S-adenosylmethionine
Metabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle
... S-Adenosylmethionine (SAM) is one of the most widely used cofactors for group transfer reactions involved in various metabolic processes, and it serves as the main methyl donor for methylation of DNA, ...
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Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway
... To better understand the contributions of methionine biosynthesis to the vanillate pathway, a second supple- mentation experiment was performed, this time with the direct precursor to methionine, l-homocysteine (Fig. ...
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Defective cystathionine beta synthase regulation by S adenosylmethionine in a partially pyridoxine responsive homocystinuria patient
... by S-adenosylmethionine, contrary to a threefold stimulation in control fibroblast ...different S-adenosylmethionine concentrations (0–200 m ...by S-adenosylmethionine in the ...
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Improved NOE fitting for flexible molecules based on molecular mechanics data – a case study with S adenosylmethionine
... of S-adenosylmethionine presents an excellent example for a detailed evaluation of conformational equilibria by NMR NOE experiments in combination with molecular ...
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Gene expression modifications in the liver caused by binge drinking and S-adenosylmethionine feeding. The role of epigenetic changes
... Male Wistar rats from Harleco (Hollister, CA), weighing 250–300 g, were used. The rats from a previously reported study [1] (number 3–4/group) were fed acutely an alcohol bolus of ethanol by gavage (6 g/kg body weight, ...
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GADD45 induction by S-adenosylmethionine inhibits hepatocellular carcinoma cell proliferation during acute ischemia-hypoxia
... Growth arrest DNA damage-inducible gene 45β (GADD45 β ), which influences cell growth, apoptosis and cellular response to DNA damage, is downregulated in hepatocellular carcinoma (HCC). S-adenosylmethionine ...
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Overexpression of S adenosylmethionine decarboxylase (SAMDC) in early Xenopus embryos induces cell dissociation and inhibits transition from the blastula to gastrula stage
... Int J Do, BioI 42 675 686 (1998) Oligillal A/tide Overexpression of S adenosylmethionine decarboxylase (SAM DC) in early Xenopus embryos induces cell dissociation and inhibits transition from the blas[.] ...
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Homology modeling of S adenosylmethionine synthase present in E maximum as a drug target and its docking with 1 monocaproin
... enzyme S-Adenosylmethionine synthase present in ...the S-Adenosylmethionine synthase is modeled using homology modeling techniques and docking with 1-monocaproin, found the binding energy as ...
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Genomewide Analysis of Mode of Action of the S-Adenosylmethionine Analogue Sinefungin in Leishmania infantum
... ABSTRACT To further our understanding of one-carbon metabolism in the pro- tozoan parasite Leishmania, we conducted genomic screens to study how the parasite responded to sinefungin (SNF) selection. SNF is a structural ...
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Protective effect of S adenosylmethionine on hepatic ischemia reperfusion injury during hepatectomy in HCC patients with chronic HBV infection
... S-adenosylmethionine (SAMe or AdoMet) is a natural substance and serves as a reduced glutathione, an en- dogenous methyl donor and ATP precursor in various tissues [4], and plays a crucial role in liver ...
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S-Adenosylmethionine Inhibits the Growth of Cancer Cells by Reversing the Hypomethylation Status of c-myc and H-ras in Human Gastric Cancer and Colon Cancer
... S-Adenosylmethionine (SAM) is a methyl donor for numerous methylation reactions and acts as an inhibitor of intracellular demethylase activity, which results in hypermethylation of DNA [35]. Zhao et al. ...
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Characterization of the Candida albicans Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an S-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis
... ABSTRACT Amino acids are key sources of nitrogen for growth of Candida albi- cans. In order to detect and take up these amino acids from a broad range of differ- ent and changing nitrogen sources inside the host, this ...
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Overexpression of S adenosylmethionine decarboxylase impacts polyamine homeostasis during development of Dictyostelium discoideum
... the S-adenosylmethionine decar- boxylase (samdc) gene or treating the cells with methylglyoxal-bis (guanylhydrazone) (MGBG), an inhibitor of ...the S-phase of the cell ...
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Engineered Pichia pastoris for enhanced production of S-adenosylmethionine
... S-adenosylmethionine (SAM) is a metabolite of the methionine metabolism. In diverse living organisms, it is known to mediate three important metabolic path- ways, viz., transmethylation, trans-sulphuration, ...
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Identification and Characterization of the Chlamydia trachomatis L2 S Adenosylmethionine Transporter
... the S-adenosylmethionine (SAM) syn- thetase required for synthesis of SAM, and to sahH, which encodes the S-adenosylhomocysteine (SAH) hydrolase required for detoxification of SAH formed after the ...
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Pneumocystis Encodes a Functional S-Adenosylmethionine Synthetase Gene
... S-Adenosylmethionine (AdoMet) synthetase (EC 2.5.1.6) is the enzyme that catalyzes the synthesis of AdoMet, a molecule important for all cellular organisms. We have cloned and characterized an AdoMet ...
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S-Adenosylmethionine regulates apoptosis and autophagy in MCF-7 breast cancer cells through the modulation of specific microRNAs
... In order to confirm the ability of the sulfonium com- pound to regulate miRNA expression in other breast cancer cells, we performed a qRT-PCR analysis after 72 h of 500 µM AdoMet tre[r] ...
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Vitamin B12 insufficiency induces cholesterol biosynthesis by limiting s-adenosylmethionine and modulating the methylation of SREBF1 and LDLR genes
... Specifically, we have demonstrated that low vitamin B 12 (a) is associated and independently predicted higher total cholesterol, LDL cholesterol, and cholesterol-to-HDL ratio in serum; ([r] ...
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Vitamin B12 insufficiency induces cholesterol biosynthesis by limiting s adenosylmethionine and modulating the methylation of SREBF1 and LDLR genes
... Specifically, we have demonstrated that low vitamin B12 a is associated and independently predicted higher total cholesterol, LDL cholesterol, and cholesterol-to-HDL ratio in serum; b in[r] ...
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eth-1, the Neurospora crassa Locus Encoding S-Adenosylmethionine Synthetase: Molecular Cloning, Sequence Analysis and in Vivo Overexpression
... crassa DNA methyltransferase mutants have re- cently been performed (FOSS et al. As an alternative approach to study the consequences of abnormal SAdomet metabolism on p[r] ...
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