retroviral vectors
Regulated Gene Expression in the Chicken Embryo by Using Replication-Competent Retroviral Vectors
... with retroviral vectors will be of value for introducing hetero- geneous genes into restricted regions such as the RPE and the liver, where conventional retroviral vectors do not function ...
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Instability of retroviral vectors with HIV-1-specific RT aptamers due to cryptic splice sites in the U6 promoter
... of retroviral vectors play a role in gene expression and ...aptamer vectors generated using a shuttle packag- ing ...same vectors between ampho- tropic- and GaLV-pseudotyped Phoenix packaging ...
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Retroviral vectors and transposons for stable gene therapy: advances, current challenges and perspectives
... MLV vectors have the biological disadvantage that they are unable to effi- ciently transduce non-dividing or slowly dividing ...MLV vectors were gradually replaced by len- tiviral vectors based on ...
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Packaging of Endogenous Retroviral Sequences in Retroviral Vectors Produced by Murine and Human Packaging Cells
... produce retroviral vectors less contaminated with ERV se- quences and therefore less likely to give rise to unwanted ERV mobilization and generation of RCR due to recombination between ERV and vector ...
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E- vectors: development of novel self-inactivating and self-activating retroviral vectors for safer gene therapy.
... self-activating retroviral vectors based on the previously observed high-frequency deletion of direct ...viral vectors that contained large direct repeats flanking the viral encapsidation sequence ...
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Manipulation of chick embryonic development using retroviral vectors
... defective retroviral vectors have been used to investigate the lineage of various cells during development, for example neural crest cells (Frank and Sanes, 1991) and the nervous system (Price et aU ...
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Psi- vectors: murine leukemia virus-based self-inactivating and self-activating retroviral vectors.
... self-activating vectors to show that the previously demonstrated high-frequency direct repeat deletions are not unique to spleen necrosis virus (SNV) or the neomycin drug resistance ...gene. Retroviral ...
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Split-Intron Retroviral Vectors: Enhanced Expression with Improved Safety
... expression vectors leads to a fraction of the resulting transcripts being ...the retroviral replication process to copy not only the U3 promoter but also a synthetic splice donor to the 5 ⴕ ...
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Antibody-Directed Targeting of Retroviral Vectors via Cell Surface Antigens
... targeting retroviral vectors to specific ...for retroviral transduction, the virus titers are relatively high and stable and can be further increased by ...
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Construction of Retroviral Vectors with Improved Safety, Gene Expression, and Versatility
... (MLV)-based retroviral vectors are the most frequently used gene delivery ...current vectors are still not fully optimized for gene expression and viral titer, and many genetic and biochemical ...
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Use of Intron-Disrupted Polyadenylation Sites To Enhance Expression and Safety of Retroviral Vectors
... of retroviral expression vector with improved ...a retroviral expression vector, such a signal, although reformed in the producer cell, remains benign until transduction, where it is then preferentially ...
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Infection by retroviral vectors outside of their host range in the presence of replication-defective adenovirus.
... of retroviral vectors for clinical gene therapy is concern about the possibility of infectious spread of replication-competent retrovirus arising by natural recombination between the defective ...
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Development of Improved Adenosine Deaminase Retroviral Vectors
... using retroviral vectors have illuminated limitations with current retroviral vector technol- ...constructing retroviral vectors without dominant selectable markers and by testing these ...
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Infection efficiency of T lymphocytes with amphotropic retroviral vectors is cell cycle dependent.
... To establish the role of the host cell cycle in determining the efficiency of infection with retroviral vectors, we generated cultures of TJF-2 cells enriched in either the Go-G1 phase o[r] ...
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Generation of Transduction-Competent Retroviral Vectors by Infection with a Single Hybrid Vaccinia Virus
... viral vectors that express retroviral vector components and give rise to transducing par- ticles have been ...expressing retroviral packaging functions that can mobilize vector ge- nomes from a ...
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Multiple modifications in cis elements of the long terminal repeat of retroviral vectors lead to increased expression and decreased DNA methylation in embryonic carcinoma cells.
... produce retroviral vectors which overcome the inherent inactivity of the MoMuLV transcrip- tional unit in embryonic ...duce vectors with an internal promoter to mediate gene tran- scription in ES ...
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HOXB4 and retroviral vectors: adding fuel to the fire
... The transcription factor homeobox B4 (HOXB4) is a promising agent capable of providing a growth advantage to genetically modified hematopoietic stem and progenitor cells (HSPCs). In this issue of the JCI, Zhang and ...
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Design of 5′ Untranslated Sequences in Retroviral Vectors Developed for Medical Use
... splice-defective vectors and vectors utilizing IRES sequences or internal promoters for translational control of the trans- ...The vectors presented in this study were based on FMEV (a Friend mink ...
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Comparison of expression in hemopoietic cells by retroviral vectors carrying two genes.
... To assess the utility of the different vectors bearing the Neor for expression of a second gene in hemopoietic cells, we first analyzed the levels of viral RNA in FDC-P1 cells infected w[r] ...
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Oncogenicity of human N-ras oncogene and proto-oncogene introduced into retroviral vectors.
... In order to study the in vitro and in vivo oncogenicity of N-ras and to compare its pathogenicity to that of H-ras, we have inserted an activated or a normal form of human N-ras cDNA int[r] ...
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