S-adenosylmethionine (AdoMet)
Identification and Characterization of the Chlamydia trachomatis L2 S Adenosylmethionine Transporter
... The methylation cycle is under reductive evolutionary pressure in Chlamydia. We recently showed that the conserved dimethyl- transferase KsgA plays an important role in Chlamydia biology (10). KsgA transfers two methyl ...
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Genomewide Analysis of Mode of Action of the S-Adenosylmethionine Analogue Sinefungin in Leishmania infantum
... of S-adenosylmethionine (AdoMet), a key methyl group donor to a number of biomol- ...the AdoMet transporter (AdoMetT1) by intergenic recombination as a crucial loss-of-function marker for SNF ...
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Improved NOE fitting for flexible molecules based on molecular mechanics data – a case study with S adenosylmethionine
... valuable. S-Adenosylmethionine (AdoMet or SAM) is one such molecule where accurate conformational data are critical to understanding its biological and chemical ...
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Engineered Pichia pastoris for enhanced production of S-adenosylmethionine
... S-adenosylmethionine (SAM) is a metabolite of the methionine metabolism. In diverse living organisms, it is known to mediate three important metabolic path- ways, viz., transmethylation, trans-sulphuration, ...
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Overexpression of S adenosylmethionine decarboxylase impacts polyamine homeostasis during development of Dictyostelium discoideum
... the S-adenosylmethionine decar- boxylase (samdc) gene or treating the cells with methylglyoxal-bis (guanylhydrazone) (MGBG), an inhibitor of ...the S-phase of the cell ...
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Gene expression modifications in the liver caused by binge drinking and S-adenosylmethionine feeding. The role of epigenetic changes
... Male Wistar rats from Harleco (Hollister, CA), weighing 250–300 g, were used. The rats from a previously reported study [1] (number 3–4/group) were fed acutely an alcohol bolus of ethanol by gavage (6 g/kg body weight, ...
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Homology modeling of S adenosylmethionine synthase present in E maximum as a drug target and its docking with 1 monocaproin
... (http://www.uniprot.org) S- adenosylmethionine synthase is a kind of enzyme involved in various biological process such as production of secondary metabolites, gene transcription and cell proliferation and ...
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GADD45 induction by S-adenosylmethionine inhibits hepatocellular carcinoma cell proliferation during acute ischemia-hypoxia
... Growth arrest DNA damage-inducible gene 45β (GADD45 β ), which influences cell growth, apoptosis and cellular response to DNA damage, is downregulated in hepatocellular carcinoma (HCC). S-adenosylmethionine ...
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Metabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle
... S-Adenosylmethionine (SAM) is one of the most widely used cofactors for group transfer reactions involved in various metabolic processes, and it serves as the main methyl donor for methylation of DNA, ...
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Overexpression of S adenosylmethionine decarboxylase (SAMDC) in early Xenopus embryos induces cell dissociation and inhibits transition from the blastula to gastrula stage
... Int J Do, BioI 42 675 686 (1998) Oligillal A/tide Overexpression of S adenosylmethionine decarboxylase (SAM DC) in early Xenopus embryos induces cell dissociation and inhibits transition from the blas[.] ...
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Defective cystathionine beta synthase regulation by S adenosylmethionine in a partially pyridoxine responsive homocystinuria patient
... by S-adenosylmethionine, contrary to a threefold stimulation in control fibroblast ...different S-adenosylmethionine concentrations (0–200 m ...by S-adenosylmethionine in the ...
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Pneumocystis Encodes a Functional S-Adenosylmethionine Synthetase Gene
... size AdoMet de novo and suggest that the organism does not need to rely on exogenous, host-derived AdoMet for ...of AdoMet synthetase encoded by different but closely related genes are present in ...
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Vitamin B12 insufficiency induces cholesterol biosynthesis by limiting s adenosylmethionine and modulating the methylation of SREBF1 and LDLR genes
... The AdoMet-to-AdoHcy ratio is known as a marker of DNA MP due its direct relationship to the availability of methyl groups capable of methylation. We confirmed DNA hypomethylation in these conditions in both ...
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Mutations in the Drosophila melanogaster Gene Encoding S-adenosylmethionine Suppress Position-Effect Variegation
... In Drosophila melanogaster, the study of trans-acting modifier mutations of positioneffect variegation and Polycomb group (PC-G) genes have been useful tools to investi[r] ...
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Accumulation of Spliced Avian Retrovirus mRNA Is Inhibited in S-Adenosylmethionine-Depleted Chicken Embryo Fibroblasts
... To RNA levels of mRNA different levelseofmRN methylation with the effects on the processing of viral RNA, we treated td B77 ASV-infected cells with various concentrations of cycloleucine[r] ...
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Association of neural tube defects with maternal alterations and genetic polymorphisms in one-carbon metabolic pathway
... AdoHcy: S-adenosylhomocysteine; AdoMet: S-adenosylmethionine; BHMT: Betaine-homocysteine methyltransferase; CBS: Cystathionine beta synthase; Hcy: Homocysteine; MTHFD1: Methylenetetrahyd[r] ...
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Novel liquid chromatography based assays for s-adenosylmethionine and related metabolites
... brain spm synthase which is unaffected by SAM and SAH, the rat prostate spm and spd synthases were reported by the same reference to be inhibited by 100 pM SAM 30% and 75% respectively; whereas both enzymes were less ...
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Effect of S-adenosylmethionine on human rotavirus RNA synthesis.
... In vitro RNA synthesis by preincubated virions' [3H]UMP AmtAmt of of [3H]UMP Preincubation incorporated into condition acid-insoluble Additions after virus reisolation condition acid-ins[r] ...
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Immunoassay of S adenosylmethionine and S adenosylhomocysteine: the methylation index as a biomarker for disease and health status
... and S-adenosylmethio- nine (facilitates glutathione usage and maintains acetyl- choline levels) enhances or maintains cognitive function and attenuates or prevents aggression, in mouse models of aging and ...
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Fe-Hydrogenase Maturases in the Hydrogenosomes of Trichomonas vaginalis
... Synthesis and insertion of the metal clusters at the active centers of NiFe-hydrogenase apoproteins and proteolytic pro- cessing after completion of the active centers in bacteria re- quire the action of an independent ...
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