reverse transcription-PCR
Reverse transcription PCR detection of hepatitis G virus
... the reverse transcription-PCR (RT- PCR) assay with the Enzymun-Test DNA detection system (Boehringer Mannheim GmbH, Mannheim, Germany) and made this technique applicable for mass screening in ...
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Comparison of Commercial Real Time Reverse Transcription PCR Assays for Reliable, Early, and Rapid Detection of Heterologous Strains of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected or Noninfected Boars by Use of Different Sample Types
... time reverse transcription-PCR (RT-PCR) assays for detection of genetically diverse PRRSV isolates in serum, semen, blood swabs, and oral fluids collected from experimentally infected boars ...
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Multiplex Reverse Transcription PCR for Simultaneous Surveillance of Influenza A and B Viruses
... multiplex reverse transcription-PCR (RT- PCR) method that amplifies the most critical genomic segments (hemagglutinin [HA], neuraminidase [NA], and matrix [M]) of seasonal influenza A and B ...
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Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... (multiplex) reverse transcription-PCR (RT-PCR) for detection, typing, and subtyping of the hemagglutinin gene of influenza type A (H3N2 and H1N1) and type B viruses was developed and applied ...
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Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... TaqMan reverse transcription-PCR (RT-PCR) assay specific for the detection of influenza A H5 viruses from clades 1, 1 ⴕ , 2, and 3 is ...
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Preclinical Evaluation of Two Real Time, Reverse Transcription PCR Assays for Detection of the Severe Acute Respiratory Syndrome Coronavirus
... We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR RT-PCR assay targeting the open reading frame ORF 1b region of the se[r] ...
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Standardized positive controls for detection of norovirus by reverse transcription PCR
... Background: Norovirus is one of the most common causes of nonbacterial gastroenteritis in humans. Rapid spread by contaminated food and person-to-person transmission through the fecal-oral route are characteristics of ...
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Performance and Cost Evaluation of One Commercial and Six In House Conventional and Real Time Reverse Transcription PCR Assays for Detection of Severe Acute Respiratory Syndrome Coronavirus
... Despite certain noteworthy characteristics of SARS, namely, the absence of upper respiratory tract symptoms, the presence of dry cough, and minimal auscultatory findings, with consoli- dation on chest radiographs, the ...
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Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... multiplex reverse transcription-PCR (RT-PCR) assay was developed to allow in one test the detection of nine different microorganisms (enterovirus, influenza A and B viruses, respiratory ...
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Quantitative Competitive Reverse Transcription PCR for Quantification of Dengue Virus RNA
... Using in vitro-transcribed RNA as a template, this assay can reliably detect 10 to 2,000 copies of dengue virus RNA per reaction. The sensitivity of the assay was estimated to be 10 to 50 copies per reaction. This assay ...
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Real Time Reverse Transcription PCR Assay Panel for Middle East Respiratory Syndrome Coronavirus
... Although the MERS-CoV rRT-PCR assay panel proved both sensitive and specific, the study was subject to several limitations. First, only two authentic specimens from patients with indepen- dently confirmed MRS-CoV ...
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Detection of Human Picornaviruses by Multiplex Reverse Transcription PCR and Liquid Hybridization
... multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was ...
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Enhanced Reverse Transcription PCR Assay for Detection of Norovirus Genogroup I
... of reverse primer NV193, 150 nM of probe NVGG1-LNA (6-carboxyfluorescein [FAM]–ATTCGGGCAGGAGAT– EclipseDQ [Eurogentec, Seraing, Belgium]; locked nucleic acid [LNA] nucleo- tides in underlined positions), and ...l ...
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Reverse Transcription PCR Detection ofMycobacterium leprae in Clinical Specimens
... Detection of RNA rather than DNA may be useful for the detection of viable organisms. The half-life of mRNA may be as little as 2 min following cell death. Several investigators have shown that mRNA detection is a ...
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Applications of Competitor RNA in Diagnostic Reverse Transcription PCR
... real-time PCR methodology, one additional potential advantage of heterolo- gous competitor RNA is that universal probes for use in real- time RT-PCR could be designed to detect the plasmid se- quence that ...
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Real time reverse transcription PCR based sequencing independent pathotyping of Eurasian avian influenza A viruses of subtype H7
... The PCR reactions were carried out on a CFX96 thermocycler machine (Bio-Rad) using the following temperature profile: 30 min at 50 °C (RT), 2 min at 94 °C (inactivation of reverse Table 1 Outbreaks in ...
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Determination of hepatitis C virus genotypes in the United States by cleavase fragment length polymorphism analysis
... We describe the application of a new DNA-scanning method, which has been termed Cleavase Fragment Length Polymorphism (CFLP; Third Wave Technologies, Inc., Madison, Wis.), for the determination of the genotype of ...
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Expression of Six Chloroplast Genes in Jatropha curcas Callus under Light and Dark Conditions
... the reverse transcription PCR, whereas the gene was not transcribed in the green callus under ...real-time PCR was carried out to detect the part of ycf 2 chloroplast gene induction under ...
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Quantitation of hepatitis C virus genome molecules in plasma samples
... A competitive reverse transcription PCR cRT-PCR-based assay for the quantitative detection of hepatitis C virus HCV viremia was developed, optimized, and applied to the direct molecular [r] ...
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New PCR Assay Using Glucose 6 Phosphate Dehydrogenase for Identification of Leishmania Species
... for reverse transcription-PCR experiments, designed from the nucleotide sequence of the PCR product, enabled us to characterize the 5 ⴕ and 3 ⴕ untranslated regions and the G6PD open reading ...
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