RNA Dependent RNA Polymerase
Membrane Association of the RNA-Dependent RNA Polymerase Is Essential for Hepatitis C Virus RNA Replication
... (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), belongs to a class of integral membrane proteins termed tail-anchored ...HCV RNA replication ...
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Norovirus Proteinase-Polymerase and Polymerase Are Both Active Forms of RNA-Dependent RNA Polymerase
... viral RNA-dependent RNA polymerase (RdRp) domains: ProPol (a precursor comprised of both the proteinase and polymerase) and Pol (the mature ...
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Synthesis of novel products in vitro by an RNA-dependent RNA polymerase.
... RNA-dependent RNA polymerase from turnip crinkle virus-infected turnip transcribes both strands of a virus-associated satellite RNA, sat-RNA C (356 bases), in ...minus-strand ...
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Binding mechanism of remdesivir to SARS-CoV-2 RNA dependent RNA polymerase
... virus RNA-dependent RNA polymerase ...this polymerase based on a previously available structure of SARS-CoV NSP12 RdRp (with a sequence identify of ...
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Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase
... (FCV) RNA-dependent RNA polymerase ...proteinase- dependent processing of Pro-Pol in the interdomain region preceding Val-1235 was not observed in the presence of a catalytically active ...
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The Crystal Structure of a Cardiovirus RNA-Dependent RNA Polymerase Reveals an Unusual Conformation of the Polymerase Active Site
... picornaviral RNA-dependent RNA polymerase (RdRp) 3D pol , responsible for RNA genome replication, has been exten- sively studied, and there is a growing body of information avail- able ...
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Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase
... Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene ...enzyme RNA-dependent RNA polymerase ...
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Alfalfa Mosaic Virus Coat Protein Bridges Rna and Rna-dependent Rna Polymerase in Vitro
... (RNA-dependent RNA polymerase) protein under the control of the polyhedron promoter were used to infect insect ...the RNA-dependent RNA polymerase (RdRp) ...an ...
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Mutational Analysis of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase
... an RNA-dependent RNA polymerase (RdRp) ...direct RNA replication via both primer-dependent (elongative) and primer- independent (de novo) ...for RNA synthesis, suggesting ...
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Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro.
... virus RNA-dependent RNA polymerase, purified from virion ribonucleoprotein particles and from which endogenous genomic RNA (vRNA) has been depleted by treatment with micrococcal ...
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Norovirus RNA-Dependent RNA Polymerase Is Phosphorylated by an Important Survival Kinase, Akt
... Viruses commonly use host cell survival mechanisms to their own advantage. We show that Akt, an important signaling kinase involved in cell survival, phosphorylates the RNA-dependent RNA ...
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Characterization of an RNA-dependent RNA polymerase activity associated with measles virus.
... Figure 1.35 1.30 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest RESULTS The Edmonston B strain of measles virus grown in Vero cells showed RNA-dependent RNA polymerase[r] ...
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Mutation of lysine residues in the nucleotide binding segments of the poliovirus RNA-dependent RNA polymerase.
... 3D RNA-dependent RNA polymerase contains two peptide segments previously shown to cross-link to nucleotide substrates via lysine ...catalyze RNA chain elongation in ...of RNA ...
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RNA-Dependent RNA Polymerase in Nuclei of Cells Infected with Influenza Virus
... In this communication we describe the appearance and properties of RNA-dependent RNA polymerase activity in nuclei of cells infected with influenza virus, and compare the characteristics[r] ...
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Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... that RNA polymer- ase II (Pol II) transcription and RNA processing are coupled in vivo (11, 12, ...and RNA processing are influenced not only by cellular factors such as CTD kinases and phosphatases ...
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Template Requirements for RNA Synthesis by a Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase
... direct RNA synthesis by HCV NS5B. The lengths of each RNA in nucleotides (nt) are shown in ...autoradiograms. RNA synthesized from SLD3 comigrated with a preparation of SLD3 RNA that was end ...
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Oligomerization and Cooperative RNA Synthesis Activity of Hepatitis C Virus RNA-Dependent RNA Polymerase
... Due to the essential role of NS5B protein in the HCV replication and infection process (22), this enzyme has been studied extensively. In addition, the crystal structure of purified HCV NS5B has been solved at high ...
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Characterization of an RNA-dependent RNA polymerase activity associated with La France isometric virus.
... a second family of dsRNA viruses known as the Partiviridae replicate by way of a semiconservative mechanism whereby nascent transcripts remain hybridized to the parental template while the complementary parental strand ...
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Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro.
... The largest product RNA synthesized by the fraction V polymerase plus host factor or by the fraction III polymerase was twice the size of the poliovirion RNA template.. Dimer-sized produ[r] ...
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Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor
... NITD107 weakly but selectively binds to DENV RdRp. We performed a surface plasmon resonance (SPR) analysis to demon- strate the binding of NITD107 to DENV RdRp. SPR measure- ments were done on a Biacore 3000 (Uppsala, ...
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