RNA polymerase II CTD
Shah, Nilay (2017): Functional analysis of Tyrosine-1 of mammalian RNA polymerase II CTD. Dissertation, LMU München: Fakultät für Biologie
... Pol II levels over the gene body, while Pol II levels at the 5’ end were ...Pol II failed to escape pausing and transit to productive ...Pol II from entering into productive ...Pol II ...
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Genetic Interactions of Spt4-Spt5 and TFIIS With the RNA Polymerase II CTD and CTD Modifying Enzymes in Saccharomyces cerevisiae
... of RNA polymerase II; and (3) protein kinases that phosphorylate the CTD and a phosphatase that dephosphory- lates ...the CTD; affect the Kin28, Bur1, or Ctk1 CTD kinases; and ...
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Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations.
... WTm), then viability is restored. Together these results indicate that both the number and position of different phosphorylation sites are im- portant for CTD function. The pr[r] ...
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Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... the CTD was sufficient for the interaction between Pol II and influenza virus ...Pol II with the trimeric influenza virus polymerase complex or with the indi- vidual polymerase ...virus ...
7
Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
... genomic RNA is sensitive to ...antigenomic RNA. In contrast, Macnaughton et al. obtained data that antigenomic RNA syn- thesis seemed resistant to amanitin, but we consider that the signals they ...
10
Phosphorylation of Serine 177 of the Small Hepatitis Delta Antigen Regulates Viral Antigenomic RNA Replication by Interacting with the Processive RNA Polymerase II
... antigenomic RNA replication was examined to test the role of RNAP II CTD phosphorylation in this ...antigenomic RNA and SHDAg ...total RNA was extracted and analyzed by Northern ...
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RNA polymerase II pausing during development
... Pol II pausing is a late step during the initiation stage of ...Pol II and basal transcription factors to an accessible promoter to form the preinitiation complex (PIC) [for a recent review see Luse (Luse, ...
5
Genetic Interactions With C-Terminal Domain (CTD) Kinases and the CTD of RNA Pol II Suggest a Role for ESS1 in Transcription Initiation and Elongation in Saccharomyces cerevisiae
... the CTD compensates for the loss of ...two CTD kinases (Srb10 and Ctk1), ports a transcription-based model for mitotic regulation these data suggest that one role of Ess1 isomerization in which Ess1 ...
14
Chapman, Rob (2003): A Functional Analysis of the RNA Polymerase II Large Subunit C-Terminal Domain. Dissertation, LMU München: Fakultät für Biologie
... the RNA polymerase II large subunit CTD. The CTD has been shown to be essential at almost every stage of in vivo transcription: initiation, elongation, termination and pre-mRNA ...Pol ...
133
A Role for Histone Chaperones in Regulating RNA Polymerase II
... RNAPII transcribes mRNA from a DNA template in all protein-encoding genes. Transcription relies on a coor- dinated network of histone modifying enzymes and histone chaperones to allow elongating RNAPII to traverse the ...
10
Schueller, Roland (2013): Insights into the RNA Polymerase CTD code. Dissertation, LMU München: Fakultät für Biologie
... mammalian CTD consensus and non-consensus repeats is probably not entirely essential for life but rather ‘optimized’ for efficient function or other, as yet unknown, purposes that could come along with a survival ...
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RNA Polymerase II during Transcript Elongation: deaiing with DNA damage and staying phosphorylated
... the CTD may stabilise the interaction between complexes which recognise the efficiency and positioning elements ...phosphorylated CTD conferred more cleavage activity than the addition of hypophosphorylated ...
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Lux, Christoph (2005): Untersuchungen zur transkriptionellen Regulation des Proto-Onkogens c-myc. Dissertation, LMU München: Medizinische Fakultät
... der RNA-Polymerase II konditional exprimiert ...von CTD-Deletionen auf die Initiation der Transkription und das Promotor-proximale Pausieren untersucht ...verkürzte CTD in ...
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Interaction of RNA polymerase II and the small RNA machinery affects heterochromatic silencing in Drosophila
... with RNA Pol II (8WG16) CTD antibody (Figure 6), but not with antibodies against the activated ...of RNA Pol II(A5)/+; dcr-1(Q1147X) compared to wild type (Additional file ...of ...
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Bioinformatics resources for deciphering the biogenesis and action pathways of plant small RNAs
... hairpin-like structures could form within the specific re- gions of the pri-miRNAs. These local hairpin structures are the featured substrates of Dicer-like 1 (DCL1). Followed by the DCL1-mediated two-step cropping in ...
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Environmental adaptability and stress tolerance of Laribacter hongkongensis: a genome-wide analysis
... (RpoE): RNA polymerase sigma-E factor; σ 28 (FliA): RNA polymerase sigma factor for flagellar operon; σ 32 (RpoH): RNA polymerase sigma-32 factor; σ 38 (RpoS): RNA ...
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Compromised RNA polymerase III complex assembly leads to local alterations of intergenic RNA polymerase II transcription in Saccharomyces cerevisiae
... using RNA extracted from the wild type and tfc6 ...separate RNA species, RT-PCR primers were designed to amplify cDNA produced from the normal FAR3 mRNA as controls (within the coding sequence), along with ...
16
Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps
... many), mRNA was isolated by Fast Track 2.0 kit (Invitro- gen, Karlsruhe, Germany). Concentration of purified RNA was determined by measuring absorbance at 260 nm. Five µg RNA was separated on an 1% agarose ...
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A Divergent Transcription Factor TFIIB in Trypanosomes Is Required for RNA Polymerase II-Dependent Spliced Leader RNA Transcription and Cell Viability
... SL RNA gene ...SL RNA gene ...Pol II or TbTBP was removed from the extract during the immunodepletion of TbTFIIB ...SL RNA gene promoter ...to RNA Pol II, as robust transcription ...
8
RNA-Dependent DNA Polymerase Activity of RNA Tumor Viruses II. Directing Influence of RNA in the Reaction
... The polymerase catalyzes the synthesis of DNA in the presence of four deoxynucleoside triphosphates, Mg2+, and a variety of RNA templates including those isolated from avian myeloblastos[r] ...
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