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SA-β-gal

MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

... in SA-β-gal positive cell percentages and G1 phase-arrested cell percentages, as well as an increase in p16, p53, and p21 protein levels in PUVA- and UVB-SIPS fibroblasts transfected by Ago-CNT ...

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Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

... Current evidence implicates intervertebral disc degeneration as a major cause of low back pain, although its pathogenesis is poorly understood. Numerous characteristic features of disc degeneration mimic those seen ...

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Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro

Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro

... Epidemiological studies have shown that cigarette smoke (CS), a very common environmental factor, plays an important role in skin aging. Although some in vivo studies have suggested that CS affects skin aging, the ...

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High magnitude compression accelerates the premature senescence of nucleus pulposus cells via the p38 MAPK ROS pathway

High magnitude compression accelerates the premature senescence of nucleus pulposus cells via the p38 MAPK ROS pathway

... associated β -galactosidase (SA- β -Gal) activity, glycosaminoglycan (GAG) content, matrix macromolecule (aggrecan and col- lagen II) expression and senescence marker (p16 and p53) ...

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MDM2 turnover and expression of ATRX determine the choice between quiescence and senescence in response to CDK4 inhibition

MDM2 turnover and expression of ATRX determine the choice between quiescence and senescence in response to CDK4 inhibition

... associated β-galactosidase (SA-β-gal, Figure 1C) and focal HP1γ, a marker of senescence associated heterochromatic foci (SAHF, Figure 1D), increased only in LS8817, LS141 and LS0082 ...

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Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

... During ageing and degeneration, the matrix of the IVD under- goes substantial structural, molecular, and mechanical changes, including a loss in the demarcation between the annulus fibrosus (AF) and the nucleus pulposus ...

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Is senescence-associated β-galactosidase a marker of neuronal senescence?

Is senescence-associated β-galactosidase a marker of neuronal senescence?

... associated- β-galactosidase ...noticeable SA-β-gal activity quite early in culture. Many SA-β-gal-positive neurons were negative for another canonical marker of ...

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Retroviral transfer of the p16INK4a cDNA inhibits C6 glioma formation in Wistar rats

Retroviral transfer of the p16INK4a cDNA inhibits C6 glioma formation in Wistar rats

... We have observed a morphological alteration of the C6 cells upon infection with the pCLp16 virus. The control cells surviving G418 selection in the colony formation as- says (as in Figure 1) were not altered ...

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The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D Galactose induced aged rat model

The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D Galactose induced aged rat model

... senescence-associated β -galactosidase (SA- β -gal) staining, flow cytometric cell cycle phase distribution assay, CCK-8 cell proliferation assay, oxidative stress (reactive oxygen species ...

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Immune response to RB1 regulated senescence limits radiation induced osteosarcoma formation

Immune response to RB1 regulated senescence limits radiation induced osteosarcoma formation

... senescence-associated β-galactosidase (SA-β-Gal; Figure 2C), increased numbers of flattened cells, and expression of CDKN2A, CDKN1A, and trimethylated H3K9 (Supplemental Figure ...by ...

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Proof of principle: quality control of therapeutic cell preparations using senescence associated DNA methylation changes

Proof of principle: quality control of therapeutic cell preparations using senescence associated DNA methylation changes

... Furthermore, SA-β-Gal may also be induced upon stress during in vitro culture [13] but this analysis does not facilitate reliable estimation of cumulative population doublings ...

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NQO1 Stabilizes p53 in Response to Oncogene-Induced Senescence

NQO1 Stabilizes p53 in Response to Oncogene-Induced Senescence

... (G) IMR90 cells stably expressing shRNAs against NQO1 upon exposure to Ras G12V for 5d were assessed for SA- β -gal activity and BrdU incorporation... formed BrdU incorporation assay t[r] ...

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Gender disparity in LDL-induced cardiovascular damage and the protective role of estrogens against electronegative LDL

Gender disparity in LDL-induced cardiovascular damage and the protective role of estrogens against electronegative LDL

... with SA-β-gal. SA-β-gal activity, indicated by blue color, was strong in aortas of male db/db mice, weak in aortas of female db/db mice, and absent in aortas of male or female ...

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The Oct1 homolog Nubbin is a repressor of NF-κB-dependent immune gene expression that increases the tolerance to gut microbiota

The Oct1 homolog Nubbin is a repressor of NF-κB-dependent immune gene expression that increases the tolerance to gut microbiota

... Surprisingly, we observed prominent β -galactosidase ( β -gal) reporter stain- ing in the fat body of uninfected nub 1 ; CecA1-lacZ flies (Figure 1B) compared to the control flies (Figur[r] ...

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How to utilize Ca2+signals to rejuvenate the repairative phenotype of senescent endothelial progenitor cells in elderly patients affected by cardiovascular diseases: a useful therapeutic support of surgical approach?

How to utilize Ca2+signals to rejuvenate the repairative phenotype of senescent endothelial progenitor cells in elderly patients affected by cardiovascular diseases: a useful therapeutic support of surgical approach?

... Furthermore, they express senescence-associated enzymes, such as the acidic b -galactosidase (SA- b -gal). Last, but not least, age- ing ECs loose their capability to proliferate and to [r] ...

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An Org 1–Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila

An Org 1–Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila

... for β 3-Tub, tupAME-moeGFP (see description below) and β PS integrin showed β PS accumulation both at the AM epidermal attachment site and at dorsal projections in contact with either the PSC or ...

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The GTPase domain of Galphao contributes to the functional interaction of Galphao with the promyelocytic leukemia zinc finger protein

The GTPase domain of Galphao contributes to the functional interaction of Galphao with the promyelocytic leukemia zinc finger protein

... Abbreviations used: β -gal – β -galactosidase; BrdU – bromodeoxyuridine; G α o – the alpha subunit of Go; G proteins – heterotrimeric GTP-binding proteins; GST – glutathione- S - tran[r] ...

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Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche

Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche

... expressed Ki67 (in other words they proliferated; Fig. 3a, b). As we have previously documented, Sdf-1 did not influ- ence proliferation of cells [48]. After 5 days of coculture, 56.7% of CXCR4 + BMCs(β-gal ...

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Intrahepatic cholangiocarcinoma can arise from Notch mediated conversion of hepatocytes

Intrahepatic cholangiocarcinoma can arise from Notch mediated conversion of hepatocytes

... to induce ICC (10, 11). After 30 weeks of TAA administration, all of the treated mice developed macronodular liver cirrhosis (Figure 1B). Immunohistochemical and histochemical analy- ses revealed that many primitive ...

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18-beta-glycyrrhetinic Acid Protects against Staphylococcus aureus Infection by Regulating the NF-κB Pathway

18-beta-glycyrrhetinic Acid Protects against Staphylococcus aureus Infection by Regulating the NF-κB Pathway

... Figure 3: 18β-GA reduces HMGB1 expression and anti-HMGB1 increases the mice survival rates. Mice were intraperitoneal in- jected with either 18β-GA (50 mg/kg) or PBS 2 h before infected with SA (1×108 CFU). Mice ...

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