RNA triphosphatase
Mutational analysis of the RNA triphosphatase component of vaccinia virus mRNA capping enzyme.
... Our report of experiments with two vaccinia virus triphos- phatase-defective mutants is (to our knowledge) the first in which a purified capping enzyme catalyzed GMP transfer to the 5 9 triphosphate terminus of an ...
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Nanomolar Inhibitors of Trypanosoma brucei RNA Triphosphatase
... the RNA triphosphatase component of mimivirus capping enzyme (MimiCE) (18); (iii) the RNA triphosphatase component of the baculovirus Autographa californica nucleopolyhedrovirus (AcNPV) ...
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RNA Triphosphatase Component of the mRNA Capping Apparatus of Paramecium bursaria Chlorella Virus 1
... nascent RNA is hydrolyzed to a diphos- phate by RNA triphosphatase (RTP), (ii) the diphosphate end is capped with GMP by GTP:RNA guanylyltransferase, and (iii) the GpppN cap is methylated by ...
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The LEF-4 Subunit of Baculovirus RNA Polymerase Has RNA 5′-Triphosphatase and ATPase Activities
... the triphosphatase domain; therefore, we considered the possibility that the ts phenotype was due to a defect in mRNA ...the RNA triphos- phatase activity of the mutant relative to the wild-type protein at ...
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A Novel Role for Cet1p mRNA 5′-Triphosphatase in Promoter Proximal Accumulation of RNA Polymerase II in Saccharomyces cerevisiase
... N-terminal triphosphatase and C-terminal guanylyltransferase domains (Mizumoto and Kaziro 1987; McCracken et ...separate RNA triphosphatase (Cet1p) and guanylyltransferase (Ceg1p) (Itoh et ...
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Roles of LEF-4 and PTP/BVP RNA Triphosphatases in Processing of Baculovirus Late mRNAs
... was RNA and that it released phosphates from the 5 ⬘ end of RNA (13, ...metazoan RNA cap- ping enzymes, thus confirming its specificity for RNA sub- strates ...yeast RNA ...
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Brome Mosaic Virus 1a Nucleoside Triphosphatase/Helicase Domain Plays Crucial Roles in Recruiting RNA Replication Templates
... preformed RNA replication complexes are shifted to a nonpermissive temperature, a strong temper- ature-sensitive insertion mutation near the 1a NTPase domain blocks further synthesis of positive- and ...
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RNA 5′-Triphosphatase, Nucleoside Triphosphatase, and Guanylyltransferase Activities of Baculovirus LEF-4 Protein
... nascent RNA is hydrolyzed to a diphosphate by RNA triphosphatase; (ii) the diphosphate end is capped with GMP by GTP:RNA guanylyltransferase; and (iii) the GpppN cap is methylated by ...
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A Yeast-Based Genetic System for Functional Analysis of Viral mRNA Capping Enzymes
... separate triphosphatase (Cet1p), guanylyltransferase (Ceg1p), and methyltransferase (Abd1p) gene products (22, 31, 43, ...the triphosphatase, guanylyltransferase, and methyltransferase active sites (21, 27, ...
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RNA 5′-Triphosphatase Activity of the Hepatitis E Virus Helicase Domain
... documented RNA 5 ⬘ -triphosphatases can be grouped into three classes according to the catalytic mecha- nisms they ...The RNA triphosphatases from RNA viruses are helicases or helicase-like proteins ...
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Characterization of a Baculovirus-Encoded RNA 5′-Triphosphatase
... AcNPV RNA triphosphatase. The native structure of AcNPV RNA triphosphatase was analyzed by sed- imentation through a 15 to 30% glycerol gradient containing ...of RNA ...
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Proteins of a Polyhedral Cytoplasmic Deoxyvirus
... McAUSLAN Roche Ii-stitute of Molecular Biology, Nutley, New Jersey 07110 The adenosine triphosphatase associated with frog virus 3 shows high specificity for ATP or deoxyadenosine tripho[r] ...
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The Serine Protease and RNA-Stimulated Nucleoside Triphosphatase and RNA Helicase Functional Domains of Dengue Virus Type 2 NS3 Converge within a Region of 20 Amino Acids
... membranes, which enhanced the cleavage efficiency of 2B-3 site (9). The precursor products were analyzed for the ability to undergo processing by cis cleavage of the 2B-3 site. Using four plasmid constructs, ...
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Detergent-inhibited, heat-labile nucleoside triphosphatase in cores of avian myeloblastosis virus.
... Detergent-Inhibited, Heat-Labile Nucleoside Triphosphatase in Cores of Avian Myeloblastosis Virus KAJ FRANK JENSENt Department of Biochemistry, College of Agricultural and Life Sciences,[r] ...
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Hepatoprotective Effects of Propolis Extract: A Biochemical and Histopathological Approach
... Hepatoprotective potential of propolis extract on the activities of hepatorenal adenosine triphosphatase, acid phosphatase and alkaline phosphatase against carbon tetrachloride administe[r] ...
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SALINITY INDUCED ADENOSINE TRIPHOSPHATASE ACTIVITY IN GERMINATING RICE SEEDS
... During early hours of germination, salinity decreased ATPase activity in both endosperms and embryoaxes but thereafter sharp increase in activity was observed -over contro[r] ...
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Double-stranded RNA bacteriophage phi 6 protein P4 is an unspecific nucleoside triphosphatase activated by calcium ions.
... was used in the biochemical measurements. In all the cases when the NC and the multimeric P4 were assayed and the NTPase activity was normalized to the amount of P4 (densi- tometry based on image analysis of SDS-PAGE), ...
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Cotherapy of Tiron and selenium against vanadium induced toxic effects in lactating rats
... Blood was collected and serum was isolated for various blood biochemical assays directly from the heart by puncturing the retro-orbital venous sinus. Blood sugar (22) aspartate aminotransferase (AST), alanine ...
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Properties of chicken liver membrane-associated thiamine triphosphatase
... Braun [21] разграничили ТТРаз- ную и Mg 2+ -зависимую АТPазную (NTPазную) активность мембран мозга крысы, используя β-γ-метиленовый аналог ТТР, который снижал скорость [r] ...
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Inhibition of virion-associated reverse transcription by nucleoside triphosphatase in avian myeloblastosis virus.
... It appears that nucleoside triphosphatase associated with virions released by leukemic my- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest crease in RDDP activity demon[r] ...
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