Rhoptry neck protein
Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (Pv RON1)
... Rhoptry neck protein 1 (RON1), initially described in Toxoplasma gondii (TgRON1) [16], has been a particu- larly interesting ...sushi protein (ASP), exhibiting a prominent transcriptional peak ...
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Structure of Rhoptry Neck Protein 2 is essential for the interaction in vitro with Apical Membrane Antigen 1 in Plasmodium vivax
... with Rhoptry Neck Protein 2 (RON2) during the host cell invasion [14], and the sequence and structural data from the AMA1 and RON2 proteins suggest that their interaction is highly conserved across ...
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Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations
... The rhoptry is a subcellular organelle of api- complexan parasites. Rhoptry neck proteins (RONs) are secreted by rhoptry for the forma- tion of moving junction (MJ), which plays an important ...
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Size polymorphism and low sequence diversity in the locus encoding the Plasmodium vivax rhoptry neck protein 4 (PvRON4) in Colombian isolates
... The RON protein complex (characterized in P. falcipa- rum) consists of RON2, RON4 and RON5 proteins [14, 17, 19]. Even though the mechanisms regarding func- tion and interaction between the complex’s proteins are ...
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Annotation and characterization of the Plasmodium vivax rhoptry neck protein 4 (Pv RON4)
... called neck and bulb; despite being present in all parasites belonging to the phylum Apicomplexa, their size and number varies according to the target cell being invaded ...a rhoptry [14,15]; such ...
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Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2)
... of 0.6, protein expression was induced by the addition of 0.8 mM IPTG for 4 h at 37 °C. The culture was pel- leted by centrifugation (6000×g, 10 min), resuspended in 25 mL of lysis buffer (10 mM Tris HCl pH 8.0, ...
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Characterization of fine specificity of the immune response to a Plasmodium falciparum rhoptry neck protein, PfAARP
... level increased markedly by day 42 (~1804 pg/ml) post prime immunization (p < 0.05) (Fig. 3a). This increase in secreted IFN- γ levels in splenic cells from protein- immunized mice stimulated with PfAARP may be ...
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Plasmodium falciparum AMA1 Binds a Rhoptry Neck Protein Homologous to TgRON4, a Component of the Moving Junction in Toxoplasma gondii
... the rhoptry necks (2). A P. falciparum rhoptry antigen migrating in the 225- to 240-kDa range has previously been described based on binding of MAb 24C6 ...apical neck region of rhoptries in ...
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Antigenicity and immunogenicity of PvRALP1, a novel Plasmodium vivax rhoptry neck protein
... Genomic DNA was prepared from Korean isolates of P. vivax as described previously [16]. The full-length of pvralp1 comprising amino acid 1 to 675 was amplified from genomic DNA with the forward primer RALP1-F: ...
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The Plasmodium vivax rhoptry neck protein 5 is expressed in the apical pole of Plasmodium vivax VCG 1 strain schizonts and binds to human reticulocytes
... recombinant protein as shown by WB (Figure 3, lane R), were used for determining re- combinant protein preference and/or tropism for human reticulocytes or mature ...
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Pv RON2, a new Plasmodium vivax rhoptry neck antigen
... vivax rhoptry neck protein (named here PvRON2) has been identified in this ...residue-long protein encoded by a single 6,615 bp exon containing a hydrophobic signal sequence towards the ...
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Using Protein-Protein Interaction Network to Prioritize Drug Targets for Malaria
... 13. Richard D, MacRaild CA, Riglar DT et al. Interaction between Plasmodium falciparum apical membrane antigen 1 and the rhoptry neck protein complex defines a key step in the erythrocyte invasion ...
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PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
... Unfortunately, basic P. vivax research has been de- layed mainly due to the parasite’s preference for invad- ing reticulocytes which are difficult to obtain in the high percentages needed for propagating P. vivax in ...
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Using Protein-Protein Interaction Network to Prioritize Drug Targets for Malaria
... 13. Richard D, MacRaild CA, Riglar DT et al. Interaction between Plasmodium falciparum apical membrane antigen 1 and the rhoptry neck protein complex defines a key step in the erythrocyte invasion ...
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The Toxoplasma gondii Rhoptry Kinome Is Essential for Chronic Infection
... Remarkably, nearly complete ablation of chronic infection in the ⌬rop5 and ⌬rop18 knockout strains was associated with only an ~10% reduction in parasite survival in IFN- ␥ -stimulated MEFs in vitro. Therefore, our ...
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Spherical Body Protein 4 Is a New Serological Antigen for Global Detection of Babesia bovis Infection in Cattle
... test in recent decades, it is time-consuming and subjective. Additionally, it cannot be automated, and results are largely influenced by the degree of training of the technician. Re- cently, the competitive ELISA ...
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Genes encoding rhoptry proteins of the rodent malaria parasite <italic> Plasmodium yoelii</italic> YM
... brane protein-1 (PfEMP-1) (Howard and Pasloske, 1993; Newbold and Marsh, ...This protein exhibits a high degree of clonal variation (Roberts, 1993) and has been shown to be encoded by a large gene family ...
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Protective Efficacy of a Toxoplasma gondii Rhoptry Protein 13 Plasmid DNA Vaccine in Mice
... In the past few decades, many T. gondii antigens have been assessed as potential vaccine candidates against toxoplasmosis, in- cluding the surface antigens (SAG1) (9), dense granule proteins (GRA1 to GRA7) (6), microneme ...
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Protective Immunity Induced by Toxoplasma gondii Rhoptry Protein 16 against Toxoplasmosis in Mice
... tent T. gondii strain S48. The use of a live, attenuated strain raises concerns of safety for use in food-producing animals because of the risk of reverting to the wild type with tissue cyst formation. Inactivated ...
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A Glycosylphosphatidylinositol-Anchored Carbonic Anhydrase-Related Protein of Toxoplasma gondii Is Important for Rhoptry Biogenesis and Virulence
... To investigate the localization of TgCA_RP in wild-type tachyzoites, we generated specific antibodies to recombinant TgCA_RP in mice (Fig. 3A, lanes M) and guinea pigs (Fig. 3A, lane GP). Western blot analysis of lysates ...
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