RIG-I/MDA5
Middle East Respiratory Syndrome Coronavirus 4a Protein Is a Double-Stranded RNA-Binding Protein That Suppresses PACT-Induced Activation of RIG-I and MDA5 in the Innate Antiviral Response
... type I interferon ...with RIG-I or MDA5. It inhibited PACT-induced activation of RIG-I and MDA5 but did not affect the activity of downstream effectors such as ...
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Porcine Deltacoronavirus Accessory Protein NS6 Antagonizes Interferon Beta Production by Interfering with the Binding of RIG-I/MDA5 to Double-Stranded RNA
... gene I (RIG-I) and melanoma differentiation gene 5 (MDA5), in the cytoplasm ...recognition, RIG-I and MDA5 are activated, resulting in the recruitment of mitochondrial ...
17
EFTUD2 Is a Novel Innate Immune Regulator Restricting Hepatitis C Virus Infection through the RIG-I/MDA5 Pathway
... (RIG-I, MDA5, TBK1, and IRF3) and JAK-STAT1 ...sensors RIG-I and MDA5 in HCV-infected Huh7 and ...both RIG-I knockdown Huh7 cells and RIG-I-defective ...
11
Human Hemoglobin Subunit Beta Functions as a Pleiotropic Regulator of RIG-I/MDA5-Mediated Antiviral Innate Immune Responses
... the RIG-I-mediated antiviral signaling. RIG-I and MDA5 function as key viral RNA sensors by recognizing different viral RNA species ...by RIG-I, the viral RNA of ...
21
Nonencapsidated 5′ Copy-Back Defective Interfering Genomes Produced by Recombinant Measles Viruses Are Recognized by RIG-I and LGP2 but Not MDA5
... type I interferon (IFN)- inducing abilities that partially depend on the presence of 5= copy-back defective in- terfering genomes ...by RIG-I-like receptors (RLRs) (RIG-I, MDA5, ...
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Expression and Functional Characterization of the RIG-I-Like Receptors MDA5 and LGP2 in Rainbow Trout (Oncorhynchus mykiss)
... in RIG-I- and MDA5-mediated antiviral responses remain patchy and require further ...block RIG-I- and MDA5-elicited signaling via MAVS in mammals since it lacks the N-terminal ...
10
West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
... of RIG-I and ...of RIG-I involves both types of polyubiquitin chain and that NS1 greatly decreased the K63-linked polyubiquitination but not the K48-linked polyubiquitination of ...FLAG- ...
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Distinct RIG-I and MDA5 Signaling by RNA Viruses in Innate Immunity
... recruits RIG-I and MDA5 to the outer membrane of the mitochondria as part of a macromolecular signaling complex that serves to activate downstream interferon-regulatory factors (IRFs) and other ...
11
Sensing and Control of Bluetongue Virus Infection in Epithelial Cells via RIG-I and MDA5 Helicases
... the RIG-I-like receptor (RLR) family (1, ...(2), RIG-I and MDA5 (5, 57, 66), PKR (15, 57), and the newly described TRIF-dependent DexD/H-box helicases ...example, RIG-I is ...
11
The Essential, Nonredundant Roles of RIG-I and MDA5 in Detecting and Controlling West Nile Virus Infection
... by RIG-I remain unknown (32). Moreover, the role of MDA5 in WNV recognition is not well defined, nor have the combinatorial and distinct functions of RIG-I and MDA5 in pathogen ...
10
Murine Coronavirus Induces Type I Interferon in Oligodendrocytes through Recognition by RIG-I and MDA5
... type I IFNs in fibro- blast cells, including studies of 17Cl-1 cells and their insensi- tivity to treatment with type I IFNs (37, 44, 48, ...type I IFN response to MHV ...type I IFN response ...
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Both RIG-I and MDA5 RNA Helicases Contribute to the Induction of Alpha/Beta Interferon in Measles Virus-Infected Human Cells
... nonfunctional RIG-I, although the MDA5 pathway was intact in both cell lines ...that MDA5 is involved in MV-induced expression of IFN-  since forced expression of MDA5, but not ...
8
Kinetic discrimination of self/non-self RNA by the ATPase activity of RIG-I and MDA5
... next MDA5 monomer. This nucle- ates an assembly cascade of other MDA5 molecules on the same dsRNA ...each MDA5 monomer returning back to either re- assemble ...of MDA5 ATPase mutants on an ...
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Amino Acid Requirements for MDA5 and LGP2 Recognition by Paramyxovirus V Proteins: a Single Arginine Distinguishes MDA5 from RIG-I
... in MDA5 and LGP2 but different in RIG-I ...conserved MDA5 or LGP2 amino acids for the RIG-I correlate. For MDA5, 19 mutants were generated containing single or dou- ble ...
5
Human Hemoglobin Subunit Beta Functions as a Pleiotropic Regulator of RIG I/MDA5 Mediated Antiviral Innate Immune Responses
... short polyI:C or long polyI:C for 24 h and the IFN-β mRNA level in cells.. was analyzed using real-time RT-PCR.[r] ...
42
Porcine Epidemic Diarrhea Virus 3C-Like Protease Regulates Its Interferon Antagonism by Cleaving NEMO
... the RIG-I/MDA5 adaptor protein MAVS and the Toll- like receptor 3 (TLR3) adaptor, Toll-IL-1 receptor domain-con- taining adaptor inducing interferon beta (TRIF) (15, 16, 19, 20, ...type I IFN ...
12
Activation of c-jun N-Terminal Kinase upon Influenza A Virus (IAV) Infection Is Independent of Pathogen-Related Receptors but Dependent on Amino Acid Sequence Variations of IAV NS1
... of RIG-I like receptors and of MyD88-dependent ...targeting RIG-I, Mda5, and MAVS, as ...antibody; Mda5, RIG-I, and MAVS, Western blots using antibodies specific to ...
10
Phagocytosis of Picornavirus-Infected Cells Induces an RNA-Dependent Antiviral State in Human Dendritic Cells
... human RIG-I and MDA5 cross-react with their Vero cell (Cercopithecus aethiops, African green monkey) homologues, we focused on mRNA expression of the ISGs IRF7 and ...of RIG-I, ...
8
RIG-I Mediates an Antiviral Response to Crimean-Congo Hemorrhagic Fever Virus
... 2 RIG-I and MDA5 expression is upregulated in CCHFV-infected ...points. RIG-I, MDA5, and MAVS gene expression levels were quantified by qRT-PCR, standardized to  -actin, and ...
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Activation of type I interferon antiviral response in human neural stem cells
... (type I) and λ1 (type III) upon transfection with poly(I:C), which is a synthetic analog of double-stranded RNA for mimicking viral ...sensors RIG-I, MDA5, and TLR3 can be expressed in NSCs ...
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