reticulocyte lysate
Alphacoronavirus Transmissible Gastroenteritis Virus nsp1 Protein Suppresses Protein Translation in Mammalian Cells and in Cell-Free HeLa Cell Extracts but Not in Rabbit Reticulocyte Lysate
... rabbit reticulocyte lysate (RRL; Promega) for 10 min (A) or HeLa S10 extract for 30 min (B) in the presence of 1 g of purified GST, TGEV nsp1 (TGEV), SCoV nsp1 (SCoV), or SCoV nsp1-mt (SCoVmt), and rLuc ...
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La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate.
... These studies stem from the initial observation that translation of poliovirus RNA in a rabbit reticulocyte lysate is very inefficient and produces aberrant polypeptides, but translation[r] ...
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Removal of the genome-linked protein of foot-and-mouth disease virus by rabbit reticulocyte lysate.
... Although it has been shown that the migration of S fragment of FMDV RNA is affected by treatment of the RNA with a rabbit reticulocyte lysate, it is not clear whether this treatment brea[r] ...
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In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate.
... The absence of these P3 region proteins from the poliovirus-specific translation product in HeLa cell extract or the proteins found in infected cells suggests that this internal initiati[r] ...
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Evidence that vesicular stomatitis virus produces double-stranded RNA that inhibits protein synthesis in a reticulocyte lysate.
... Effect of melting and micrococcal nuclease on the residual fractional capacity of polyA+ RNA from VSV-infected HeLa cells and polyI:polyC to inhibit protein synthesis by reticulocyte lys[r] ...
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Cleavage of a viral polyprotein by a cellular proteolytic activity.
... The 200K polyprotein was synthesized in the reticulocyte lysate in the absence of DTT, and then the reaction mixtures were serially diluted either with a blank lysate translation solutio[r] ...
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Maturation of giardiavirus capsid protein involves posttranslational proteolytic processing by a cysteine protease.
... signal proteases before myristoylation and glycosylation of the viral proteins can occur (12). In the case of Sindbis virus, the viral polymerase must be degraded through the cellular ubiq- uitin pathway to stop the ...
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Elevated adenosine deaminase activity and hereditary hemolytic anemia Evidence for abnormal translational control of protein synthesis
... Translation of proband poly A+ reticulocyte mRNA in a rabbit reticulocyte lysate system and immunoprecipitation of 35S-labeled protein products with anti-ADA antibody yielded a band of a[r] ...
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Cell type-specific proteins which interact with the 5' nontranslated region of hepatitis A virus RNA.
... Label transfer to ribosomal salt wash RSW proteins prepared from HeLa cells A and rabbit reticulocyte lysate RRL B after UV cross-linking to RNA probes representing different segments of[r] ...
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The E1∧E4 Protein of Human Papillomavirus Type 16 Associates with a Putative RNA Helicase through Sequences in Its C Terminus
... The region of E4-DBP involved in the association with E1∧E4 in yeast was expressed from pET28a using a rabbit reticulocyte lysate and shown to bind MBP.16 E1∧E4 immobilized on amylose re[r] ...
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Synthesis of Ribonucleic Acid in Rabbit Blood Cells In Vivo
... RNA was isolated from 1 the total population of washed cells, which includes reticulocytes and leukocytes; 2 shock lysates, which represent primarily reticulocyte lysate; and 3 the sedim[r] ...
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Cell-free translation of murine coronavirus RNA.
... The six murine hepatitis virus-specific subgenomic RNAs were partially purified by agarose gel electrophoresis and translated in an mRNA-dependent rabbit reticulocyte lysate, and the cel[r] ...
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A translation strategy and gene map for pea seedborne mosaic virus
... Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins of tobacco etch virus RNA translation and cylindrical inclusion protein as a pr[r] ...
153
Synthesis of Vesicular Stomatitis Virus Negative-Strand RNA In Vitro: Dependence on Viral Protein Synthesis
... In these experiments the amount of negative-strand RNA synthesized in the complete system consisting of nucleocapsid templates and reticulocyte lysate programmed with saturating amounts [r] ...
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Expression of Bottom Component RNA of Cowpea Mosaic Virus in Cowpea Protoplasts
... Comparison of the peptide patterns of the virusinduced proteins with those of the cowpea mosaic virus RNA-coded polypeptides produced in rabbit reticulocyte lysate showed that the 170- a[r] ...
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A heat-sensitive inhibitor in poliovirus-infected cells which selectively blocks phosphorylation of the alpha subunit of eucaryotic initiation factor 2 by the double-stranded RNA-activated protein kinase.
... dsRNA-induced inhibition of in vitro translation in reticulocyte lysate could be prevented byi the addition of partially purified inhibitor during preincubation of the Iysate with dsRNA,[r] ...
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Rous Sarcoma Virus Gag Has No Specific Requirement for Phosphatidylinositol-(4,5)-Bisphosphate for Plasma Membrane Association In Vivo or for Liposome Interaction In Vitro
... commercial reticulocyte lysate, and the crude mixture was incubated with extruded, 100-nm PC/PS (2:1) liposomes with or without added ...the reticulocyte reaction was analyzed in the absence of added ...
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Identification of adenovirus 2 early region 4 polypeptides by in vitro translation and tryptic peptide map analysis.
... The E4-specific mRNA was also translated in a wheat germ lysate to identify polypeptides that may comigrate with the globin synthesized in the reticulocyte lysate, and to attempt to iden[r] ...
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Immunization of mice with soluble lysate of interferon gamma expressing Plasmodium berghei ANKA induces high IFN-γ production
... Soluble lysate of transgenic PbA (TPbA) was prepared and concentration of IFN- γ in lysate determined by ...with lysate of TPbA containing 75 pg/ml IFN- γ and live TPbA parasites ...with ...
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Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia
... reticulocyte lysates of patients with homozygous beta thalassemia and sickle cell anemia. The messenger RNA stimulated the synthesis of human globin chains by a cell-free system derived from Krebs mouse ascites ...
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