RNA-dependent RNA polymerase complex
Synthesis of novel products in vitro by an RNA-dependent RNA polymerase.
... of RNA on an RNA template occurs during RNA-RNA recombination, and since mutations that affect the synthesis of L-RNA also affect RNA recombi- nation, it is possible that some ...
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Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... virus RNA synthesis. By being in a complex with active, engaged Pol IIo, influenza virus could make use of the locally higher con- centrations of capped pre-mRNAs and preassembled splicing ...virus ...
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MECHANISTIC CHARACTERIZATION AND DESIGNING POSSIBLE MOLECULAR LIGAND INTERACTIONS WITH RDRP FROM CHIKV
... The RNA dependent RNA polymerase protein was selected from Chikungunya virus and the sequence of this protein was taken from NCBI and the structure was modelled using the bioinformatics tool ...
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The contribution of the RNA dependent RNA polymerase to genetic recombination in enteroviruses
... heteropolymeric RNA primer-‐template substrate (figure ...radiolabelled RNA that forms a 6-‐base pair duplex flanked by two, 4-‐nt 5’-‐overhangs (Arnold and Cameron, ...complementary RNA is ...
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De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase
... initiated RNA synthesis de novo on homopolymeric templates in a template-specific ...novo RNA synthesis generally initiates with a purine as the starting ...of RNA synthesis or runoff polymerization ...
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Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers.
... for RNA replication ...RNAs: RNA 1 (3.2 kb), RNA 2 (2.8 kb), and RNA 3 ...the RNA 1-encoded 109-kDa 1a pro- tein, the RNA 2-encoded 94-kDa 2a protein, and a host pro- tein ...
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Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase
... other linking ␣ -helix 14 with 15. The thumb domain interacts with the N-terminal extension via ␣ -helix 12 and the loop containing residues 418 to 435 which link helix 12 and 13. The part of the loop containing residues ...
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NusG Dependent RNA Polymerase Pausing and Tylosin Dependent Ribosome Stalling Are Required for Tylosin Resistance by Inducing 23S rRNA Methylation in Bacillus subtilis
... Regulation of B. subtilis tlrB expression combines tylosin-dependent ribosome stall- ing, transcription attenuation, and translation attenuation mechanisms. The mecha- nism regulating B. subtilis tlrB expression ...
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Biochemical characterization of the fidelity of poliovirus RNA dependent RNA polymerase
... of RNA viruses ...to RNA sequence variation are ...tautomerization, RNA editing and ...enzyme RNA complex in the presence of incorrect base may suggest that recombination is induced by ...
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Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor
... flavivirus RNA-dependent RNA poly- merase (RdRp), located at the C-terminal two-thirds of nonstruc- tural protein 5 (NS5), is an attractive target for antiviral develop- ment ...in complex ...
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Intramolecular and Intermolecular Uridylylation by Poliovirus RNA-Dependent RNA Polymerase
... single-stranded RNA viral genomes are relatively small templates that encode information for complex viral replication cycles and thus require highly efficient utiliza- tion of limited coding ...an ...
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Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase
... of polymerase derivatives beginning with Val-1235, a residue con- served among all calicivirus polymerases and located prior to the established amino termini of RHDV and SV polymerases ...ubiquitin- ...
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RNA-Dependent RNA Polymerase in Nuclei of Cells Infected with Influenza Virus
... In this communication we describe the appearance and properties of RNA-dependent RNA polymerase activity in nuclei of cells infected with influenza virus, and compare the characteristics[r] ...
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Beclabuvir can inhibit the RNA-dependent RNA polymerase of newly emerged novel coronavirus (SARS-CoV-2)
... Recent emergence of novel coronavirus (SARS-CoV-2) all over the world has resulted more than 33,106 global deaths. To date well-established therapeutics modules for infected patients are unknown. In this present ...
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CONSERVED FUNCTIONAL DOMAINS OF THE RNA-POLYMERASE-III GENERAL TRANSCRIPTION FACTOR BRF
... A TBP- containing multiprotein complex (TIF-IB) mediates tran- scription specificity of murine RNA polymerase I. Transcrip- tion by RNA polymerase III. The TPR snap heli[r] ...
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RNA-dependent RNA polymerase activity in coronavirus- infected cells.
... Percentage composition and relative molar concentration of polyadenylated viral RNA species synthesized in vivoa RNA Relative Percentage molar species mol Downloaded from http://jvi.asm.[r] ...
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Infectious Bursal Disease Virus Capsid Protein VP3 Interacts both with VP1, the RNA-Dependent RNA Polymerase, and with Viral Double-Stranded RNA
... VP3- RNA interaction seemed most ...the RNA was indeed of IBDV origin and that it contained both positive and negative strands of each of the two genome ...minus RNA strand, the results indicated ...
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Rational Attenuation of a Morbillivirus by Modulating the Activity of the RNA-Dependent RNA Polymerase
... was readily detected by UV microscopy as early as 8 hpi. Both intracytoplasmic inclusions and membrane-associated EGFP autofluorescence were observed in unfixed living cells (data not shown). Fluorescence was detectable ...
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Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase
... The results showed that, RdRP did induce a change in RNAi in the cells. The inhibition observed with the enzyme was significantly different from the ones without. This suggests a possible enhancement of RNAi by the ...
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journal.ppat.1001163.PDF
... of RNA synthesis by amiloride is dose ...of RNA synthesis of wild type virus treated with different concentrations of ...and RNA was extracted 48 hours after ...
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