Restriction endonuclease analysis (REA)
Restriction endonuclease analysis of nosocomial isolates of Clostridium difficile
... A total of 110 clinical isolates of Clostridium difficile were analyzed by agarose gel electrophoresis by using both bacterial restriction endonuclease analysis (REA) and plasmid profile[r] ...
5
Identification of Nocardia Species by Restriction Endonuclease Analysis of an Amplified Portion of the 16S rRNA Gene
... subsequent restriction endonuclease analysis (REA) for species ...Unique restriction fragment length polymorphism (RFLP) patterns were found for Nocardia ...sequence analysis suggested ...
7
Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species
... In contrast, bacterial chromosomal restriction endonuclease analysis in conjunction with Southern hybridizations was most effectively used to determine the significance of low levels of [r] ...
5
Molecular epidemiology of Shigella infections: plasmid profiles, serotype correlation, and restriction endonuclease analysis
... When this plasmid was used as a probe, strong homology was seen with numerous small plasmids in all Shigella species; restriction endonuclease analysis revealed a 1.1-kb A val-A vall.. f[r] ...
5
Typing of Chlamydia trachomatis by restriction endonuclease analysis of the amplified major outer membrane protein gene
... A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP)..[r] ...
5
Comparison of avian Chlamydia psittaci isolates by restriction endonuclease analysis and serovar specific monoclonal antibodies
... Avian Chlamydia psittaci isolates were examined by restriction endonuclease analysis and serovar-specific monoclonal antibodies and compared with ovine abortion and polyarthritis isolate[r] ...
6
Restriction Endonuclease Analysis Discriminates Bordetella bronchiseptica Isolates
... Received 10 April 2000/Returned for modification 16 August 2000/Accepted 21 September 2000 One hundred ninety-five Bordetella bronchiseptica isolates from 12 different host species worldwide were characterized by ...
7
Typing of Aeromonas strains by DNA restriction endonuclease analysis and polyacrylamide gel electrophoresis of cell envelopes
... Twenty-three strains from primary and repeat fecal cultures of samples from 11 patients with Aeromonas-related diarrhea were examined both by OMP and REA typing: 2 patients had isolates [r] ...
6
Correlation between restriction endonuclease analysis and PCR ribotyping for the identification of Clostridioides (Clostridium) difficile clinical strains
... The relationship between REA types and PCR ribotypes was more complex in this study compared to the previous formal comparison of these 2 typing systems [11]. Previously, a particular REA group typically correlated with ...
8
Restriction endonuclease analysis of Eikenella corrodens
... Group 1 included 47 epidemiologically independent isolates recovered from dental plaques in periodontally healthy subjects and periodontitis patients and from extraoral infections in dif[r] ...
6
Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis
... As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA[r] ...
6
Restriction endonuclease analysis of DNA from Chlamydia trachomatis biovars
... isolates generated similar fragment patterns; however, distinct fragments appeared to be unique to both biovars, thus allowing differentiation of these two major groups.. In most cases, [r] ...
5
Restriction Endonuclease Analysis of Cytomegalovirus Deoxyribonucleic Acid as an Epidemiologic Tool
... Culture Recognized In Situ Cytohybridi- zation Index case Pregnant physician Fetus 7/14/76 7/14/76 9/1/76 9/17/76 9/17/76 9/23/76 10/18/76 10/19/76 10/19/76 Urine CSF Cervical swab Throa[r] ...
7
Examination of uncommon clinical isolates of human adenoviruses by restriction endonuclease analysis
... Restriction endonuclease digest patterns of DNA of a reference strain of adenovirus 41 (subgenus G) with two clinical isolates (6511 and 4717), which showed an identical pattern with Kpn[r] ...
6
Identification of genomic clonal types of Actinobacillus actinomycetemcomitans by restriction endonuclease analysis
... (Fig. lb) manifested two more distinct DNA fragment pat- terns. lb, lane 1) was designated Sall type VII. lb, lanes 2 to 4) produced identical DNA fragment patterns with Sall and were de[r] ...
5
Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis
... Fifty Campylobacterjejuni isolates, including 29 from humans associated with an outbreak of enteritis, 20 from cattle, and 1 from a milk source, were serotyped on the basis of extractabl[r] ...
5
PCR and Restriction Endonuclease Analysis for Rapid Identification of Human Adenovirus Subgenera
... different restriction fragments, one enzyme, BseRI, differentiates between Ad8 and Ad10 because it cuts the latter but does not cut the ...RE analysis with MnlI. Unfortunately, in the case of RE ...
7
Restriction endonuclease analysis of Staphylococcus epidermidis DNA may be a useful epidemiological marker
... Strains 4, 5, 10, 14, 12, and 13 isolated from patient H may derive from a single strain, since the antibiotic resistance characteristics and plasmid profiles were closely related. The 2[r] ...
6
Restriction endonuclease analysis of human and bovine group B streptococci for epidemiologic study
... care unit outbreak isolate, type lb/c (no.. Double digestion of GBS isolates with BgIII and then EcoRI. 437); 2 to 4, type lb/c neonatal intensive care unit outbreak isolates (no. 4 and [r] ...
5
Restriction endonuclease analysis and ribotyping differentiate genital and nongenital strains of Bacteroides ureolyticus
... ureolyticus reference strains NCTC 10940, NCTC 10941, NCTC 10948, and NCTC 10949 were positive in the urease, oxidase, and nitrate tests, while strain NCTC 10939 was positive in the nitr[r] ...
7