Reverse transcription (RT) – nested PCR
Duplex Reverse Transcription PCR Followed by Nested PCR Assays for Detection and Identification of Brazilian Alphaviruses and Flaviviruses
... duplex reverse transcription-PCR (D-RT-PCR), followed by multiplex nested PCR (M-N-PCR) or nested PCR (N- PCR) assays for species-specific ...
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Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription PCR
... ABSTRACT Tilapia are an important group of farmed fish that serve as a significant protein source worldwide. In recent years, substantial mortality of wild tilapia has been observed in the Sea of Galilee and in commercial ...
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Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR
... the nested PCR with the synthetic RNA standard and found that we could increase the sensitivity of detection from 10 3 to 10 molecules by using the touchdown protocol instead of the protocol originally used ...
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Improved Detection of Rhinoviruses in Clinical Samples by Using a Newly Developed Nested Reverse Transcription PCR Assay
... Research Laboratory for Infectious Diseases (LIO), National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands Received 12 August 1998/Returned for modification 28 September 1998/Accepted 8 ...
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Broadly Reactive Nested Reverse Transcription PCR Using an Internal RNA Standard Control for Detection of Noroviruses in Stool Samples
... degree of variability between genotypes, ranging from 2.1 ⫻ 10 6 (Leeds strain) to 39 (Bristol strain) virus particles/ml by nRT-PCR versus 2.1 ⫻ 10 6 virus particles/ml (corresponding to 3.4 ⫻ 10 4 virus ...
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Sensitive Detection of RNA Viruses Associated with Gastroenteritis by a Hanging Drop Single Tube Nested Reverse Transcription PCR Method
... DISCUSSION Nested PCR has rightfully earned a reputation of being at risk of contamination with primary amplification ...one-tube nested RT-PCR described here eliminates contamination by ...
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Evaluation of a Nested Reverse Transcription PCR Assay Based on the Nucleoprotein Gene for Diagnosis of Spontaneous and Experimental Bovine Respiratory Syncytial Virus Infections
... The sensitivities and specificities of different laboratory tech- niques were also analyzed by using a series of clinically and epidemiologically identified field specimens. The sensitivity of n RT-PCR-N was ...
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Enhanced Reverse Transcription PCR Assay for Detection of Norovirus Genogroup I
... or nested PCR. We describe a real-time RT-PCR assay for NV GGI which is based on LNA probe ...of PCR when shorter detection probes are used, due to lower interference during the amplification ...
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Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
... single reverse transcription-polymerase chain reaction (RT- PCR) assay have been made ...as nested, hemi-nested RT-PCR or SYBR green real-time PCR, without using a probe ...
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Development of Reverse Transcription PCR Assays Specific for Detection of Equine Encephalitis Viruses
... sensitive reverse transcription-PCR (RT-PCR) assays were developed for the detection of eastern, western, and Venezuelan equine encephalitis viruses (EEE, WEE, and VEE, ...subsequent ...
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Early Detection of Acute Rhinovirus Infections by a Rapid Reverse Transcription PCR Assay
... (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5 ⴕ -noncoding region, which is chosen ...
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Simultaneous Detection and Typing of Influenza Viruses A and B by a Nested Reverse Transcription PCR: Comparison to Virus Isolation and Antigen Detection by Immunofluorescence and Optical Immunoassay (FLU OIA)
... were incorporated. The assay design was aimed at using an- nealing temperatures of approximately 60°C, which avoid un- specific amplification. Detection limits of the RT-PCR assays. The amplification products of ...
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Stable and Noncompetitive RNA Internal Control for Routine Clinical Diagnostic Reverse Transcription PCR
... three nested multi- plex RT-PCRs in which it was ...three nested multiplex RT-PCRs for the routine detection of nine respiratory viruses in clinical ...
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Detection of human papillomavirus type 16 early gene transcription by reverse transcription PCR is associated with abnormal cervical cytology
... In summary, our preliminary results show that E-mRNA detection is significantly associated with abnormal cytology in women infected with HPV-16 and that the prevalence of HPV-16 E-mRNA increases with advancing ...
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Standardized positive controls for detection of norovirus by reverse transcription PCR
... incorrect PCR mixes, or defective thermal cyclers, positive con- trols are required ...And nested PCR is currently the most sensitive diagnostic assay for the low concen- tration of NoV in ...
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Sensitive and Broadly Reactive Reverse Transcription PCR Assays To Detect Novel Paramyxoviruses
... of reverse transcription-PCR assays for the detection and identification of known and novel paramyxoviruses in clinical ...or nested PCR assay design were used to enhance the breadth of ...
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Quantitative Reverse Transcription Pcr
... AMV Reverse Transcriptase, ...quantitative pcr primer annealing, about the template for in the rna resources for a ...and pcr reaction samples isolated from different types of the temperature in the ...
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5PCR, real-time PCR, reverse transcription, and cloning
... LongRange PCR Kit with a high- yield reverse transcription ...viral reverse transcriptase (AMV) provides highly-sensitive, full-length cDNA synthesis up to ...allows reverse ...
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Reverse transcription PCR detection of hepatitis G virus
... All of the concentrations presented above are final values. Reaction conditions were adapted to the GeneAmp PCR System 9600 thermal cycler (Perkin-Elmer, Foster City, Calif.). Detection of amplification products. ...
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Applications of Competitor RNA in Diagnostic Reverse Transcription PCR
... Detection of RNA viruses by reverse transcription (RT)-PCR has proven to be a useful approach for the diagnosis of infections caused by many viral pathogens. However, adequate controls are required ...
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