Ribonucleoprotein complexes
Comparison of Cellular Ribonucleoprotein Complexes Associated with the APOBEC3F and APOBEC3G Antiviral Proteins
... The human apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F (APOBEC3F [A3F]) and A3G proteins are effective inhibitors of infection by various retroelements and share ⬃ 50% amino acid sequence identity. ...
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Molecular composition of Ro small ribonucleoprotein complexes in human cells Intracellular localization of the 60 and 52 kD proteins
... small ribonucleoprotein complexes (RoRNPs) are thought to comprise several proteins, including the 60-kD Ro and the 52-kD Ro proteins, and several small RNAs, designated Y ...
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Ambisense gene expression from recombinant rabies virus: random packaging of positive- and negative-strand ribonucleoprotein complexes into rabies virions.
... ambisense ribonucleoprotein complexes (RNPs) into virions demonstrated that the parental 5 * end of the RV genome RNA does not contain putative signals required for incorporation into ...
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Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes
... Results: In this study, a CRISPR/Cas9 facilitated transformation and genome editing method based on in vitro assembled ribonucleoprotein complexes was developed for the filamentous fungus Aspergillus niger. ...
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Maximizing mutagenesis with solubilized CRISPR Cas9 ribonucleoprotein complexes
... sgRNA ribonucleoprotein complexes (RNPs) with minimal toxicity, variable mutagenesis efficiencies depending on targeting sequence, and high mutation ...
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Characterization of ribonucleoprotein complexes containing an abundant polyadenylated nuclear RNA encoded by Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8).
... Infection with Kaposi’s sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is strongly linked to all forms of Kaposi’s sarcoma. We have previously identified two polyadenylated KSHV transcripts that ...
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Influenza Virus-Induced Caspase-Dependent Enlargement of Nuclear Pores Promotes Nuclear Export of Viral Ribonucleoprotein Complexes
... Influenza virus-induced enlargement of nuclear pores leads to increased diffusion limits. IV RNPs are thought to have an approximate molecular mass of up to 5.6 MDa and dimensions of about 30 to 120 nm by 10 to 15 nm ...
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Voltage-Dependent Anion Channel 1 Interacts with Ribonucleoprotein Complexes To Enhance Infectious Bursal Disease Virus Polymerase Activity
... the role of VDAC1 in the progress of IBDV replication, we first examined whether VDAC1 is involved in virus growth. Our results showed that knockdown of VDAC1 impairs IBDV replication, as viral loads, viral RNA ...
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High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
... Cas9–sgRNA complexes from plasmids is not known and may be low given the tendency of ...Cas9 complexes as- sembled in vitro has been reported to yield a higher frequency of edits (Lin et ...Cas9 ...
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Poliovirus infection enhances the formation of two ribonucleoprotein complexes at the 3' end of viral negative-strand RNA.
... negative-strand RNA between positions 2 and 66 (note that positive-strand numbers are used, even though the transcripts are of negative polarity). These proteins are of cellular origin and appear to be modified or ...
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Measles Virus Ribonucleoprotein Complexes Rapidly Spread across Well Differentiated Primary Human Airway Epithelial Cells along F Actin Rings
... ABSTRACT Measles virus (MeV) is a highly contagious human pathogen that con- tinues to be a worldwide health burden. One of the challenges for the study of MeV spread is the identification of model systems that accurately ...
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An Umbraviral Protein, Involved in Long-Distance RNA Movement, Binds Viral RNA and Forms Unique, Protective Ribonucleoprotein Complexes
... ORF3-specific complexes, such as those shown in ...the complexes in fractions with densities between ...the complexes observed in extracts suggest that they correspond to the inclusions ob- served in ...
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Frohn, Anne (2011): Structural and quantitative proteomic analyses of argonaute2-containing ribonucleoprotein complexes. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... protein complexes might be altered but the whole cell pro- teome can undergo significant changes that might have severe effects on the composition of the non-specific background binders associated with the ...
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LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans
... Since the GLD-2 cytoplasmic poly(A) polymerase exhibits mutually exclusive interactions with its accessory factors GLD-3 and RNP-8 (Kim et al. 2009; Nakel et al. 2016), the proteomic data likely report the aggregate ...
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Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus
... We have reported the adaptation of a CRISPR/Cas9 gene editing system that utilizes in vitro assembled Cas9- RNPs coupled with microhomology repair templates [13]. Rather than genetically altering strains to express Cas9 ...
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Antiviral Protein APOBEC3G Localizes to Ribonucleoprotein Complexes Found in P Bodies and Stress Granules
... Members of the APOBEC (apolipoprotein B mRNA-editing enzyme catalytic polypeptide 1-like) family of cytidine deaminases inhibit host cell genome invasion by exogenous retroviruses and endogenous retrotrans- posons. ...
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Ribonucleoprotein complexes of hepatitis delta virus.
... By using UV cross-linking, equilibrium density centrifugation, and immunoprecipitation, we obtained evidence consistent with the interpretation that delta antigen and genomic RNA form a [r] ...
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Influenza C and D Viruses Package Eight Organized Ribonucleoprotein Complexes
... ABSTRACT Influenza A and B viruses have eight-segmented, single-stranded, negative- sense RNA genomes, whereas influenza C and D viruses have seven-segmented genomes. Each genomic RNA segment exists in the form of a ...
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YB-1 Functions as a Porter To Lead Influenza Virus Ribonucleoprotein Complexes to Microtubules
... YB-1 localizes predominantly in the cytoplasm but translo- cates to the nucleus to regulate transcription in response to envi- ronmental stimuli such as DNA-damaging agents, UV irradiation, hyperthermia, and serum ...
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Nuclear import of influenza A viral ribonucleoprotein complexes is mediated by two nuclear localization sequences on viral nucleoprotein
... To study the nuclear import of the influenza genome it was important to first establish a method to purify and fluorescently label the influenza vRNPs in their native state. To purify the influenza vRNPs we used the ...
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