Retinal Pigment Epithelial Cell
Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture
... Low-level, steady electric fields of 6-10 voltskm stimulated directional orientation and translocation of cultured human retinal pigment epithelial cells.. The orientative movements galv[r] ...
8
Dysregulated claudin 5 cycling in the inner retina causes retinal pigment epithelial cell atrophy
... and retinal endothelial tight junctions ...in retinal claudin-5 expression was observed in the evening compared with the morning at both the protein and transcript level (Figure 1D, P = ...
14
Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
... It has been demonstrated that physiological stretch has a protective effect on cells [25], whereas pathological stretch may induce cell apoptosis [26]. Based on Annexin-V/PI double staining, we found that the ...
9
Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion
... and cell proliferation are associated with PVR development, how- ever, the mechanism underlying the relationship between ECM deposition and cell proliferation and PVR devel- opment is ...inflammation, ...
9
Influence of Cell Confluency on the Expression of the α4 Integrin Subunit of Retinal Pigment Epithelial Cells
... mediate cell-cell and cell-extracellular matrix ...at cell-cell junctions in post-confluent RPE cell cultures negatively correlated with the level of expression of the mRNA ...
10
Effect of curcumin on aging retinal pigment epithelial cells
... RPE cell line (ARPE19) was sourced from the American Type Culture Collection (Manassas, VA, ...The cell culture was done at 37 ° C in a humidified incubator with 5% CO 2 ...
8
Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
... The presence of the apoptotic marker cytochrome C was evaluated using western blot. RGC-5 and ARPE-19 cells were counted and processed for protein isolation according to the manufacturer’s instructions (Ambion). In ...
7
Osmolarity and spectrophotometric property of brilliant blue green define the degree of toxicity on retinal pigment epithelial cells exposed to surgical endoilluminator
... X-100. Cell permeabilization was quenched using 30% H 2 O 2 followed by addition of blocking buffer for 30 ...and cell number was calculated, normalized, and expressed in ...
9
Original Article Plasticity of adult human retinal pigment epithelial cells
... stem cell transdifferenti- ation to neural cells, by stimulating the expres- sion of synapsin I, a mature neuron marker, dur- ing the formation of cholinergic and dopaminer- gic neurons ...RPE cell ...
15
Original Article Effect of bradykinin on cultured retinal pigment epithelial cells
... The human retinal pigment epithelia cell line ARPE-19 was donated by Professor Guotong Xu from the Tongji Eye Institute. ARPE-19 cells were cultured in RPE medium (Dulbecco’s mod- ified Eagle’s ...
9
HIV 1 Tat protein alter the tight junction integrity and function of retinal pigment epithelium: an in vitro study
... Methods: A human RPE cell line (D407) cultured on microporous filter-supports was used. After treating with HIV-1 Tat protein, transepithelial electrical resistance (TER) of confluent RPE cells was measured by ...
11
Ion channels of cultured human retinal pigment epithelial cells
... RPE cells in culture are initially undifferentiated and seem to require a precisely defined environment to redifferentiate into cells which resemble those in vivo and display some degree of polarity. Several components ...
228
Ocular stem cells: a status update!
... organ. Cell-based therapies using stem cells from the ciliary body, iris and sclera are still in animal experimental stages but show potential for replacing degenerated ...Iris pigment epithelial, ...
12
Optimization of an in vitro bilayer model for studying the functional interplay between human primary retinal pigment epithelial and choroidal endothelial cells isolated from donor eyes
... of cell specific markers such as vWF as an endothelial specific marker for hCEC and cytokera- tin 18 as an epithelial specific marker for hRPE ...of retinal epithelial cells is essential for ...
7
Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
... Notes: (A) Exposure of cells (passage 4, 12 weeks in low-serum medium) to 0.25 and 0.5 mg/mL AV-17 for 5 minutes compromised the integrity of the adherens junctions, as detected by the increase in the cytoplasmic and ...
11
Docosahexaenoic acid (DHA) in stroke, Alzheimer’s disease, and blinding retinal degenerations: coping with neuroinflammation and sustaining cell survival
... stressed retinal pigment epithelial [RPE] cells, and in human brain cells exposed to amyloid-b ...when cell homeo- stasis is threatened by injury or neuro- ...
6
Deletion of TSPO resulted in change of metabolomic profile in retinal pigment epithelial cells
... induces cell apoptosis that is known to be the initial process of AMD disease ...of cell membranes, which is enriched in neural ...neural cell proliferation, differentiation, and apoptosis. RPE ...
17
Wogonin modulates hydroperoxide-induced apoptosis via PI3K/Akt pathway in retinal pigment epithelium cells
... RPE cell survival and thus may protect RPE cells from oxidant-induced cell death under normal circumstances and in abnormal states such as AMD ...peroxide-induced cell death was promoted by ...
6
Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage
... The expression of RPE specific genes was further analysed with quantitative RT-PCR by utilising TaqMan ® Gene Expression Assays (Applied Biosystems Inc.) with FAM-labeled primers for retinal pigment ...
20
Retinal pigment epithelial cells in vitro: Behaviour and the cytoskeleton with particular emphasis on cytokeratins
... A feature that was identified in cultured bovine RPE was that these cells exhibited inconsistent immunoreactivity to our panel of anti-cytokeratin MABs. Bovine cells were often negative for cytokeratin IFs, only ...
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