ...reduce gene expression, our data, for the first time, show a transcriptome-wide reduction of iSINE-containing ...
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