RNA as the target molecule
TAC-seq: targeted DNA and RNA sequencing for precise biomarker molecule counting
... number of studied molecules and are biased by PCR uneven ampli fication. To overcome ampli fication bias in NGS and to maximize nucleic acid detection sensitivity, unique molecular identi fiers (UMIs, known also as ...
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Proprotein convertase subtilisin/kexin type 9: A new target molecule for gene therapy
... a multi-protein complex called RNA-induced silencing complex (RISC). After incorporation into RISC, the siRNA unwinds, leaving the antisense strand to direct RISC to its target mRNA, for endonucleolytic ...
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A Primary Rna Transcript Molecule Will Contain
... to target tissue specific modified to. Hopefully that a rna transcript molecule attaches ...eucaryotic rna involved in eukaryotes which rna molecule. Rna transcript and ...
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Novel Small-Molecule Inhibitors of RNA Polymerase III
... the target of antifungal ...small molecule inhibitors of RNA polymerase (Pol) III from Saccharomyces ...targeting RNA Pol III in ...of RNA Pol III, confers resistance to the ...
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
... RNA binders and protein ...different target RNAs and inhibit the interactions of protein-RNA complexes have been ...bulged RNA hairpin loop present in the 5' end of all ...
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Can Oligonucleotides Target RNA Splicing to Treat Disease?
... Oligonucleotides Target RNA Splicing to Treat Disease? The human body is phenomenally complex, and we are continually discovering incredible facts about ...of RNA as it is processed in the ...
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Delivery of small molecule and RNA for the treatment of type 1 diabetes and prostate cancer
... Figure 4-6. pshFas-anti-miR-375 transfected hBMSCs combined with hBMSC and PBMC co-cultured exosomes improve the outcome of islet transplantation. (A) Outline of animal study procedure. For islet transplantation, islets ...
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Platinum-seq: High-throughput mapping of small-molecule platinum adducts on cellular RNA
... expression of Saccharomyces cerevisiae treated with cisplatin and a second novel platinum(II) compound (azaplatin), using data acquired from the work in Chapter 2. Chapter 4 describes recent efforts to implement ...
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Engineering dynamic cell cycle control with synthetic small molecule-responsive RNA devices
... cleaving RNA regulatory elements [26], where cleavage can result in transcript degradation when placed in the 3′-untranslated region (3′ UTR) of the target gene of ...an RNA aptamer [27, 28] to the ...
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Identification of Kinase Inhibitors that Target Transcription Initiation by RNA Polymerase II
... small molecule inhibitors that have delineated multiple regulatory steps in site-specific initiation and elongation of RNA synthesis by multiple forms of RNA polymerase ...common target of ...
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The RNA strategy. RNA as a tool and target in human disease diagnosis and therapy.
... early detection, sensitivity and ease of translatability from model systems to humans. Indeed in the last ten years, alteration of miRNA levels and/or correlation of miRNA expression with clinical parameters (such as ...
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RNA metabolism is the primary target of formamide in vivo
... As opposed to budding yeast, which can cope with 3% v/v, here we show that fission yeast cells are only able to grow up to saturation in the presence of ~2% v/v formamide. Nevertheless, the goodness of fit to a ...
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Specialized Plant Metabolism Characteristics and Impact on Target Molecule Biotechnological Production.
... engineered cultures, as well as heterologous expression in microbial platforms, is discussed. Data to date strongly suggest that attaining full potential of these biotechnology production strategies requires being able ...
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Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions
... Fluorescence labeling Labeling procedures followed the standard protocols recommended by the supplier of reactive fluorophores (http:// www.atto-tec.com). Slight alterations in molar ratios of dye and protein/antibody ...
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Single molecule pull down of double strand RNA binding proteins
... single molecule fluorescence The functions of dsRBPs are implicated in diverse cellular pathways including micro RNA, RNA editing, antiviral signaling, mRNA transport and alternative ...single ...
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P68 RNA helicase as a molecular target for cancer therapy
... MicroRNAs(miRNAs) are small non-coding RNAs that can affect cell development through regulating protein expression or messenger RNA synthesis [27]. The primary miRNA (pri-miRNA) is cleaved by the nuclear RNase III ...
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Extracellular RNA: mechanisms of it’s transporting into target cells
... Currently, extracellular RNA discovery is ground-break- ing in molecular biology. The evidence of detecting exRNAs in biofluids and carriers, including EVs, HDL and Ago2, sheds new light on the field of ...
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Evaluation of off target and on target scoring algorithms and integration into the guide RNA selection tool CRISPOR
... Acknowledgements We thank Donna Karolchik for copy editing of the manuscript and Jean-Baptiste Penigault for the design of the CRISPOR site. We thank Hari Jay for making his implementation of the MIT guide specificity ...
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Identification of alsterpaullone as a novel small molecule inhibitor to target group 3 medulloblastoma
... This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and ...
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Identification and characterization of the intercellular adhesion molecule-2 gene as a novel p53 target
... downstream target genes, many of which are involved in apoptosis, cell-cycle arrest, and DNA repair [1, ...p53 target genes is key to understanding the role of p53 in ...
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